R ES E A RC H | R E PO R TS

RE FE RENCES AND N OT ES

1. G. F. Striedter, Principles of Brain Evolution (Sinauer Associates, Sunderland, MA, 2005). 2. P. Rakic, Nat. Rev. Neurosci. 10, 724–735 (2009). 3. J. H. Lui, D. V. Hansen, A. R. Kriegstein, Cell 146, 18–36 (2011). 4. V. Borrell, I. Reillo, Dev. Neurobiol. 72, 955–971 (2012). 5. M. Betizeau et al., Neuron 80, 442–457 (2013). 6. M. Florio, W. B. Huttner, Development 141, 2182–2194 (2014). 7. V. Borrell, M. Götz, Curr. Opin. Neurobiol. 27, 39–46 (2014). 8. T. Sun, R. F. Hevner, Nat. Rev. Neurosci. 15, 217–232 (2014). 9. E. Taverna, M. Götz, W. B. Huttner, Annu. Rev. Cell Dev. Biol. 30, 465–502 (2014). 10. I. H. Smart, C. Dehay, P. Giroud, M. Berland, H. Kennedy, Cereb. Cortex 12, 37–53 (2002). 11. E. Lewitus, I. Kelava, A. T. Kalinka, P. Tomancak, W. B. Huttner, PLOS Biol. 12, e1002000 (2014). 12. A. E. Ayoub et al., Proc. Natl. Acad. Sci. U.S.A. 108, 14950–14955 (2011). 13. S. A. Fietz et al., Proc. Natl. Acad. Sci. U.S.A. 109, 11836–11841 (2012). 14. M. L. Arcila et al., Neuron 81, 1255–1262 (2014). 15. J. A. Miller et al., Nature 508, 199–206 (2014). 16. Y. Arai et al., Nat. Commun. 2, 154 (2011). 17. A. Kawaguchi et al., Development 135, 3113–3124 (2008). 18. A. A. Pollen et al., Nat. Biotechnol. 32, 1053–1058 (2014). 19. J. H. Lui et al., Nature 515, 264–268 (2014). 20. S. A. Fietz, W. B. Huttner, Curr. Opin. Neurobiol. 21, 23–35 (2011). 21. A. Attardo, F. Calegari, W. Haubensak, M. Wilsch-Bräuninger, W. B. Huttner, PLOS ONE 3, e2388 (2008).

1470

27 MARCH 2015 • VOL 347 ISSUE 6229

22. S. L. Houlihan, Y. Feng, eLife 3, e03297 (2014). 23. A. Lukaszewicz et al., Neuron 47, 353–364 (2005). 24. X. Wang, J. W. Tsai, B. LaMonica, A. R. Kriegstein, Nat. Neurosci. 14, 555–561 (2011). 25. B. Riley, M. Williamson, D. Collier, H. Wilkie, A. Makoff, Genomics 79, 197–209 (2002). 26. F. Antonacci et al., Nat. Genet. 46, 1293–1302 (2014). 27. Y. Kagawa et al., PLOS ONE 8, e83629 (2013). 28. E. Zanin et al., Dev. Cell 26, 496–510 (2013). 29. P. H. Sudmant et al., Science 330, 641–646 (2010). 30. M. Meyer et al., Science 338, 222–226 (2012). 31. K. Prüfer et al., Nature 505, 43–49 (2014). 32. E. Taverna, C. Haffner, R. Pepperkok, W. B. Huttner, Nat. Neurosci. 15, 329–337 (2012). 33. R. Stahl et al., Cell 153, 535–549 (2013). 34. B. G. Rash, S. Tomasi, H. D. Lim, C. Y. Suh, F. M. Vaccarino, J. Neurosci. 33, 10802–10814 (2013). 35. M. B. Johnson et al., Nat. Neurosci. 10.1038/nn.3980 (2015). ACKN OWLED GMEN TS

We apologize to all researchers whose work could not be cited due to space limitation. We are grateful to the Services and Facilities of the MPI-CBG for the outstanding support provided, notably J. Helppi and his team of the Animal Facility, J. Peychl and his team of the Light Microscopy Facility, N. Lakshmanaperumal of the Bioinformatics Facility, and J. Jarrells and A. Eugster for support with single-cell analysis. We thank E. Perini for advice regarding RhoGAPs and all members of the Huttner lab for help

and discussion, especially D. Stenzel for support in obtaining fetal human tissue, J. Paridaen and M. Wilsch-Bräuninger for advice, and N. Kalebic and K. Long for critical reading of the manuscript. We thank B. Höber and A. Weihmann of MPI-EVA for expert DNA sequencing; B. Habermann of Max Planck Institute of Biochemistry (MPI-B) for bioinformatics advice; and K. Kaibuchi and M. Amano (Nagoya University) for pCAGGS-myc-KK1, pCAGGS-HA, and anti-MYPT1 antibody. M.F. was a member of the International Max Planck Research School for Cell, Developmental and Systems Biology and a doctoral student at the Technische Universität Dresden. W.B.H. was supported by grants from the Deutsche Forschungsgemeinschaft (DFG) (SFB 655, A2) and the European Research Council (250197), the DFG-funded Center for Regenerative Therapies Dresden, and the Fonds der Chemischen Industrie. The supplementary materials contain additional data. RNAseq raw data have been deposited with the Gene Expression Omnibus under accession codes GSE65000 and GSM1585634. SUPPLEMENTARY MATERIALS www.sciencemag.org/content/347/6229/1465/suppl/DC1

Materials and Methods Figs. S1 to S14 Tables S1 to S4 References (36–43) 30 October 2014; accepted 17 February 2015 Published online 26 February 2015; 10.1126/science.aaa1975

PARASITOLOGY

The in vivo dynamics of antigenic variation in Trypanosoma brucei Monica R. Mugnier, George A. M. Cross, F. Nina Papavasiliou* Trypanosoma brucei, a causative agent of African Sleeping Sickness, constantly changes its dense variant surface glycoprotein (VSG) coat to avoid elimination by the immune system of its mammalian host, using an extensive repertoire of dedicated genes. However, the dynamics of VSG expression in T. brucei during an infection are poorly understood. We have developed a method, based on de novo assembly of VSGs, for quantitatively examining the diversity of expressed VSGs in any population of trypanosomes and monitored VSG population dynamics in vivo. Our experiments revealed unexpected diversity within parasite populations and a mechanism for diversifying the genome-encoded VSG repertoire. The interaction between T. brucei and its host is substantially more dynamic and nuanced than previously expected.

T

he protozoan parasite Trypanosoma brucei, a major cause of human and animal Trypanosomiasis, lives extracellularly within its mammalian host, where it is constantly exposed to the host immune system. T. brucei has evolved a mechanism for antigenic variation during infection in which the parasite can turn on and off variant surface glycoprotein (VSG)–encoding genes from a genomic repertoire of ~2000 different genes (1). Each parasite expresses one VSG at a time, from one of ~15 telomeric expression sites (2); the rest (silent VSGs) sit in silent expression sites or in other genomic locations (1). The highly antigenic VSG is so densely packed on T. brucei’s surface that it obscures other cell-surface com1

Laboratory of Lymphocyte Biology, The Rockefeller University, New York, NY, USA. 2Laboratory of Molecular Parasitology, The Rockefeller University, New York, NY, USA.

*Corresponding author. E-mail: [email protected]

ponents from immune recognition. At any time, a few parasites in a population will stochastically switch their VSG. As previous variants are recognized by the immune system and cleared, newly switched variants emerge, giving rise to characteristic waves of parasitemia (3). These waves have long been interpreted as the sequential expression and clearance of one or a few VSGs, a notion supported by experimental evidence that relied on low-resolution approaches (4–8). Despite attempts at modeling, little is known about the kinetics of VSG expression during infection (9–12). To assess this, we developed a targeted RNA sequencing (RNA-seq) approach, termed VSG-seq, in which VSG cDNA, amplified by using conserved sequences at the 5′ and 3′ end of every mature VSG mRNA (fig. S1), is sequenced and then assembled de novo by a transcriptome reconstruction method called Trinity (13). We validated sciencemag.org SCIENCE

Downloaded from www.sciencemag.org on March 27, 2015

in neocortex folding at E18.5, reminiscent of gyrification, a hallmark of human neocortex (Fig. 4). Cortical plate area in the gyrus-like structures was increased compared with the contralateral smooth neocortex, with proper cortical lamination. The methodology for isolation of cortical progenitor subpopulations established here can be applied to other mammalian species, including primates, opening avenues for comparative evolutionary studies. Furthermore, the present transcriptome data provide insight into molecular differences between the various types of cortical NPCs in developing mouse and human neocortex and constitute a resource for future studies. A very recent, independent analysis of human radial glia transcriptome (19) has concentrated on genes present in both mouse and human genomes but expressed only in human cortical progenitors, identifying a role for platelet-derived growth factor signaling (16) in human radial glia. In contrast, we focus here on genes present only in the human, but not mouse, genome and highly expressed in basal radial glia. Thus, we identify ARHGAP11B as a humanspecific gene that amplifies basal progenitors and is capable of causing neocortex folding in mice (33, 34). This probably reflects a role for ARHGAP11B in development and evolutionary expansion of the human neocortex, a conclusion consistent with the finding that the gene duplication that created ARHGAP11B occurred on the human lineage after the divergence from the chimpanzee lineage but before the divergence from Neandertals, whose brain size was similar to that of modern humans. Note added in proof: In work published after online publication of this paper, Johnson et al. (35) used a complementary approach to similarly isolate and compare the transcriptomes of human and mouse apical and basal radial glia.

RE S EAR CH | R E P O R T S

this approach using mixtures of T. brucei cell lines expressing specific VSGs in known proportions (Fig. 1 and fig. S1). We compared measured expression of each VSG in the control populations with the known input and found that we could accurately assemble a VSG sequence expressed in as few as nine cells in the control mixture. VSG-seq is capable of reliably detecting variants present on 0.01% of parasites and quantifying a variant’s presence within the population, for variants present above 0.1% of the population (Fig. 1B and fig. S1). The apparent overestimation of minor VSGs in this control experiment is likely a result of low-level switching in the more abundant components of the mixture or low-level transcription of silent VSGs. The limits of detection and quantification for VSGseq appear to be independent of starting cell number because control mixtures made from 106 or 107 cells show similar results (Fig. 1B and fig. S1). To measure VSG expression within populations of T. brucei, we infected four mice with ~5 EATRO1125 parasites—originally expressing VSG AnTat1.1 (14, 15) but now heterogeneous

and each expressing a distinct VSG—and tracked VSG expression dynamics for 30 days. A few variants made up the majority of the population at each time, but surprisingly, each sample also contained many rare variants that would have been undetectable by using previous approaches (Fig. 2A and fig. S2). Infections showed great diversity even within parasitemic valleys. VSG-seq identified an average of 28 variants at each time point during the first 30 days of infection (Fig. 2C). One mouse (mouse 3) survived much longer than did the other three (106 days, compared with 41 to 72 days). VSG identity has not been

shown to affect growth rate or induction of the immune response (16, 17), so the increased survival and lower diversity in this mouse are more likely due to the polyclonal germline B cell repertoire, which is unique to each mouse (18), rather than the initiating VSG. Although in the later stages of this infection, VSG dynamics did appear qualitatively different—with variants persisting longer before clearance (Fig. 2B), possibly owing to immune system exhaustion— parasite populations remained diverse, with 30 to 66 variants detectable at each sampling (Fig. 2C).

% VSG CDS reconstructed

100

50

107 cells 106 cells

90 0 9, 00 0 90 ,0 0 90 0 0, 00 9, 00 0 0, 00 0

90

9

0

Calculated VSG expression (% of population)

No. of trypanosomes expressing VSG 100

Expected

10

106 cells 107 cells

1 0.1 0.01 0.001 0.0001

VS G 3 VS G 2 VS G 17 VS G 41 7 VS G 62 9 VS G 11 VS G 9

0.00001

Fig. 1. VSG-seq for assembly of VSGs and quantification VSG expression in a population of African trypanosomes. (A) Efficiency of VSG assembly (mean T SD). Control libraries made from a mixture of cell lines expressing different VSGs in known proportions were sequenced, and sequencing reads were assembled by using Trinity (13). Control mixtures were made from either 1 million or 10 million cells. (B) Quantification of VSG expression in control libraries (mean T SD). The black bar (“Expected”) represents the proportion of cells expressing that VSG in the control mixture, and the gray bars represent quantification for each library by use of VSG-seq.

SCIENCE sciencemag.org

Fig. 2. Complex dynamics throughout T. brucei infection. (A) Dynamics of VSG expression during early infection (days 6 to 30). Each colored line represents an individual VSG’s presence in the population, and the black line represents total parasitemia. Only variants present at >0.1% of the population at that time point are shown. When parasitemia could not be measured with a hemacytometer (0.01% of the population are included. 27 MARCH 2015 • VOL 347 ISSUE 6229

147 1

R ES E A RC H | R E PO R TS

To see whether these infections showed any bias or hierarchy in VSG expression (6, 8, 19, 20), we compared the VSG repertoires of all four mice. During the first 30 days of infection, 192 VSGs were expressed. Although each infection initiated with a different major VSG, the majority of variants (86%) appeared in more than one infection, and nearly half (46%) appeared in all four infections (Fig. 3C). Ninety-seven VSGs were expressed in mouse 3 from days 96 to 105. We compared the later occurring VSGs with those expressed early in mice 1, 2, and 4 and found none in common, even though early variants from mouse 3 also appeared frequently in mice 1, 2, and 4 (Fig. 3D).

Our experiments revealed striking diversity within each infection, but surprisingly frequent occurrence of the same VSGs in different infections. Within these diverse populations, many variants appeared transiently. We have termed these “minor” variants. By examining the fate of every variant, we found that at any time during the first 30 days of infection, about half (53%) of the variants present will never reach 1% of the population (Fig. 3A). Of the 48 VSGs that appeared in all four infections, few were consistently dominant, and few were only ever expressed as a minor variant (Fig. 3B). This implies that variant success is not determined only by the expressed

VSG. Instead, variant success is likely to be determined by interactions between the parasite and the humoral immune response in each animal. Because of antigenic similarity among some VSGs and their consequent elimination by crossreacting antibodies, the effective VSG repertoire will be smaller than the repertoire that the genome is capable of generating. Besides losing variants to cross-reactivity, T. brucei’s genomic VSG repertoire consists of a high proportion of incomplete VSG genes or pseudogenes (1, 21). Indeed, the 289 VSGs observed in our infections may represent more than half of the complete VSG repertoire [~400

Fig. 3. Variant emergence during infection. (A) Minor variants present at each time point (mean T SD). A minor variant is arbitrarily defined as any VSG that never exceeds 1% of the population during the course of infection in a single mouse. Major variants are any variant that exceeds 1% of the population at some point during infection. (B) Venn diagram comparing the fates of VSGs appearing in all four infections. (C) Intersection of sets of VSGs expressed during early infection (days 6 to 30). The total number of VSGs is listed in parentheses below the mouse number. (D) Venn diagrams showing intersection of VSGs expressed early in infection (VSGs from mouse 1, 2, or 4 versus VSGs from mouse 3, days 7 to 30) and intersection of VSGs expressed early in infection with VSGs expressed late in infection (VSGs from mouse 1, 2, or 4 versus VSGs from mouse 3, days 96 to 105).

Fig. 4. Mosaic VSGs can be identified throughout infection. (A) Transient expression of a mosaic VSG in the population. PCR confirmation of the mosaic is shown below. The black line represents total parasitemia at each day after infection, and the green line represents the number of parasites expressing the mosaic VSG. “n.q.” indicates that the VSG is detectable within the population, but not quantifiable. “n.d.” indicates that the VSG is not detectable within the population. Below the graph are products from PCR of gDNA at each time point, by using either primers specific for the mosaic VSG or the control gene, ura3. This VSG could not be amplified when first detected with VSG-seq, likely because of low cell numbers in the DNA sample (probably less than 10 cells). (B) Mosaic from late infection, with PCR confirmation of the mosaic shown below.

1472

27 MARCH 2015 • VOL 347 ISSUE 6229

sciencemag.org SCIENCE

RE S EAR CH | R E P O R T S

complete and predicted to be functional VSGs for the Lister427 strain (1), although the VSG repertoire for the EATRO1125 strain has not been fully elucidated]. The 65 to 135 VSGs observed before day 30 could represent up to 35% of the preexisting repertoire. Given the sampling frequency in our experiment, these values almost certainly underestimate the expressed VSG diversity in vivo. Therefore, much of the intact VSG repertoire is likely to have been expended early in an infection, as a result of expression and subsequent recognition by the immune system. As a result, the preexisting repertoire of complete VSGs would appear to be insufficient to support the sometimes years-long infections observed in the field. Although parasitemia is much lower in natural hosts, preexisting immunity is common in native mammals (22), requiring constant VSG diversification to sustain infection. Segmental gene conversion events have been demonstrated in both Trypanosoma equiperdum and T. brucei infections (7, 23, 24) generating “mosaic” VSGs that were not previously encoded in the genome. Previous studies had noted that mosaics tend to arise later in infection but have not determined when these variants are formed within the genome, or how. It is unknown whether mosaic VSGs form at the active expression site or within the silent repertoire before expression. To identify possible mosaics, we compared expressed VSG sequences to two independently assembled genomes for this parasite strain. Because of limitations in the amount of material available at each time point, we could choose only a few candidates for validation. To test that these were true mosaics and to determine when they formed within the genome, we used VSG-specific primers to confirm their absence from the genome of the parental strain and presence within genomic DNA (gDNA) collected during infection. We identified three mosaic VSGs using this approach. In each case, the mosaic VSG was only detectable by means of polymerase chain reaction (PCR) when it was also being expressed within the parasite population. This suggests that mosaic formation occurs, at least in these cases, shortly before expression, with subsequent transposition into the active expression site, or directly within the active expression site (Fig. 4 and fig. S3). Mosaic formation may be a mechanism for increasing repertoire diversity as infection progresses. Our results indicate that VSG switching does not occur at a rate that we would have expected to be just sufficient for immune evasion, with only a few variants present at any time. This suggests that recombinatorial mechanisms that expand the preexisting VSG repertoire may be critical for sustaining the long infections observed in natural hosts. Recent work on samples collected from sleeping sickness patients shows higherthan-expected VSG diversity (25), indicating that complex VSG dynamics are likely to be clinically relevant. Our results provide a foundation for the study of VSG switching and diversification in vivo and demonstrate the potential of highthroughput approaches for studying antigenic SCIENCE sciencemag.org

variation, in trypanosomes and other parasitic diseases, in naturally infected humans and animals. RE FERENCES AND NOTES 1. G. A. M. Cross, H. S. Kim, B. Wickstead, Mol. Biochem. Parasitol. 195, 59–73 (2014). 2. C. Hertz-Fowler et al., PLOS ONE 3, e3527 (2008). 3. R. Ross, D. Thomson, Proc. R. Soc. London Ser. B 82, 411–415 (1910). 4. C. M. Turner, J. D. Barry, Parasitology 99, 67–75 (1989). 5. C. M. Turner, FEMS Microbiol. Lett. 153, 227–231 (1997). 6. E. N. Miller, M. J. Turner, Parasitology 82, 63–80 (1981). 7. J. P. J. Hall, H. Wang, J. D. Barry, PLOS Pathog. 9, e1003502 (2013). 8. L. J. Morrison, P. Majiwa, A. F. Read, J. D. Barry, Int. J. Parasitol. 35, 961–972 (2005). 9. K. A. Lythgoe, L. J. Morrison, A. F. Read, J. D. Barry, Proc. Natl. Acad. Sci. U.S.A. 104, 8095–8100 (2007). 10. P. MacGregor, N. J. Savill, D. Hall, K. R. Matthews, Cell Host Microbe 9, 310–318 (2011). 11. S. A. Frank, Proc. Biol. Sci. 266, 1397–1401 (1999). 12. E. Gjini, D. T. Haydon, J. D. Barry, C. A. Cobbold, Proc. Biol. Sci. 280, 20122129–20122129 (2013). 13. M. G. Grabherr et al., Nat. Biotechnol. 29, 644–652 (2011). 14. N. Van Meirvenne, P. G. Janssens, E. Magnus, Ann. Soc. Belg. Med. Trop. 55, 1–23 (1975). 15. F. Claes et al., PLOS Negl. Trop. Dis. 3, e486 (2009). 16. J. R. Seed, J. Protozool. 25, 526–529 (1978). 17. A. R. Gray, J. Gen. Microbiol. 41, 195–214 (1965). 18. J. Lu et al., Mol. Immunol. 57, 274–283 (2014). 19. P. J. Myler, A. L. Allen, N. Agabian, K. Stuart, Infect. Immun. 47, 684–690 (1985).

20. A. Y. Liu, P. A. Michels, A. Bernards, P. Borst, J. Mol. Biol. 182, 383–396 (1985). 21. M. Berriman et al., Science 309, 416–422 (2005). 22. L. Marcello, J. D. Barry, J. Eukaryot. Microbiol. 54, 14–17 (2007). 23. S. M. Kamper, A. F. Barbet, Mol. Biochem. Parasitol. 53, 33–44 (1992). 24. C. Roth, F. Bringaud, R. E. Layden, T. Baltz, H. Eisen, Proc. Natl. Acad. Sci. U.S.A. 86, 9375–9379 (1989). 25. B. A. Eyford, R. Ahmad, J. C. Enyaru, S. A. Carr, T. W. Pearson, PLOS ONE 8, e71463 (2013). AC KNOWLED GME NTS

We thank A. Ivens, K. Matthews, K. Gunasekera, and I. Roditi for generously sharing their independently assembled genome sequences and J. Scott for help with early optimization experiments. The work presented has been supported in part by the NIH/National Institute of Allergy and Infectious Diseases (AI085973) to F.N.P., by an NSF Graduate Research Fellowship (DGE-1325261) to M.R.M., and by a Rockefeller University Women in Science Fellowship to M.R.M. All raw data has been deposited to the National Center for Biotechnology Information’s Sequence Read Archive under accession number SRP051697, along with all the methods used to generate the figures. SUPPLEMENTARY MATERIALS

www.sciencemag.org/content/347/6229/1470/suppl/DC1 Materials and Methods Figs. S1 to S3 References (26–30) Databases S1 to S5 10 December 2014; accepted 19 February 2015 10.1126/science.aaa4502

GEOMICROBIOLOGY

Redox cycling of Fe(II) and Fe(III) in magnetite by Fe-metabolizing bacteria James M. Byrne,1*† Nicole Klueglein,1† Carolyn Pearce,2,3 Kevin M. Rosso,3 Erwin Appel,4 Andreas Kappler1 Microorganisms are a primary control on the redox-induced cycling of iron in the environment. Despite the ability of bacteria to grow using both Fe(II) and Fe(III) bound in solid-phase iron minerals, it is currently unknown whether changing environmental conditions enable the sharing of electrons in mixed-valent iron oxides between bacteria with different metabolisms. We show through magnetic and spectroscopic measurements that the phototrophic Fe(II)-oxidizing bacterium Rhodopseudomonas palustris TIE-1 oxidizes magnetite (Fe3O4) nanoparticles using light energy. This process is reversible in co-cultures by the anaerobic Fe(III)-reducing bacterium Geobacter sulfurreducens. These results demonstrate that Fe ions bound in the highly crystalline mineral magnetite are bioavailable as electron sinks and electron sources under varying environmental conditions, effectively rendering magnetite a naturally occurring battery.

I

ron is critical to all living organisms, with many bacteria having developed pathways to access iron either as a nutrient or as an electron acceptor or donor, depending on its mobility, oxidation state, and bioavailability (1). Fe(III)-reducing bacteria, including Geobacter sulfurreducens, combine reduction of Fe(III) with oxidation of organic matter or H2 for energy conservation (2), whereas pho-

totrophic Fe(II)-oxidizing bacteria such as Rhodopseudomonas palustris TIE-1 grow in light with Fe(II) or H2 as the electron donor (3). Bacteria of the Geobacter genus and photoferrotrophs have previously been shown to simultaneously occur in sediments (4, 5). The mixed-valent magnetic mineral magnetite (Fe3O4), which contains both Fe(II) and Fe(III) in a 1:2 ratio, is often a byproduct of these Fe-metabolization 27 MARCH 2015 • VOL 347 ISSUE 6229

1473

The in vivo dynamics of antigenic variation in Trypanosoma brucei Monica R. Mugnier et al. Science 347, 1470 (2015); DOI: 10.1126/science.aaa4502

If you wish to distribute this article to others, you can order high-quality copies for your colleagues, clients, or customers by clicking here. Permission to republish or repurpose articles or portions of articles can be obtained by following the guidelines here. The following resources related to this article are available online at www.sciencemag.org (this information is current as of March 26, 2015 ): Updated information and services, including high-resolution figures, can be found in the online version of this article at: http://www.sciencemag.org/content/347/6229/1470.full.html Supporting Online Material can be found at: http://www.sciencemag.org/content/suppl/2015/03/25/347.6229.1470.DC1.html This article cites 30 articles, 11 of which can be accessed free: http://www.sciencemag.org/content/347/6229/1470.full.html#ref-list-1 This article appears in the following subject collections: Microbiology http://www.sciencemag.org/cgi/collection/microbio

Science (print ISSN 0036-8075; online ISSN 1095-9203) is published weekly, except the last week in December, by the American Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005. Copyright 2015 by the American Association for the Advancement of Science; all rights reserved. The title Science is a registered trademark of AAAS.

Downloaded from www.sciencemag.org on March 27, 2015

This copy is for your personal, non-commercial use only.

The in vivo dynamics of antigenic variation in Trypanosoma brucei.

Trypanosoma brucei, a causative agent of African Sleeping Sickness, constantly changes its dense variant surface glycoprotein (VSG) coat to avoid elim...
997KB Sizes 8 Downloads 11 Views