Nutrition and Cancer
ISSN: 0163-5581 (Print) 1532-7914 (Online) Journal homepage: http://www.tandfonline.com/loi/hnuc20
The influence of topical and systemic vitamin E on ultraviolet light‐induced skin damage in Hairless Mice Ian R. Record , Ivor E. Dreosti , Mary Konstantinopoulos & Reg A. Buckley To cite this article: Ian R. Record , Ivor E. Dreosti , Mary Konstantinopoulos & Reg A. Buckley (1991) The influence of topical and systemic vitamin E on ultraviolet light‐induced skin damage in Hairless Mice, Nutrition and Cancer, 16:3-4, 219-225, DOI: 10.1080/01635589109514160 To link to this article: http://dx.doi.org/10.1080/01635589109514160
Published online: 04 Aug 2009.
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Date: 29 October 2015, At: 03:27
The Influence of Topical and Systemic Vitamin E on Ultraviolet Light-Induced Skin Damage in Hairless Mice
Downloaded by [Universite Laval] at 03:27 29 October 2015
Ian R. Record, Ivor E. Dreosti, Mary Konstantinopoulos, and Reg A. Buckley
Abstract Hairless mice were fed diets containing different levels of vitamin E or received topical applications of the vitamin for three weeks before a single exposure equivalent to one minimal erythematous dose of ultraviolet light provided by an artificial sunlight source. Lipid peroxidation and suppression of incorporation of thymidine into DNA were used to estimate the degree of damage caused by the radiation. Restriction of dietary vitamin E had little effect on degree of epidermal lipid peroxidation or on thymidine incorporation into DNA. High dietary levels of the vitamin did not alter the degree of lipid peroxidation; however, the incorporation of thymidine was restored to levels comparable to those of unirradiated animals. Topical administration of a 1% solution of the vitamin in ethanol 1 or 24 hours before irradiation also restored thymidine incorporation and reduced the degree of lipid peroxidation. The results suggest that both dietary and topical vitamin E are effective in protecting the epidermis against some of the early damage induced by ultraviolet radiation. (Nutr Cancer 16, 219-225, 1991)
Introduction
Exposure of unprotected skin to sunlight or to artificial sources producing light of a similar spectrum results in erythema, induction of pigmentation, dermal tissue alterations, and production of actinic skin tumors in humans and animals. Biochemically, two of the early events following exposure to ultraviolet (UV) light are the induction of lipid peroxidation (1,2) and a suppression of replicative DNA synthesis (3) due to DNA damage, both of which appear to be related to UV-induced free radical generation within the cells (4). In the past, several groups have studied the potential protective effect of several antioxidants, including vitamin E, against UV exposure. De Rios and co-workers (5) found no protective effect of vitamin E alone against UV-induced erythema. Khettab and colleagues (2) reported that topical application of the vitamin diminished the production of lipid peroxides in the epidermis. In addition, Kaidby and Kligman (1) found the levels of The authors are affiliated with the Division of Human Nutrition, CSIRO, Adelaide 5000, South Australia.
Copyright © 1991, Lawrence Erlbaum Associates, Inc.
lipid peroxides in the skin of vitamin E-deficient rats to be greater than those of control animals, although there was only a modest increase after UV exposure. The purpose of the current study was to determine whether variations in dietary vitamin E levels could influence very early cellular responses after modest exposure to simulated solar radiation and to compare any such effects with topical applications of the vitamin.
Downloaded by [Universite Laval] at 03:27 29 October 2015
Methods and Materials
Female hairless mice (SKH-1), 7-10 weeks old, were used throughout the study. Experimental diets based on soy meal were prepared as described earlier (6) and formulated to contain
ISSN: 0163-5581 (Print) 1532-7914 (Online) Journal homepage: http://www.tandfonline.com/loi/hnuc20
The influence of topical and systemic vitamin E on ultraviolet light‐induced skin damage in Hairless Mice Ian R. Record , Ivor E. Dreosti , Mary Konstantinopoulos & Reg A. Buckley To cite this article: Ian R. Record , Ivor E. Dreosti , Mary Konstantinopoulos & Reg A. Buckley (1991) The influence of topical and systemic vitamin E on ultraviolet light‐induced skin damage in Hairless Mice, Nutrition and Cancer, 16:3-4, 219-225, DOI: 10.1080/01635589109514160 To link to this article: http://dx.doi.org/10.1080/01635589109514160
Published online: 04 Aug 2009.
Submit your article to this journal
Article views: 14
View related articles
Citing articles: 56 View citing articles
Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=hnuc20 Download by: [Universite Laval]
Date: 29 October 2015, At: 03:27
The Influence of Topical and Systemic Vitamin E on Ultraviolet Light-Induced Skin Damage in Hairless Mice
Downloaded by [Universite Laval] at 03:27 29 October 2015
Ian R. Record, Ivor E. Dreosti, Mary Konstantinopoulos, and Reg A. Buckley
Abstract Hairless mice were fed diets containing different levels of vitamin E or received topical applications of the vitamin for three weeks before a single exposure equivalent to one minimal erythematous dose of ultraviolet light provided by an artificial sunlight source. Lipid peroxidation and suppression of incorporation of thymidine into DNA were used to estimate the degree of damage caused by the radiation. Restriction of dietary vitamin E had little effect on degree of epidermal lipid peroxidation or on thymidine incorporation into DNA. High dietary levels of the vitamin did not alter the degree of lipid peroxidation; however, the incorporation of thymidine was restored to levels comparable to those of unirradiated animals. Topical administration of a 1% solution of the vitamin in ethanol 1 or 24 hours before irradiation also restored thymidine incorporation and reduced the degree of lipid peroxidation. The results suggest that both dietary and topical vitamin E are effective in protecting the epidermis against some of the early damage induced by ultraviolet radiation. (Nutr Cancer 16, 219-225, 1991)
Introduction
Exposure of unprotected skin to sunlight or to artificial sources producing light of a similar spectrum results in erythema, induction of pigmentation, dermal tissue alterations, and production of actinic skin tumors in humans and animals. Biochemically, two of the early events following exposure to ultraviolet (UV) light are the induction of lipid peroxidation (1,2) and a suppression of replicative DNA synthesis (3) due to DNA damage, both of which appear to be related to UV-induced free radical generation within the cells (4). In the past, several groups have studied the potential protective effect of several antioxidants, including vitamin E, against UV exposure. De Rios and co-workers (5) found no protective effect of vitamin E alone against UV-induced erythema. Khettab and colleagues (2) reported that topical application of the vitamin diminished the production of lipid peroxides in the epidermis. In addition, Kaidby and Kligman (1) found the levels of The authors are affiliated with the Division of Human Nutrition, CSIRO, Adelaide 5000, South Australia.
Copyright © 1991, Lawrence Erlbaum Associates, Inc.
lipid peroxides in the skin of vitamin E-deficient rats to be greater than those of control animals, although there was only a modest increase after UV exposure. The purpose of the current study was to determine whether variations in dietary vitamin E levels could influence very early cellular responses after modest exposure to simulated solar radiation and to compare any such effects with topical applications of the vitamin.
Downloaded by [Universite Laval] at 03:27 29 October 2015
Methods and Materials
Female hairless mice (SKH-1), 7-10 weeks old, were used throughout the study. Experimental diets based on soy meal were prepared as described earlier (6) and formulated to contain
