AJEBAK 56 (Pt. 6) 737-739 (1978)

Brief Communication:

TOTAL PROTEIN PATTERNS IN HUMAN AMNIOTIC FLUID

by ARATI ROYCHOUDHURY. ARCHANA SHARMA' AND C E E T A TALUKDERf (From the Gytogenetics Laboratory, Department of Botany, University of Calcutta. Caleiitta-19 and fDepartmenl of Pathology, Institute of Postgraduate Medical Education and Research, Calcutta-20.)

(Accepted for publication Atifiitst 10, 1978.)

Summary. Total protein patterns of amniotic fluid samples of different age groups, following piilyacrylamide disc gd electrophoresis. showed a wide range of variation in early pregnancy, but no two cases had identical banding, indicating their genetic variability. An assessment has been made here of the total protein, albumin and transferrin patterns fn)m normal amniotic fluids of different age groups b\- gel electrophoresis, in order to observe the genetic variations and relationship with foetal age, if any.

MATERIALS AND METHODS

Amniotic fluids from foetuses between age groups of 4 to 27 and 38 to 40 weeks were subjected to vertical polyacr>'ianiide disc gel electrophoresis following a modification of the Davis (1964) and Omstein (1964) methods for 2!i li in a tris-glycine buffer, at a pH of 8 3 with a gel concentraticm KW. For transferrin, the method of Mueller. Smithies and Irwin (1962) was used. Serum from a normal adult was similarly processed as control.

RESULTS Total protein patterns of the 66 samples plotted cm a 10 cm scale (Smith. 1968), according to the age of the foetus, showed 3 to 11 bands in early and 14 to 17 hands in late pregnancy (Fig. 1). The bands were distributed within five reaignizable zones: A with /i-lipoproteiii; B with haptoglobin and transferrin; C with albumin and po.st-albinnin: D with pre-albumin; and E with Gc-globulin. Within each zone the patterns varied between individuals in number, intensity, mobility and width of bands.

Address for reprints.

738

A. ROYCHOUDHURY, A. SHARMA AND G. T A L U K D E R 2 PAIRS OF

TWINS 1 HAPTO GLOBIN I-TRANS FERRIN

c D

lO

I-ALBU MIN

NORMAL * ADULT SERUM Fig. 1.

4 - 15 WEEKS

Some samples of total protein pattern in human amniotic ffuids.

DISCUSSION Certain components of the amniotic fluid, like steroids, amino acids, proteins and some enzymes, have been studied by earlier workers (Abbas and Tovey, 1960; Emery et at, 1970; Fischbachcr and Quinlivan. 1970; Heron, 1966; Kang and Scanlon, 1974; Levy and Montag, 1969; Nadlfr and Burton. 1973; Roy Glioudhury. Sharma and Tatukder, 1975. 1978; Usategui-Gomez, Morgan and Toolan. 1966; Yliistalo et til., 1975). Amniotic fluids from normal women at term had earlier shown up to 21 bands which could not be related to the foetal age ( Fischbacher and Quinlivan, 1970). The number of l>ands obtained with paper and cellulose acetate electrophoresis was much less, ranging from 4 to 5. Amniotic Huid samples in the gestational periods between 15 weeks and term showed band.s in the Gc-giobulin region to be electrophoretically faster in all .samples (Johnson et ah. 1974). Observations made here indicate the presence of variable numbers of bands in the total protein pattern.s of .samples of different age groups. Variability was maximum in the zones B and C wliicli include haptoglobin. transferrin, post-albumin and albiuiiin. There was no change in transferrin phenot\pes. being CC in 55 samplfs, CB in 4 and CD in 1. which were entirely under genetic control. Albumin patterns exhiliited only a gradual increase in the overall mobility with increasing foetal age. There was no decrease in the amount of total protein as indicated by decrease in intensity and/or numbers of bands, The pattern was, however, much simpler than that of a normal adult serum with lower numbers of bands. The total protein patterns of amniotic (luid from normal pregnancies of different durations show a wide range of variations in number, widtli, intensity and mobilitj' of bands which are apparently due both to their inherent genetic make-up and stage of development. In late pregnancy. 14 to 17 bands are regularly seen. However, no two individual samples at any stage have identical patterns.

TOTAL PROTEIN PATTERNS IN HUMAN AMNIOTIC FLUID 739 Acknotcledgcments. The authors express their grateful thanks to Profe.s.sor A. K. Sharma and Professor K. P. Sen Gupta for encouragement and facilities provided, and to the Council of Scientific and Industrial Research, New Delhi, for the award of a Junior Fellowship to the first author. REFERENCES ABBAS, T , M . . and TOVEY, J. E. (1960):

NADLEH. H . L . , and BUBTON. B . K. (1973):

Br. Med. J.. 1. 476. DAVIS, B . J. (1964): Ann. N.Y. Acad. Sci., 121, 404.

In "Amniotic fluid: Research and Clinical Application", Excerpta Medica. Amsterdam, 1973. 1st edition, p. 223. OHNSTEIN, L. (1964): AMU, N.Y. Acad. Sci., 121. 321.

EMERY. A. E. H., BUBT. D . , ScniMCEouR.

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cet, 1. 1307. FiscKBACHKH. P. H.. and QUINLIVAN, W . L .

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and

TALUKDER. G . (1975): Pwc. Second All India Con^r. Genetics, 1975. /. Cijtol. Genet. Congr. Suppl., p. 151, ROY

CHOUDHUHY. A., TALUKDEH, G.. and

SHAHMA. A. (1978): Obstet. Gynecol. Siirt.. 33, 10. SMITH, 1. (1968): In "Ghromatographic and Electrophoretic Techniques", vol. 2. William Heinemann Medical Books Ltd., London; 1968. 1st edition. UsATEGUi-GoMEz, M.. MoRCAN. D. F., and TOOLAN. II. W. (1966): Proc. Soe. Exp. Biol. Med.. 123. 547. YLOSTALO, P., TUDERMAN, L.. KNUTTI. E .

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Total protein patterns in human amniotic fluid.

AJEBAK 56 (Pt. 6) 737-739 (1978) Brief Communication: TOTAL PROTEIN PATTERNS IN HUMAN AMNIOTIC FLUID by ARATI ROYCHOUDHURY. ARCHANA SHARMA' AND C E...
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