Funct Integr Genomics (2014) 14:351–362 DOI 10.1007/s10142-014-0374-3
ORIGINAL PAPER
The transcriptional network of WRKY53 in cereals links oxidative responses to biotic and abiotic stress inputs Leon Van Eck & Rebecca M. Davidson & Shuchi Wu & Bingyu Y. Zhao & Anna-Maria Botha & Jan E. Leach & Nora L. V. Lapitan
Received: 21 January 2014 / Revised: 14 April 2014 / Accepted: 16 April 2014 / Published online: 29 April 2014 # The Author(s) 2014. This article is published with open access at Springerlink.com
Abstract The transcription factor WRKY53 is expressed during biotic and abiotic stress responses in cereals, but little is currently known about its regulation, structure and downstream targets. We sequenced the wheat ortholog TaWRKY53 and its promoter region, which revealed extensive similarity in gene architecture and cis-acting regulatory elements to the rice ortholog OsWRKY53, including the presence of stressresponsive abscisic acid-responsive elements (ABRE) motifs and GCC-boxes. Four proteins interacted with the WRKY53 promoter in yeast one-hybrid assays, suggesting that this gene can receive inputs from diverse stress-related pathways such as calcium signalling and senescence, and environmental cues such as drought and ultraviolet radiation. The Ser/Thr receptor kinase ORK10/LRK10 and the apoplastic peroxidase POC1 are two downstream targets for regulation by the WRKY53
Electronic supplementary material The online version of this article (doi:10.1007/s10142-014-0374-3) contains supplementary material, which is available to authorized users. L. Van Eck : N. L. V. Lapitan (*) Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523, USA e-mail: [email protected] L. Van Eck : A.
ORIGINAL PAPER
The transcriptional network of WRKY53 in cereals links oxidative responses to biotic and abiotic stress inputs Leon Van Eck & Rebecca M. Davidson & Shuchi Wu & Bingyu Y. Zhao & Anna-Maria Botha & Jan E. Leach & Nora L. V. Lapitan
Received: 21 January 2014 / Revised: 14 April 2014 / Accepted: 16 April 2014 / Published online: 29 April 2014 # The Author(s) 2014. This article is published with open access at Springerlink.com
Abstract The transcription factor WRKY53 is expressed during biotic and abiotic stress responses in cereals, but little is currently known about its regulation, structure and downstream targets. We sequenced the wheat ortholog TaWRKY53 and its promoter region, which revealed extensive similarity in gene architecture and cis-acting regulatory elements to the rice ortholog OsWRKY53, including the presence of stressresponsive abscisic acid-responsive elements (ABRE) motifs and GCC-boxes. Four proteins interacted with the WRKY53 promoter in yeast one-hybrid assays, suggesting that this gene can receive inputs from diverse stress-related pathways such as calcium signalling and senescence, and environmental cues such as drought and ultraviolet radiation. The Ser/Thr receptor kinase ORK10/LRK10 and the apoplastic peroxidase POC1 are two downstream targets for regulation by the WRKY53
Electronic supplementary material The online version of this article (doi:10.1007/s10142-014-0374-3) contains supplementary material, which is available to authorized users. L. Van Eck : N. L. V. Lapitan (*) Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523, USA e-mail: [email protected] L. Van Eck : A.
