AGE (2014) 36:9699 DOI 10.1007/s11357-014-9699-z
The skeletal muscle satellite cell response to a single bout of resistance-type exercise is delayed with aging in men Tim Snijders & Lex B. Verdijk & Joey S. J. Smeets & Bryon R. McKay & Joan M. G. Senden & Fred Hartgens & Gianni Parise & Paul Greenhaff & Luc J. C. van Loon
Received: 21 February 2014 / Accepted: 30 July 2014 # American Aging Association 2014
Abstract Skeletal muscle satellite cells (SCs) have been shown to be instrumental in the muscle adaptive response to exercise. The present study determines agerelated differences in SC content and activation status following a single bout of exercise. Ten young (22± 1 years) and 10 elderly (73±1 years) men performed a single bout of resistance-type exercise. Muscle biopsies were collected before and 12, 24, 48, and 72 h after exercise. SC content and activation status were assessed in type I and type II muscle fibers by immunohistochemistry. Myostatin and MyoD protein and messenger RNA (mRNA) expression were determined by Western
blotting and rtPCR, respectively. In response to exercise, it took 48 h (young) and 72 h (elderly) for type II muscle fiber SC content to exceed baseline values (P
The skeletal muscle satellite cell response to a single bout of resistance-type exercise is delayed with aging in men Tim Snijders & Lex B. Verdijk & Joey S. J. Smeets & Bryon R. McKay & Joan M. G. Senden & Fred Hartgens & Gianni Parise & Paul Greenhaff & Luc J. C. van Loon
Received: 21 February 2014 / Accepted: 30 July 2014 # American Aging Association 2014
Abstract Skeletal muscle satellite cells (SCs) have been shown to be instrumental in the muscle adaptive response to exercise. The present study determines agerelated differences in SC content and activation status following a single bout of exercise. Ten young (22± 1 years) and 10 elderly (73±1 years) men performed a single bout of resistance-type exercise. Muscle biopsies were collected before and 12, 24, 48, and 72 h after exercise. SC content and activation status were assessed in type I and type II muscle fibers by immunohistochemistry. Myostatin and MyoD protein and messenger RNA (mRNA) expression were determined by Western
blotting and rtPCR, respectively. In response to exercise, it took 48 h (young) and 72 h (elderly) for type II muscle fiber SC content to exceed baseline values (P
