RESEARCH ARTICLE
Vaginal versus Obstetric Infection Escherichia coli Isolates among Pregnant Women: Antimicrobial Resistance and Genetic Virulence Profile
a11111
Emma Sáez-López1,2, Elisabet Guiral1,2, Dietmar Fernández-Orth2, Sonia Villanueva2, Anna Goncé3,4,5,6, Marta López3,4,5,6, Irene Teixidó3,4,5,6, Anna Pericot3,4,5,6, Francesc Figueras3,4,5,6, Montse Palacio3,4,5,6, Teresa Cobo3,4,5,6, Jordi Bosch1,2, Sara M. Soto1,2* 1 Department of Microbiology, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain, 2 ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain, 3 BCNatal-Barcelona Center of Maternal-Fetal and Neonatal Medicine (Hospital Clínic and Hospital Sant Joan de Déu), Barcelona, Spain, 4 Institut D0 Investigacions Biomèdiques August Pi I Sunyer (DIBAPS), Barcelona, Spain, 5 University of Barcelona, Barcelona, Spain, 6 Center for Biomedical Research on Rare Diseases (CIBER-ER), Barcelona, Spain
OPEN ACCESS Citation: Sáez-López E, Guiral E, Fernández-Orth D, Villanueva S, Goncé A, López M, et al. (2016) Vaginal versus Obstetric Infection Escherichia coli Isolates among Pregnant Women: Antimicrobial Resistance and Genetic Virulence Profile. PLoS ONE 11(1): e0146531. doi:10.1371/journal.pone.0146531 Editor: Marcia Edilaine Lopes Consolaro, State University of Maringá/Universidade Estadual de Maringá, BRAZIL Received: October 27, 2015 Accepted: December 19, 2015 Published: January 19, 2016 Copyright: © 2016 Sáez-López et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper. Funding: Sara M. Soto has a fellowship from the program I3, of the Instituo de Salud Carlos III (ISCIII, www.isciii.es). This material is based upon work supported by the “Fondo de Investigaciones Sanitarias” (PI10/01579 and PI13/00137) integrated in the “Plan Nacional de I+D+I” and co-funded by the “ISCIII-Subdirección General de Evaluación” and the “Fondo Europeo de Desarrollo Regional (FEDER)”.
* [email protected]
Abstract Vaginal Escherichia coli colonization is related to obstetric infections and the consequent development of infections in newborns. Ampicillin resistance among E. coli strains is increasing, which is the main choice for treating empirically many obstetric and neonatal infections. Vaginal E. coli strains are very similar to extraintestinal pathogenic E. coli with regards to the virulence factors and the belonging to phylogroup B2. We studied the antimicrobial resistance and the genetic virulence profile of 82 E. coli isolates from 638 vaginal samples and 63 isolated from endometrial aspirate, placental and amniotic fluid samples from pregnant women with obstetric infections. The prevalence of E. coli in the vaginal samples was 13%, which was significant among women with associated risk factors during pregnancy, especially premature preterm rupture of membranes (p
Vaginal versus Obstetric Infection Escherichia coli Isolates among Pregnant Women: Antimicrobial Resistance and Genetic Virulence Profile
a11111
Emma Sáez-López1,2, Elisabet Guiral1,2, Dietmar Fernández-Orth2, Sonia Villanueva2, Anna Goncé3,4,5,6, Marta López3,4,5,6, Irene Teixidó3,4,5,6, Anna Pericot3,4,5,6, Francesc Figueras3,4,5,6, Montse Palacio3,4,5,6, Teresa Cobo3,4,5,6, Jordi Bosch1,2, Sara M. Soto1,2* 1 Department of Microbiology, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain, 2 ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain, 3 BCNatal-Barcelona Center of Maternal-Fetal and Neonatal Medicine (Hospital Clínic and Hospital Sant Joan de Déu), Barcelona, Spain, 4 Institut D0 Investigacions Biomèdiques August Pi I Sunyer (DIBAPS), Barcelona, Spain, 5 University of Barcelona, Barcelona, Spain, 6 Center for Biomedical Research on Rare Diseases (CIBER-ER), Barcelona, Spain
OPEN ACCESS Citation: Sáez-López E, Guiral E, Fernández-Orth D, Villanueva S, Goncé A, López M, et al. (2016) Vaginal versus Obstetric Infection Escherichia coli Isolates among Pregnant Women: Antimicrobial Resistance and Genetic Virulence Profile. PLoS ONE 11(1): e0146531. doi:10.1371/journal.pone.0146531 Editor: Marcia Edilaine Lopes Consolaro, State University of Maringá/Universidade Estadual de Maringá, BRAZIL Received: October 27, 2015 Accepted: December 19, 2015 Published: January 19, 2016 Copyright: © 2016 Sáez-López et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper. Funding: Sara M. Soto has a fellowship from the program I3, of the Instituo de Salud Carlos III (ISCIII, www.isciii.es). This material is based upon work supported by the “Fondo de Investigaciones Sanitarias” (PI10/01579 and PI13/00137) integrated in the “Plan Nacional de I+D+I” and co-funded by the “ISCIII-Subdirección General de Evaluación” and the “Fondo Europeo de Desarrollo Regional (FEDER)”.
* [email protected]
Abstract Vaginal Escherichia coli colonization is related to obstetric infections and the consequent development of infections in newborns. Ampicillin resistance among E. coli strains is increasing, which is the main choice for treating empirically many obstetric and neonatal infections. Vaginal E. coli strains are very similar to extraintestinal pathogenic E. coli with regards to the virulence factors and the belonging to phylogroup B2. We studied the antimicrobial resistance and the genetic virulence profile of 82 E. coli isolates from 638 vaginal samples and 63 isolated from endometrial aspirate, placental and amniotic fluid samples from pregnant women with obstetric infections. The prevalence of E. coli in the vaginal samples was 13%, which was significant among women with associated risk factors during pregnancy, especially premature preterm rupture of membranes (p
