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The Galvanotactic Migration of Keratinocytes is Enhanced by Hypoxic Preconditioning

received: 24 October 2014 accepted: 09 April 2015 Published: 19 May 2015

Xiaowei Guo, Xupin Jiang, Xi Ren, Huanbo Sun, Dongxia Zhang, Qiong Zhang, Jiaping Zhang & Yuesheng Huang The endogenous electric field (EF)-directed migration of keratinocytes (galvanotaxis) into wounds is an essential step in wound re-epithelialization. Hypoxia, which occurs immediately after injury, acts as an early stimulus to initiate the healing process; however, the mechanisms for this effect, remain elusive. We show here that the galvanotactic migration of keratinocytes was enhanced by hypoxia preconditioning as a result of the increased directionality rather than the increased motility of keratinocytes. This enhancement was both oxygen tension- and preconditioning time-dependent, with the maximum effects achieved using 2% O2 preconditioning for 6 hours. Hypoxic preconditioning (2% O2, 6 hours) decreased the threshold voltage of galvanotaxis to 

The Galvanotactic Migration of Keratinocytes is Enhanced by Hypoxic Preconditioning.

The endogenous electric field (EF)-directed migration of keratinocytes (galvanotaxis) into wounds is an essential step in wound re-epithelialization. ...
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