crossmark THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 290, NO. 52, pp. 30697–30712, December 25, 2015 © 2015 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A.

Untargeted Plasma Metabolomics Identifies Endogenous Metabolite with Drug-like Properties in Chronic Animal Model of Multiple Sclerosis*□ S

Received for publication, July 23, 2015, and in revised form, October 29, 2015 Published, JBC Papers in Press, November 6, 2015, DOI 10.1074/jbc.M115.679068

Laila M. Poisson,a,b1 Hamid Suhail,c1 Jaspreet Singh,c Indrani Datta,a,b Aleksandar Denic,d Krzysztof Labuzek,e Md Nasrul Hoda,f,g Ashray Shankar,c Ashok Kumar,h Mirela Cerghet,c Stanton Elias,c Robert P. Mohney,i Moses Rodriguez,d,j2 Ramandeep Rattan,k Ashutosh K. Mangalam,l and Shailendra Giric3 From the aCenter for Bioinformatics and Departments of bPublic Health Sciences and cNeurology, and kDivision of Gynecology Oncology, Department of Women’s Health Services, Henry Ford Health System, Detroit, Michigan 48202, the Departments of d Neurology and jImmunology, Mayo Clinic College of Medicine, Rochester, Minnesota 55906, the eDepartment of Pharmacology, Medical University of Silesia, Medyków 18, PL 40-752 Katowice, Poland, the fDepartment of Neurology, Georgia Health Sciences University, Augusta, Georgia 30912, the gProgram in Clinical and Experimental Therapeutics, College of Pharmacy, University of Georgia, Augusta, Georgia 30912, the hDepartment of Anatomy and Cell Biology, School of Medicine, Wayne State University, Detroit, Michigan 48202, iMetabolon, Inc., Durham, North Carolina 27713, and the lDepartment of Pathology, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242 We performed untargeted metabolomics in plasma of B6 mice with experimental autoimmune encephalitis (EAE) at the chronic phase of the disease in search of an altered metabolic pathway(s). Of 324 metabolites measured, 100 metabolites that mapped to various pathways (mainly lipids) linked to mitochondrial function, inflammation, and membrane stability were observed to be significantly altered between EAE and control (p < 0.05, false discovery rate

Untargeted Plasma Metabolomics Identifies Endogenous Metabolite with Drug-like Properties in Chronic Animal Model of Multiple Sclerosis.

We performed untargeted metabolomics in plasma of B6 mice with experimental autoimmune encephalitis (EAE) at the chronic phase of the disease in searc...
NAN Sizes 2 Downloads 24 Views