RESEARCH ARTICLE
The significance of elevated plasma expression of microRNA 106b~25 clusters in gastric cancer Fangxuan Li1,2,3,4☯, Yuenan Guo2,3,4,5☯, Juntian Liu1,2,3,4*, Rupeng Zhang2,3,4,5* 1 Department of Cancer Prevention Center, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China, 2 National Clinical Research Center for Cancer, Tianjin, China, 3 Key Laboratory of Cancer Prevention and Therapy, Tianjin, China, 4 Tianjin’s Clinical Research Center for Cancer, Tianjin, China, 5 Department of Gastric Cancer Surgery, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China
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☯ These authors contributed equally to this work. * [email protected] (RZ); [email protected] (JL)
Abstract Objective
OPEN ACCESS Citation: Li F, Guo Y, Liu J, Zhang R (2017) The significance of elevated plasma expression of microRNA 106b~25 clusters in gastric cancer. PLoS ONE 12(5): e0178427. https://doi.org/ 10.1371/journal.pone.0178427 Editor: Aamir Ahmad, University of South Alabama Mitchell Cancer Institute, UNITED STATES Received: December 20, 2016 Accepted: May 12, 2017 Published: May 31, 2017 Copyright: © 2017 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Funding: The work was supported by National Natural Science Fund of China (81502309). There was no additional external funding received for this study. Competing interests: The authors have declared that no competing interests exist.
Concentrating on oncogenic role and increased plasma expression of microRNA(miR) 106b~25 clusters (involving miR 106b, miR 93 and miR 25), we evaluated significance of the over-expression of plasma miR 106b~25 in GC.
Methods Based on 65 pairs matched GC patients and health controls, we explored clinical significance of miR 106b~25 for GC and compared their diagnostic performance with conventional tumor biomarkers including CA724, CA242, CA199 and CEA.
Results Both miR 106b~25 cluster and conventional tumor biomarkers were significantly elevated in GC (All P1 in 55/65 GC patients, shown in S2 Table. The expression of miR 106b, miR 93 and miR 25 were significant higher in poor differentiated GC and well differentiated GC than control groups. What’s more, miR 106b, miR 93 and miR 25 expression were significant higher in poor differentiated GC patient than well differentiated GC patients. Meanwhile, the concentration of CA724, CA242, CA199 and CEA were significant higher than healthy controls either (all P
The significance of elevated plasma expression of microRNA 106b~25 clusters in gastric cancer Fangxuan Li1,2,3,4☯, Yuenan Guo2,3,4,5☯, Juntian Liu1,2,3,4*, Rupeng Zhang2,3,4,5* 1 Department of Cancer Prevention Center, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China, 2 National Clinical Research Center for Cancer, Tianjin, China, 3 Key Laboratory of Cancer Prevention and Therapy, Tianjin, China, 4 Tianjin’s Clinical Research Center for Cancer, Tianjin, China, 5 Department of Gastric Cancer Surgery, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China
a1111111111 a1111111111 a1111111111 a1111111111 a1111111111
☯ These authors contributed equally to this work. * [email protected] (RZ); [email protected] (JL)
Abstract Objective
OPEN ACCESS Citation: Li F, Guo Y, Liu J, Zhang R (2017) The significance of elevated plasma expression of microRNA 106b~25 clusters in gastric cancer. PLoS ONE 12(5): e0178427. https://doi.org/ 10.1371/journal.pone.0178427 Editor: Aamir Ahmad, University of South Alabama Mitchell Cancer Institute, UNITED STATES Received: December 20, 2016 Accepted: May 12, 2017 Published: May 31, 2017 Copyright: © 2017 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Funding: The work was supported by National Natural Science Fund of China (81502309). There was no additional external funding received for this study. Competing interests: The authors have declared that no competing interests exist.
Concentrating on oncogenic role and increased plasma expression of microRNA(miR) 106b~25 clusters (involving miR 106b, miR 93 and miR 25), we evaluated significance of the over-expression of plasma miR 106b~25 in GC.
Methods Based on 65 pairs matched GC patients and health controls, we explored clinical significance of miR 106b~25 for GC and compared their diagnostic performance with conventional tumor biomarkers including CA724, CA242, CA199 and CEA.
Results Both miR 106b~25 cluster and conventional tumor biomarkers were significantly elevated in GC (All P1 in 55/65 GC patients, shown in S2 Table. The expression of miR 106b, miR 93 and miR 25 were significant higher in poor differentiated GC and well differentiated GC than control groups. What’s more, miR 106b, miR 93 and miR 25 expression were significant higher in poor differentiated GC patient than well differentiated GC patients. Meanwhile, the concentration of CA724, CA242, CA199 and CEA were significant higher than healthy controls either (all P
