Author's Accepted Manuscript
Stiffening Effects of riboflavin/UVA corneal collagen crosslinking is hydration dependent H. Hatami-Marbini, A. Rahimi
www.elsevier.com/locate/jbiomech
PII: DOI: Reference:
S0021-9290(15)00054-8 http://dx.doi.org/10.1016/j.jbiomech.2015.01.038 BM7003
To appear in:
Journal of Biomechanics
Accepted date: 25 January 2015 Cite this article as: H. Hatami-Marbini, A. Rahimi, Stiffening Effects of riboflavin/UVA corneal collagen crosslinking is hydration dependent, Journal of Biomechanics, http://dx.doi.org/10.1016/j.jbiomech.2015.01.038 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Stiffening Effects of Riboflavin/UVA Corneal Collagen Crosslinking is Hydration Dependent
H. Hatami-Marbini, A. Rahimi School of Mechanical and Aerospace Engineering, Oklahoma State University, Stillwater, OK 74078
Keywords: Corneal biomechanics, corneal collagen cross-linking, uniaxial tensile experiments, bovine cornea
Word count: 3270
Corresponding Author: Hamed Hatami-Marbini, Ph.D. 218 Engineering North School of Mechanical and Aerospace Engineering Oklahoma State University Stillwater, OK 74078-5016 Email: [email protected], Phone: 405-744-5900, Fax: 405-744-7873.
1
Abstract The collagen cross-linking is a relatively new treatment option for strengthening the cornea, delaying, and in some cases stopping the progression of keratoconus. The uniaxial tensile experiments are among the most commonly used techniques to assess the effectiveness of this therapeutic option in enhancing tensile properties. In the present study, we investigated the possible effects of hydration on stiffening effects of corneal collagen cross-linking procedure, as measured by the uniaxial tensile testing method. For this purpose, after cross-linking bovine corneas, we let the strips to dehydrate in air or swell in a solution until their thickness reached an average thickness of 0.5, 0.7, 0.9, 1.1, and 1.5 mm. Using thickness as a representative of hydration, we divided corneal strips into five different groups and measured their stress-strain behavior by conducting uniaxial tensile experiments in mineral oil. It was observed that the collagen cross-linking treatment and hydration together affect the tensile behavior of the bovine cornea. While corneal collagen cross-linking resulted in a significant increase in the tensile stress-strain response of each thickness group (P
Stiffening Effects of riboflavin/UVA corneal collagen crosslinking is hydration dependent H. Hatami-Marbini, A. Rahimi
www.elsevier.com/locate/jbiomech
PII: DOI: Reference:
S0021-9290(15)00054-8 http://dx.doi.org/10.1016/j.jbiomech.2015.01.038 BM7003
To appear in:
Journal of Biomechanics
Accepted date: 25 January 2015 Cite this article as: H. Hatami-Marbini, A. Rahimi, Stiffening Effects of riboflavin/UVA corneal collagen crosslinking is hydration dependent, Journal of Biomechanics, http://dx.doi.org/10.1016/j.jbiomech.2015.01.038 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Stiffening Effects of Riboflavin/UVA Corneal Collagen Crosslinking is Hydration Dependent
H. Hatami-Marbini, A. Rahimi School of Mechanical and Aerospace Engineering, Oklahoma State University, Stillwater, OK 74078
Keywords: Corneal biomechanics, corneal collagen cross-linking, uniaxial tensile experiments, bovine cornea
Word count: 3270
Corresponding Author: Hamed Hatami-Marbini, Ph.D. 218 Engineering North School of Mechanical and Aerospace Engineering Oklahoma State University Stillwater, OK 74078-5016 Email: [email protected], Phone: 405-744-5900, Fax: 405-744-7873.
1
Abstract The collagen cross-linking is a relatively new treatment option for strengthening the cornea, delaying, and in some cases stopping the progression of keratoconus. The uniaxial tensile experiments are among the most commonly used techniques to assess the effectiveness of this therapeutic option in enhancing tensile properties. In the present study, we investigated the possible effects of hydration on stiffening effects of corneal collagen cross-linking procedure, as measured by the uniaxial tensile testing method. For this purpose, after cross-linking bovine corneas, we let the strips to dehydrate in air or swell in a solution until their thickness reached an average thickness of 0.5, 0.7, 0.9, 1.1, and 1.5 mm. Using thickness as a representative of hydration, we divided corneal strips into five different groups and measured their stress-strain behavior by conducting uniaxial tensile experiments in mineral oil. It was observed that the collagen cross-linking treatment and hydration together affect the tensile behavior of the bovine cornea. While corneal collagen cross-linking resulted in a significant increase in the tensile stress-strain response of each thickness group (P
