1641
Nucleic Acids Research, Vol. 18, No. 6
Xenopus laevis Cu,Zn superoxide dismutase B cDNA sequence Maria Teresa Carri, Andrea Battistoni, Paolo Mariottini and Giuseppe Rotilio Dipartimento di Biologia, 11 Universita di Roma 'Tor Vergata', Via E. Carnevale, 00173 Roma, Italy EMBL accession no. X51518
Submitted February 5, 1990
Cu,Zn superoxide dismutase (SOD) is one of the cytoplasmic enzymes involved in cell detoxification from reactive oxygen species. It has been previously shown (1) that X. kaevis cells express two variants of this enzyme (XSODA and XSODB). We report here the complete nucleotide sequence of XSODB. The deduced amino acid sequence differs from the reported protein sequence (1), in which Pro was found in position 60 instead of Ser. A X. laevis embryos (stage 40) (2) cDNA library was screened using a cDNA coding for XSODA as a probe (3). Two positive clones proved to contain the whole XSODB coding sequence, together with a short 5' non-coding segment and the whole 3' segment to the polyA tail. The ATG start codon, the TGA stop and a possible AATAAA polyadenylation signal are indicated.
ACKNOWLEDGEMENTS We thank Dr G.Spohr for kindly making available the cDNA library and Mr A.Giurin for technical help. This work was partially supported by C.N.R; Project 'Biotecnologie e Biostrumentazione'.
REFERENCES 1. Schinina,M.E. et al. (1989) Arch. Biochem. Biophys. 272, 507-515. 2. Stutz,F. and Spohr,G. (1986) J. Mol. Biol. 187, 349-361. 3. Montesano,L. et al. (1989) Eur. J. Biochem. 186, 421-426.
1 aactctccagtgtctgtctgtcagtagttgtgctgcaaccATGGTGAAGGCAGTGTGTGT 60 *
V
K
A
V
C
V
61 GCTGGCGGGAAGCGGCGATGTGAAGGGAGTGGTGCACTTCGAGCAACAGGATGAAGGAGC 120 L A G S G D V K G V V H F EQ Q D E G A 121 AGTGAGTGTGGAAGGGAAGATCGAGGGACTGACTGACGGACTGCACGGCTTCCATATTCA 180 V S V E G K I E G L T D G L H G F H I H 181 TGTGTTTGGGGACAACACTAATGGCTGCATGAGCGCTGGATCTCATTTCAACCCCGAGAA 240 V F G D N T N G C M S A G S H F N P E N 241 CAAGAATCACGGAGCCCCGGGAGATACAGACAGGCACGTGGGAGACCTGGGCAATGTGAC 300 K N H G A P G D T D R H V G D L G N V T
301 GGCTGAAGGTGGAGTCGCTCAGTTCAAAATCACAGATTCTCTGATCTCCCTGAAAGGCCC 360 A E G G V A Q F K I T D S L I S L K G P 361 AAACTCCATCATTGGCCGCACTGCTGTGGTCCATGAGAAGGCAGACGACTTGGGCAAAGG 420 N S I I G R T A V V H E K A D D L G K G 421
TGGAAATGATGAGAGTCTGAAAACGGGCAATGCTGGGGGTCGTCTGGCTTGTGGTGTGAT N
G
481
E
S
L
K
T
G
N
A
G
G
R
L
A
C
G
480
V I
CGGTTATAGTCCGTGAtcgactcccccgaagcatttagtctccttgttgtctcatcggca 540 G
541
D
Y
S
P
*
cttgttctccttctctccgcactcagttaccgctctccattcttcttgtgtttatctgac
601 tgaataaaggacacacctttgtcaccaaaaaaaaaaaaaa *
*
*
600
Nucleic Acids Research, Vol. 18, No. 6
Xenopus laevis Cu,Zn superoxide dismutase B cDNA sequence Maria Teresa Carri, Andrea Battistoni, Paolo Mariottini and Giuseppe Rotilio Dipartimento di Biologia, 11 Universita di Roma 'Tor Vergata', Via E. Carnevale, 00173 Roma, Italy EMBL accession no. X51518
Submitted February 5, 1990
Cu,Zn superoxide dismutase (SOD) is one of the cytoplasmic enzymes involved in cell detoxification from reactive oxygen species. It has been previously shown (1) that X. kaevis cells express two variants of this enzyme (XSODA and XSODB). We report here the complete nucleotide sequence of XSODB. The deduced amino acid sequence differs from the reported protein sequence (1), in which Pro was found in position 60 instead of Ser. A X. laevis embryos (stage 40) (2) cDNA library was screened using a cDNA coding for XSODA as a probe (3). Two positive clones proved to contain the whole XSODB coding sequence, together with a short 5' non-coding segment and the whole 3' segment to the polyA tail. The ATG start codon, the TGA stop and a possible AATAAA polyadenylation signal are indicated.
ACKNOWLEDGEMENTS We thank Dr G.Spohr for kindly making available the cDNA library and Mr A.Giurin for technical help. This work was partially supported by C.N.R; Project 'Biotecnologie e Biostrumentazione'.
REFERENCES 1. Schinina,M.E. et al. (1989) Arch. Biochem. Biophys. 272, 507-515. 2. Stutz,F. and Spohr,G. (1986) J. Mol. Biol. 187, 349-361. 3. Montesano,L. et al. (1989) Eur. J. Biochem. 186, 421-426.
1 aactctccagtgtctgtctgtcagtagttgtgctgcaaccATGGTGAAGGCAGTGTGTGT 60 *
V
K
A
V
C
V
61 GCTGGCGGGAAGCGGCGATGTGAAGGGAGTGGTGCACTTCGAGCAACAGGATGAAGGAGC 120 L A G S G D V K G V V H F EQ Q D E G A 121 AGTGAGTGTGGAAGGGAAGATCGAGGGACTGACTGACGGACTGCACGGCTTCCATATTCA 180 V S V E G K I E G L T D G L H G F H I H 181 TGTGTTTGGGGACAACACTAATGGCTGCATGAGCGCTGGATCTCATTTCAACCCCGAGAA 240 V F G D N T N G C M S A G S H F N P E N 241 CAAGAATCACGGAGCCCCGGGAGATACAGACAGGCACGTGGGAGACCTGGGCAATGTGAC 300 K N H G A P G D T D R H V G D L G N V T
301 GGCTGAAGGTGGAGTCGCTCAGTTCAAAATCACAGATTCTCTGATCTCCCTGAAAGGCCC 360 A E G G V A Q F K I T D S L I S L K G P 361 AAACTCCATCATTGGCCGCACTGCTGTGGTCCATGAGAAGGCAGACGACTTGGGCAAAGG 420 N S I I G R T A V V H E K A D D L G K G 421
TGGAAATGATGAGAGTCTGAAAACGGGCAATGCTGGGGGTCGTCTGGCTTGTGGTGTGAT N
G
481
E
S
L
K
T
G
N
A
G
G
R
L
A
C
G
480
V I
CGGTTATAGTCCGTGAtcgactcccccgaagcatttagtctccttgttgtctcatcggca 540 G
541
D
Y
S
P
*
cttgttctccttctctccgcactcagttaccgctctccattcttcttgtgtttatctgac
601 tgaataaaggacacacctttgtcaccaaaaaaaaaaaaaa *
*
*
600
