RESEARCH ARTICLE
The Relationship between Dyslipidemia and Acute Axonal Function in Type 2 Diabetes Mellitus In Vivo Natalie C. G. Kwai1, William Nigole1, Ann M. Poynten2, Christopher Brown3, Arun V. Krishnan1*
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1 Prince of Wales Clinical School, University of New South Wales, Sydney, Australia, 2 Department of Endocrinology, Prince of Wales Hospital, Sydney, Australia, 3 National Health and Medical Research Council Clinical Trials Centre, University of Sydney, Sydney, Australia * [email protected]
Abstract OPEN ACCESS Citation: Kwai NCG, Nigole W, Poynten AM, Brown C, Krishnan AV (2016) The Relationship between Dyslipidemia and Acute Axonal Function in Type 2 Diabetes Mellitus In Vivo. PLoS ONE 11(4): e0153389. doi:10.1371/journal.pone.0153389 Editor: Christian Holscher, University of Lancaster, UNITED KINGDOM
Objectives Diabetic peripheral neuropathy (DPN) is a common and debilitating complication of diabetes mellitus. Treatment largely consists of symptom alleviation and there is a need to identify therapeutic targets for prevention and treatment of DPN. The objective of this study was to utilise novel neurophysiological techniques to investigate axonal function in patients with type 2 diabetes and to prospectively determine their relationship to serum lipids in type 2 diabetic patients.
Received: October 22, 2015
Methods
Accepted: March 29, 2016
Seventy-one patients with type 2 diabetes were consecutively recruited and tested. All patients underwent thorough clinical neurological assessments including nerve conduction studies, and median motor axonal excitability studies. Studies were also undertaken in age matched normal control subjects(n = 42). Biochemical studies, including serum lipid levels were obtained in all patients. Patient excitability data was compared to control data and linear regression analysis was performed to determine the relationship between serum triglycerides and low density lipoproteins and excitability parameters typically abnormal in type 2 diabetic patients.
Published: April 14, 2016 Copyright: © 2016 Kwai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: The authors are unable to make the data set publicly available due to ethical reasons surrounding dissemination of patient clinical details. However, interested investigators may make a request to obtain the data by contacting the corresponding author, Professor Arun Krishnan. Funding: NK was supported by an Australian Postgraduate Award scholarship from the Australian Government's Department of Education and Training, https://education.gov.au/australian-postgraduateawards. AK was supported by a Career Development Fellowship from the Australian Government's National Health and Medical Research Council of
Results Patient mean age was 64.2±2.3 years, mean glycosylated haemoglobin (HbA1c%) was 7.8±0.3%, mean triglyceride concentration was 1.6±0.1 mmol/L and mean cholesterol concentration was 4.1±0.2mmol/L. Compared to age matched controls, median motor axonal excitability studies indicated axonal dysfunction in type 2 diabetic patients as a whole (T2DM) and in a subgroup of the patients without DPN (T2DM-NN). These included reduced percentage threshold change during threshold electrotonus at 10–20ms depolarising currents (TEd10–20ms)(controls 68.4±0.8, T2DM63.9±0.8, T2DM-NN64.8±1.6%,P
The Relationship between Dyslipidemia and Acute Axonal Function in Type 2 Diabetes Mellitus In Vivo Natalie C. G. Kwai1, William Nigole1, Ann M. Poynten2, Christopher Brown3, Arun V. Krishnan1*
a11111
1 Prince of Wales Clinical School, University of New South Wales, Sydney, Australia, 2 Department of Endocrinology, Prince of Wales Hospital, Sydney, Australia, 3 National Health and Medical Research Council Clinical Trials Centre, University of Sydney, Sydney, Australia * [email protected]
Abstract OPEN ACCESS Citation: Kwai NCG, Nigole W, Poynten AM, Brown C, Krishnan AV (2016) The Relationship between Dyslipidemia and Acute Axonal Function in Type 2 Diabetes Mellitus In Vivo. PLoS ONE 11(4): e0153389. doi:10.1371/journal.pone.0153389 Editor: Christian Holscher, University of Lancaster, UNITED KINGDOM
Objectives Diabetic peripheral neuropathy (DPN) is a common and debilitating complication of diabetes mellitus. Treatment largely consists of symptom alleviation and there is a need to identify therapeutic targets for prevention and treatment of DPN. The objective of this study was to utilise novel neurophysiological techniques to investigate axonal function in patients with type 2 diabetes and to prospectively determine their relationship to serum lipids in type 2 diabetic patients.
Received: October 22, 2015
Methods
Accepted: March 29, 2016
Seventy-one patients with type 2 diabetes were consecutively recruited and tested. All patients underwent thorough clinical neurological assessments including nerve conduction studies, and median motor axonal excitability studies. Studies were also undertaken in age matched normal control subjects(n = 42). Biochemical studies, including serum lipid levels were obtained in all patients. Patient excitability data was compared to control data and linear regression analysis was performed to determine the relationship between serum triglycerides and low density lipoproteins and excitability parameters typically abnormal in type 2 diabetic patients.
Published: April 14, 2016 Copyright: © 2016 Kwai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: The authors are unable to make the data set publicly available due to ethical reasons surrounding dissemination of patient clinical details. However, interested investigators may make a request to obtain the data by contacting the corresponding author, Professor Arun Krishnan. Funding: NK was supported by an Australian Postgraduate Award scholarship from the Australian Government's Department of Education and Training, https://education.gov.au/australian-postgraduateawards. AK was supported by a Career Development Fellowship from the Australian Government's National Health and Medical Research Council of
Results Patient mean age was 64.2±2.3 years, mean glycosylated haemoglobin (HbA1c%) was 7.8±0.3%, mean triglyceride concentration was 1.6±0.1 mmol/L and mean cholesterol concentration was 4.1±0.2mmol/L. Compared to age matched controls, median motor axonal excitability studies indicated axonal dysfunction in type 2 diabetic patients as a whole (T2DM) and in a subgroup of the patients without DPN (T2DM-NN). These included reduced percentage threshold change during threshold electrotonus at 10–20ms depolarising currents (TEd10–20ms)(controls 68.4±0.8, T2DM63.9±0.8, T2DM-NN64.8±1.6%,P
