Accepted Manuscript Vitamin D deficiency is associated with disability and disease progression in multiple sclerosis patients independently of oxidative and nitrosative stress

Sayonara R. Oliveira, Andréa N.C. Simão, Daniela Frizon Alfieri, Tamires Flauzino, Ana Paula Kallaur, Leda Mezzaroba, Marcell Alysson B. Lozovoy, Beatriz Sardinha Sabino, Katerine Panichi Zanin Ferreira, Wildea L.C.J. Pereira, Damacio R. KaimenMaciel, Isaias Dichi, Edna M.V. Reiche PII: DOI: Reference:

S0022-510X(17)30480-X doi: 10.1016/j.jns.2017.07.046 JNS 15470

To appear in:

Journal of the Neurological Sciences

Received date: Revised date: Accepted date:

9 November 2016 10 July 2017 29 July 2017

Please cite this article as: Sayonara R. Oliveira, Andréa N.C. Simão, Daniela Frizon Alfieri, Tamires Flauzino, Ana Paula Kallaur, Leda Mezzaroba, Marcell Alysson B. Lozovoy, Beatriz Sardinha Sabino, Katerine Panichi Zanin Ferreira, Wildea L.C.J. Pereira, Damacio R. Kaimen-Maciel, Isaias Dichi, Edna M.V. Reiche , Vitamin D deficiency is associated with disability and disease progression in multiple sclerosis patients independently of oxidative and nitrosative stress. The address for the corresponding author was captured as affiliation for all authors. Please check if appropriate. Jns(2017), doi: 10.1016/j.jns.2017.07.046

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ACCEPTED MANUSCRIPT VITAMIN D DEFICIENCY IS ASSOCIATED WITH DISABILITY AND DISEASE PROGRESSION IN MULTIPLE SCLEROSIS PATIENTS INDEPENDENTLY OF OXIDATIVE AND NITROSATIVE STRESS Sayonara R Oliveira1 , Andréa NC Simão1 , Daniela Frizon Alfieri2 , Tamires Flauzino2 , Ana Paula Kallaur2 , Leda Mezzaroba1 , Marcell Alysson B Lozovoy1 , Beatriz Sardinha Sabino3 , Katerine Panichi Zanin Ferreira2 , Wildea LCJ Pereira2 , Damacio R Kaimen-

Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center,

2

SC

State University of Londrina, Londrina, Paraná, Brazil;

Postgraduate Program, Health Sciences Center, State University of Londrina,

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Londrina, Paraná, Brazil; 3

Pharmacy School, Health Sciences Center, State University of Londrina; Londrina,

Paraná, Brazil.

Department of Clinical Medicine, Health Sciences Center, State University of

Londrina, Londrina, Paraná, Brazil;

Outpatient Clinic of Demyelinating Diseases, University Hospital, State University of

ED

5

MA

4

RI

1

PT

Maciel4,5 , Isaias Dichi4 , Edna MV Reiche1

Londrina; Londrina, Paraná, Brazil.

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Correspondence author: Andréa Name Colado Simão. Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil; [email protected]. Phone: +55-43-3371-

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2321; FAX: +55-43-3371-2619

ACCEPTED MANUSCRIPT Abstract The aim of this study was to assess vitamin D status in patients with multiple sclerosis (MS) and to evaluate whether it was associated with oxidative and nitrosative stress (O&NS) markers and disability. This study included 137 patients with MS and 218 healthy controls. The markers evaluated were serum levels of 25-hydroxyvitamin D, lipid hydroperoxides, advanced oxidation protein products (AOPP), nitric oxide metabolites (NOx), and total radical-trapping antioxidant parameter TRAP/UA. Patients

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with 25(OH)D