Journal of Chromatographic Science Advance Access published June 3, 2015 Journal of Chromatographic Science 2015;1– 7 doi:10.1093/chromsci/bmv064
Article
The Simple Determination Method for Anthocyanidin Aglycones in Fruits Using Ultra-High-Performance Liquid Chromatography You-Shin Shim1,2, Won-Jin Yoon1, Dong-Man Kim1, Masaki Watanabe3, Hyun-Jin Park4, Hae Won Jang1, Jangho Lee1,2 and Jaeho Ha1,2* 1
Korea Food Research Institute, 516, Baekhyeon, Bundang, Seongnam, Gyeonggi 463-746, Republic of Korea, 2Department of Food Biotechnology, University of Science and Technology, 217, Gajeong-ro, Yuseong-gu, Daejeon 305-350, Republic of Korea, 3Naka Division, Hitachi High-Tech Science Corporation, 11-1, Ishikawa-cho, Hitachinaka-shi, Ibaraki-ken 312-0057, Japan, and 4College of Life Sciences and Biotechnology, Korea University, Anam-Dong, Seongbuk-gu, Seoul 136-701, Republic of Korea *Author to whom correspondence should be addressed. Email: [email protected] Received 19 March 2014; revised 23 March 2015
The simple determination method for anthocyanidin aglycones in fruits using ultra-high-performance liquid chromatography (UHPLC) coupled with the heating-block acidic hydrolysis method was validated through the precision, accuracy and linearity. The UHPLC separation was performed on a reversed-phase C18 column ( particle size 2 mm, i.d. 2 mm, length 100 mm) with a photodiode-array detector. The limits of detection and quantification of the UHPLC analyses were 0.09 and 0.29 mg/kg for delphinidin, 0.08 and 0.24 mg/kg for cyanidin, 0.09 and 0.26 mg/kg for petunidin, 0.14 and 0.42 mg/kg for pelargonidin, 0.16 and 0.48 mg/kg for peonidin and 0.30 and 0.91 mg/kg for malvidin, respectively. The intra- and inter-day precisions of individual anthocyanidin aglycones were
Article
The Simple Determination Method for Anthocyanidin Aglycones in Fruits Using Ultra-High-Performance Liquid Chromatography You-Shin Shim1,2, Won-Jin Yoon1, Dong-Man Kim1, Masaki Watanabe3, Hyun-Jin Park4, Hae Won Jang1, Jangho Lee1,2 and Jaeho Ha1,2* 1
Korea Food Research Institute, 516, Baekhyeon, Bundang, Seongnam, Gyeonggi 463-746, Republic of Korea, 2Department of Food Biotechnology, University of Science and Technology, 217, Gajeong-ro, Yuseong-gu, Daejeon 305-350, Republic of Korea, 3Naka Division, Hitachi High-Tech Science Corporation, 11-1, Ishikawa-cho, Hitachinaka-shi, Ibaraki-ken 312-0057, Japan, and 4College of Life Sciences and Biotechnology, Korea University, Anam-Dong, Seongbuk-gu, Seoul 136-701, Republic of Korea *Author to whom correspondence should be addressed. Email: [email protected] Received 19 March 2014; revised 23 March 2015
The simple determination method for anthocyanidin aglycones in fruits using ultra-high-performance liquid chromatography (UHPLC) coupled with the heating-block acidic hydrolysis method was validated through the precision, accuracy and linearity. The UHPLC separation was performed on a reversed-phase C18 column ( particle size 2 mm, i.d. 2 mm, length 100 mm) with a photodiode-array detector. The limits of detection and quantification of the UHPLC analyses were 0.09 and 0.29 mg/kg for delphinidin, 0.08 and 0.24 mg/kg for cyanidin, 0.09 and 0.26 mg/kg for petunidin, 0.14 and 0.42 mg/kg for pelargonidin, 0.16 and 0.48 mg/kg for peonidin and 0.30 and 0.91 mg/kg for malvidin, respectively. The intra- and inter-day precisions of individual anthocyanidin aglycones were
