J Infect Chemother xxx (2015) 1e4
Contents lists available at ScienceDirect
Journal of Infection and Chemotherapy journal homepage: http://www.elsevier.com/locate/jic
Original article
The IgG avidity value for the prediction of Toxoplasma gondii infection in the amniotic fluid Kenji Tanimura a, Akira Nishikawa b, Shinya Tairaku a, Nanae Shinozaki a, Masashi Deguchi a, Mayumi Morizane a, Yasuhiko Ebina a, Ichiro Morioka c, Hideto Yamada a, * a b c
Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe, Japan NTT East Sapporo Medical Center, Sapporo, Japan Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe, Japan
a r t i c l e i n f o
a b s t r a c t
Article history: Received 18 March 2015 Received in revised form 8 May 2015 Accepted 29 May 2015 Available online xxx
Primary Toxoplasma gondii (T. gondii) infection during pregnancy may lead to congenital toxoplasmosis. Maternal screening using T. gondii IgG avidity measurement and multiplex nested PCR was performed. The aim of this prospective cohort study was to determine a cut-off value of IgG avidity index (AI) for the prediction of the presence of T. gondii DNA in the amniotic fluid. One hundred thirty-nine women with positive or equivocal tests for IgM underwent both serum IgG avidity measurement and PCR analysis for the amniotic fluid. Nine had positive PCR results, and three of them were diagnosed as having congenital infection. A cut-off value of IgG AI was determined using receiver operating characteristic analysis. IgG AI (mean 13%) in women with positive PCR results was significantly lower than that (39%) in women with negative results. A cut-off value of
Contents lists available at ScienceDirect
Journal of Infection and Chemotherapy journal homepage: http://www.elsevier.com/locate/jic
Original article
The IgG avidity value for the prediction of Toxoplasma gondii infection in the amniotic fluid Kenji Tanimura a, Akira Nishikawa b, Shinya Tairaku a, Nanae Shinozaki a, Masashi Deguchi a, Mayumi Morizane a, Yasuhiko Ebina a, Ichiro Morioka c, Hideto Yamada a, * a b c
Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe, Japan NTT East Sapporo Medical Center, Sapporo, Japan Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe, Japan
a r t i c l e i n f o
a b s t r a c t
Article history: Received 18 March 2015 Received in revised form 8 May 2015 Accepted 29 May 2015 Available online xxx
Primary Toxoplasma gondii (T. gondii) infection during pregnancy may lead to congenital toxoplasmosis. Maternal screening using T. gondii IgG avidity measurement and multiplex nested PCR was performed. The aim of this prospective cohort study was to determine a cut-off value of IgG avidity index (AI) for the prediction of the presence of T. gondii DNA in the amniotic fluid. One hundred thirty-nine women with positive or equivocal tests for IgM underwent both serum IgG avidity measurement and PCR analysis for the amniotic fluid. Nine had positive PCR results, and three of them were diagnosed as having congenital infection. A cut-off value of IgG AI was determined using receiver operating characteristic analysis. IgG AI (mean 13%) in women with positive PCR results was significantly lower than that (39%) in women with negative results. A cut-off value of
