Environ Sci Pollut Res DOI 10.1007/s11356-014-3552-x

RESEARCH ARTICLE

Variation of antibiotic resistance genes in municipal wastewater treatment plant with A2O-MBR system Jing Du & Jinju Geng & Hongqiang Ren & Lili Ding & Ke Xu & Yan Zhang

Received: 9 May 2014 / Accepted: 3 September 2014 # Springer-Verlag Berlin Heidelberg 2014

Abstract The variation of five antibiotic resistance genes (ARGs)—tetG, tetW, tetX, sul1, and intI1—in a full-scale municipal wastewater treatment plant with A2O-MBR system was studied. The concentrations of five resistance genes both in influent and in membrane bioreactor (MBR) effluent decreased as sul1>intI1>tetX>tetG>tetW, and an abundance of sul1 was statistically higher than three other tetracycline resistance genes (tetG, tetW, and tetX) (p tetG (influent 105.78 copies mL−1; effluent 103.58 copies mL−1)>tetW (influent 105.75 copies mL−1; effluent 102.71 copies mL−1). Independent t test showed that concentration of sul1 was significantly higher than the three tetracycline resistance genes (tetG, tetW, and tetX) both in influent and effluent (ptetW, and quantities of ARGs in wastewater medium decreased through treatment processes. The concentration of ARGs in influent reached to the peak value in spring. Three of them (tetW, tetX, and sul1) reached the lowest concentration in autumn. The concentration of ARGs in wastewater samples decreased successively along the anaerobic effluent and anoxic effluent but increased in the aerobic effluent. COD exhibited significant correlation with tetW and sul1 in influent, respectively. The concentration of ARGs (copies mg−1) in sludge samples increased along treatment process, but the abundance of these genes (normalized to 16S rDNA) changed little along the A2O process and increased in MBR process. MBR is more effective in the removal of ARGs from wastewater. Acknowledgments This work was supported by the National Science Foundation of China (No. 51278241) and the Jiangsu Natural Science Foundation (No. BK2011016).

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Variation of antibiotic resistance genes in municipal wastewater treatment plant with A(2)O-MBR system.

The variation of five antibiotic resistance genes (ARGs)-tetG, tetW, tetX, sul1, and intI1-in a full-scale municipal wastewater treatment plant with A...
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