Acta Microbiologica et Immunologica Hungarica, 61 (4), pp. 425–434 (2014) DOI: 10.1556/AMicr.61.2014.4.4
VIRULENCE FACTORS, ANTIMICROBIAL SUSCEPTIBILITY AND MOLECULAR CHARACTERIZATION OF STREPTOCOCCUS AGALACTIAE ISOLATED FROM PREGNANT WOMEN REZA BEIGVERDI1, FERESHTEH JABALAMELI1, AKBAR MIRSALEHIAN1, SEDIGHEH HANTOUSHZADEH2, SHAHRAM BOROUMANDI3, MOROVAT TAHERIKALANI4 and MOHAMMAD EMANEINI1* 1
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2 Maternal, Fetal and Neonatal Research Center, Vali-asr Hospital, Tehran University of Medical Sciences, Tehran, Iran 3 Department of Microbiology, Pars Hospital, Tehran, Iran 4 Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
(Received: July 21, 2014; accepted: October 9, 2014)
Forty-one Streptococcus agalactiae isolates collected from pregnant women at 35–37 weeks of gestation were analysed for their capsular types, antimicrobial resistance determinants, distribution of virulence factors and genetic relatedness using PCR and multiplex PCR. Capsular type III was predominant (65.8%), followed by capsular type II (14.6%), Ib (7.3%), and V (4.9%). All isolates were susceptible to penicillin, vancomycin, linezolid and quinupristin-dalfopristin. Resistance to tetracycline, erythromycin and clindamycin were found in 97.6%, 24.4%, and 14.6% of isolates, respectively. The most common antimicrobial resistance gene was tetM found in 97.6% of the isolates followed by ermTR and ermB found in 12% and 7.3% of isolates, respectively. The most common virulence gene was hly (100%), followed by scpB (97.6%), bca (97.6%), rib (53.65%) and bac (4.9%). The insertion sequence IS1548 was found in 63.4% of isolates. By multi locus variable number of tandem repeat analysis (MLVA) typing, 30 different allelic profiles or MLVA types (MTs) were identified. The most frequent was the MT1 (5/41, 12.2%) and followed by MT2 (4/41, 9.75%). Our data revealed that population structure of these isolates is highly diverse and indicates different MLVA types. Keywords: Streptococcus agalactiae, MLVA type, virulence factors, antimicrobial resistance
* Corresponding author; E-mails: [email protected], [email protected]
1217-8950/$20.00 © 2014 Akadémiai Kiadó, Budapest
VIRULENCE FACTORS, ANTIMICROBIAL SUSCEPTIBILITY AND MOLECULAR CHARACTERIZATION OF STREPTOCOCCUS AGALACTIAE ISOLATED FROM PREGNANT WOMEN REZA BEIGVERDI1, FERESHTEH JABALAMELI1, AKBAR MIRSALEHIAN1, SEDIGHEH HANTOUSHZADEH2, SHAHRAM BOROUMANDI3, MOROVAT TAHERIKALANI4 and MOHAMMAD EMANEINI1* 1
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2 Maternal, Fetal and Neonatal Research Center, Vali-asr Hospital, Tehran University of Medical Sciences, Tehran, Iran 3 Department of Microbiology, Pars Hospital, Tehran, Iran 4 Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
(Received: July 21, 2014; accepted: October 9, 2014)
Forty-one Streptococcus agalactiae isolates collected from pregnant women at 35–37 weeks of gestation were analysed for their capsular types, antimicrobial resistance determinants, distribution of virulence factors and genetic relatedness using PCR and multiplex PCR. Capsular type III was predominant (65.8%), followed by capsular type II (14.6%), Ib (7.3%), and V (4.9%). All isolates were susceptible to penicillin, vancomycin, linezolid and quinupristin-dalfopristin. Resistance to tetracycline, erythromycin and clindamycin were found in 97.6%, 24.4%, and 14.6% of isolates, respectively. The most common antimicrobial resistance gene was tetM found in 97.6% of the isolates followed by ermTR and ermB found in 12% and 7.3% of isolates, respectively. The most common virulence gene was hly (100%), followed by scpB (97.6%), bca (97.6%), rib (53.65%) and bac (4.9%). The insertion sequence IS1548 was found in 63.4% of isolates. By multi locus variable number of tandem repeat analysis (MLVA) typing, 30 different allelic profiles or MLVA types (MTs) were identified. The most frequent was the MT1 (5/41, 12.2%) and followed by MT2 (4/41, 9.75%). Our data revealed that population structure of these isolates is highly diverse and indicates different MLVA types. Keywords: Streptococcus agalactiae, MLVA type, virulence factors, antimicrobial resistance
* Corresponding author; E-mails: [email protected], [email protected]
1217-8950/$20.00 © 2014 Akadémiai Kiadó, Budapest
