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J Vasc Res. Author manuscript; available in PMC 2017 October 28. Published in final edited form as: J Vasc Res. 2016 ; 53(3-4): 196–205. doi:10.1159/000449201.
Transplantation of autologous bone marrow mononuclear cells regulates inflammation in a rabbit model of carotid artery atherosclerosis Kefei Cuia,*, Min Wanga, Lie Yub, Xiao RenC, Hui Cuia, Xiao fang Yua, Suyun Houa, Chao Fua, and Jian Wangd
Author Manuscript
aDepartment
of Ultrasound, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
bDepartment
of Neurology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052 Henan, China cDepartment
of Biochemistry and Molecular Biology, The First Clinical Medical College of Nanchang University, Nanchang, 330006, Jiangxi, China dDepartment
of Anesthesiology/Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA
Abstract Author Manuscript
Objective—It is well known that inflammation plays key roles in the development of atherosclerosis and that bone marrow mononuclear cells (BMMNCs) transplantation can suppress inflammation in rodent models of ischemic diseases. Here, we explored whether transplantation of autologous BMMNCs could prevent the progression of atherosclerosis by alleviation of inflammatory responses in a rabbit model of carotid artery atherosclerosis.
Author Manuscript
Methods and Results—The atherosclerotic rabbit model was established by air desiccation followed by a high-cholesterol diet for 8 weeks. Then 1×107 BMMNCs labeled with BrdU or equal volume of vehicle was injected into the rabbits via ear vein. Using ultrasonographic imaging method, we found that autologous BMMNCs treatment significantly decreased the area of atherosclerotic plaques compared to the vehicle-treated group (p0.05). 3.3. BMMNCs reduced area of carotid atherosclerotic plaque Using ultrasonographic imaging method, we found that the carotid arteries walls in naive and sham groups were smooth, no clear ultrasonographic plaque was found in these rabbits
J Vasc Res. Author manuscript; available in PMC 2017 October 28.
Cui et al.
Page 6
Author Manuscript
after 10 weeks of a high-cholesterol diet (fig.2A and B). In contrast, the carotid artery walls of rabbits in vehicle and BMMNC groups were rough, visible hyperechoic plaques were presented (fig.2C and D). In addition, the average diameter of plaque in vehicle treated group was significantly more than that in BMMNC treatment group (fig. 2C-1). Representative vessels taken from comparable regions of the carotid from naive-, sham-, vehicle- and BMMNC-treated rabbits were stained with HE (fig.2E–H). We found that autologous BMMNCs transplantation significantly reduced areas of atherosclerotic plaque compared to these in vehicle-treatment group (n=6/group, p
J Vasc Res. Author manuscript; available in PMC 2017 October 28. Published in final edited form as: J Vasc Res. 2016 ; 53(3-4): 196–205. doi:10.1159/000449201.
Transplantation of autologous bone marrow mononuclear cells regulates inflammation in a rabbit model of carotid artery atherosclerosis Kefei Cuia,*, Min Wanga, Lie Yub, Xiao RenC, Hui Cuia, Xiao fang Yua, Suyun Houa, Chao Fua, and Jian Wangd
Author Manuscript
aDepartment
of Ultrasound, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
bDepartment
of Neurology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052 Henan, China cDepartment
of Biochemistry and Molecular Biology, The First Clinical Medical College of Nanchang University, Nanchang, 330006, Jiangxi, China dDepartment
of Anesthesiology/Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA
Abstract Author Manuscript
Objective—It is well known that inflammation plays key roles in the development of atherosclerosis and that bone marrow mononuclear cells (BMMNCs) transplantation can suppress inflammation in rodent models of ischemic diseases. Here, we explored whether transplantation of autologous BMMNCs could prevent the progression of atherosclerosis by alleviation of inflammatory responses in a rabbit model of carotid artery atherosclerosis.
Author Manuscript
Methods and Results—The atherosclerotic rabbit model was established by air desiccation followed by a high-cholesterol diet for 8 weeks. Then 1×107 BMMNCs labeled with BrdU or equal volume of vehicle was injected into the rabbits via ear vein. Using ultrasonographic imaging method, we found that autologous BMMNCs treatment significantly decreased the area of atherosclerotic plaques compared to the vehicle-treated group (p0.05). 3.3. BMMNCs reduced area of carotid atherosclerotic plaque Using ultrasonographic imaging method, we found that the carotid arteries walls in naive and sham groups were smooth, no clear ultrasonographic plaque was found in these rabbits
J Vasc Res. Author manuscript; available in PMC 2017 October 28.
Cui et al.
Page 6
Author Manuscript
after 10 weeks of a high-cholesterol diet (fig.2A and B). In contrast, the carotid artery walls of rabbits in vehicle and BMMNC groups were rough, visible hyperechoic plaques were presented (fig.2C and D). In addition, the average diameter of plaque in vehicle treated group was significantly more than that in BMMNC treatment group (fig. 2C-1). Representative vessels taken from comparable regions of the carotid from naive-, sham-, vehicle- and BMMNC-treated rabbits were stained with HE (fig.2E–H). We found that autologous BMMNCs transplantation significantly reduced areas of atherosclerotic plaque compared to these in vehicle-treatment group (n=6/group, p
