Accepted Manuscript Wee1B depletion promotes nuclear maturation of canine oocytes Yu-Gon Kim, Dong-Hoon Kim, Seok-Hwan Song, Kyeong-Lim Lee, Byoung-Chul Yang, Jeong Su Oh, Sang-Ryeul Lee, Il-Keun Kong PII:
S0093-691X(14)00564-0
DOI:
10.1016/j.theriogenology.2014.10.017
Reference:
THE 12963
To appear in:
Theriogenology
Received Date: 2 June 2014 Revised Date:
13 October 2014
Accepted Date: 18 October 2014
Please cite this article as: Kim Y-G, Kim D-H, Song S-H, Lee K-L, Yang B-C, Oh JS, Lee S-R, Kong I-K, Wee1B depletion promotes nuclear maturation of canine oocytes, Theriogenology (2014), doi: 10.1016/ j.theriogenology.2014.10.017. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT 1
To: THERIOGENOLOGY
“Revised highlighted”
2
Wee1B depletion promotes nuclear maturation of canine oocytes
3
RI PT
4
Yu-Gon Kima,†, Dong-Hoon Kimc,†, Seok-Hwan Songa, Kyeong-Lim Leea, Byoung-Chul
6
Yangc, Jeong Su Ohd, Sang-Ryeul Leee, and Il-Keun Konga,b,†
SC
5
7 a
Department of Animal Science, Division of Applied Life Science, bInstitute of Agriculture and
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Life Science, Gyeongsang National University, Jinju 660-701, Gyeongsangnam-do, Republic
10
of Korea, cAnimal Biotechnology Division, National Institute of Animal Science, Suwon 441-
11
706, Gyeonggi-do, Republic of Korea, dDepartment of Genetic Engineering, College of
12
Biotechnology and Bioengineering, Sungkyunkwan University, Gyeonggi-do 440-746,
13
Republic of Korea, eAnimal, Dairy, and Veterinary Sciences Department, Utah State University,
14
Logan, UT 84322-4700, USA
15
16
17
†
18
†
AC C
EP
TE D
M AN U
8
These authors contributed equally to this work. Corresponding author: Tel: +82-(0)55-772-1942, Email: [email protected]
1
ACCEPTED MANUSCRIPT 19
ABSTRACT
20
Most mammalian oocytes are arrested at the germinal vesicle (GV) stage by
22
activation of Wee1B. Meiotic resumption is regulated by inactivation of Wee1B and
23
activation of cell division cycle 25B (cdc25B). The aim of this study was to determine
24
whether treatment with Wee1B-targeting small interfering RNA (Wee1B-siRNA) promotes
25
nuclear maturation of canine oocytes from GV stage to metaphase II (MII) stage. In
26
Experiment 1, the percentage of canine oocytes that matured to MII stage was higher (P
S0093-691X(14)00564-0
DOI:
10.1016/j.theriogenology.2014.10.017
Reference:
THE 12963
To appear in:
Theriogenology
Received Date: 2 June 2014 Revised Date:
13 October 2014
Accepted Date: 18 October 2014
Please cite this article as: Kim Y-G, Kim D-H, Song S-H, Lee K-L, Yang B-C, Oh JS, Lee S-R, Kong I-K, Wee1B depletion promotes nuclear maturation of canine oocytes, Theriogenology (2014), doi: 10.1016/ j.theriogenology.2014.10.017. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT 1
To: THERIOGENOLOGY
“Revised highlighted”
2
Wee1B depletion promotes nuclear maturation of canine oocytes
3
RI PT
4
Yu-Gon Kima,†, Dong-Hoon Kimc,†, Seok-Hwan Songa, Kyeong-Lim Leea, Byoung-Chul
6
Yangc, Jeong Su Ohd, Sang-Ryeul Leee, and Il-Keun Konga,b,†
SC
5
7 a
Department of Animal Science, Division of Applied Life Science, bInstitute of Agriculture and
9
Life Science, Gyeongsang National University, Jinju 660-701, Gyeongsangnam-do, Republic
10
of Korea, cAnimal Biotechnology Division, National Institute of Animal Science, Suwon 441-
11
706, Gyeonggi-do, Republic of Korea, dDepartment of Genetic Engineering, College of
12
Biotechnology and Bioengineering, Sungkyunkwan University, Gyeonggi-do 440-746,
13
Republic of Korea, eAnimal, Dairy, and Veterinary Sciences Department, Utah State University,
14
Logan, UT 84322-4700, USA
15
16
17
†
18
†
AC C
EP
TE D
M AN U
8
These authors contributed equally to this work. Corresponding author: Tel: +82-(0)55-772-1942, Email: [email protected]
1
ACCEPTED MANUSCRIPT 19
ABSTRACT
20
Most mammalian oocytes are arrested at the germinal vesicle (GV) stage by
22
activation of Wee1B. Meiotic resumption is regulated by inactivation of Wee1B and
23
activation of cell division cycle 25B (cdc25B). The aim of this study was to determine
24
whether treatment with Wee1B-targeting small interfering RNA (Wee1B-siRNA) promotes
25
nuclear maturation of canine oocytes from GV stage to metaphase II (MII) stage. In
26
Experiment 1, the percentage of canine oocytes that matured to MII stage was higher (P
