J Physiol Biochem DOI 10.1007/s13105-014-0317-4
ORIGINAL PAPER
The effects of resveratrol on tissue injury, oxidative damage, and pro-inflammatory cytokines in an experimental model of acute pancreatitis Ahmet Gulcubuk & Damla Haktanir & Aris Cakiris & Duran Ustek & Ozlem Guzel & Muzaffer Erturk & Funda Yildirim & Ibrahim Akyazi & Hayriye Cicekci & Mehmet Hanifi Durak & Sema Sandikci-Altunatmaz & Kemal Altunatmaz
Received: 16 May 2013 / Accepted: 22 January 2014 # University of Navarra 2014
Abstract Acute pancreatitis (AP) is an acute inflammatory condition that results from the digestion of pancreatic tissue by its own enzymes released from the acinar cells. The objective of this study was to investigate the effects of resveratrol on oxidative damage, proinflammatory cytokines, and tissue injury involved with AP induced in a rat model using sodium taurocholate (n=60). There were three treatment groups with 20 rats per group. Groups I and II received 3 % sodium taurocholate solution, while group III underwent the same surgical procedure yet did not receive sodium taurocholate. In addition, group II received 30 mg/kg resveratrol solution. Rats were sacrificed at 2, 6, 12, and 24 h time points following the induction of AP. Blood and pancreatic tissue samples were collected and subjected to biochemical assays, Western blot assays, and A. Gulcubuk (*) : D. Haktanir : F. Yildirim Department of Pathology, Faculty of Veterinary Medicine, Istanbul University, 34320 Avcilar, Istanbul, Turkey e-mail: [email protected] A. Cakiris : D. Ustek Department of Genetics, The Research Institute for Experimental Medicine, Istanbul University, 34093 Istanbul, Turkey O. Guzel : K. Altunatmaz Department of Surgery, Faculty of Veterinary Medicine, Istanbul University, 34320 Avcilar, Istanbul, Turkey M. Erturk Department of Gastroenterology, Gaziantep American Hospital, 27300 Gaziantep, Turkey
histopathologic evaluations. Resveratrol did not reduce trypsin levels and prevent tissue damage. Resveratrol prevented IκB degradation (except for 6 h) and decreased nuclear factor-κB (NF-κB), activator protein-1 (AP-1) (except for 24 h), and levels of TNF-α, IL-6 (except for 24 h), and iNOS in the pancreatic tissue at all time points (P
ORIGINAL PAPER
The effects of resveratrol on tissue injury, oxidative damage, and pro-inflammatory cytokines in an experimental model of acute pancreatitis Ahmet Gulcubuk & Damla Haktanir & Aris Cakiris & Duran Ustek & Ozlem Guzel & Muzaffer Erturk & Funda Yildirim & Ibrahim Akyazi & Hayriye Cicekci & Mehmet Hanifi Durak & Sema Sandikci-Altunatmaz & Kemal Altunatmaz
Received: 16 May 2013 / Accepted: 22 January 2014 # University of Navarra 2014
Abstract Acute pancreatitis (AP) is an acute inflammatory condition that results from the digestion of pancreatic tissue by its own enzymes released from the acinar cells. The objective of this study was to investigate the effects of resveratrol on oxidative damage, proinflammatory cytokines, and tissue injury involved with AP induced in a rat model using sodium taurocholate (n=60). There were three treatment groups with 20 rats per group. Groups I and II received 3 % sodium taurocholate solution, while group III underwent the same surgical procedure yet did not receive sodium taurocholate. In addition, group II received 30 mg/kg resveratrol solution. Rats were sacrificed at 2, 6, 12, and 24 h time points following the induction of AP. Blood and pancreatic tissue samples were collected and subjected to biochemical assays, Western blot assays, and A. Gulcubuk (*) : D. Haktanir : F. Yildirim Department of Pathology, Faculty of Veterinary Medicine, Istanbul University, 34320 Avcilar, Istanbul, Turkey e-mail: [email protected] A. Cakiris : D. Ustek Department of Genetics, The Research Institute for Experimental Medicine, Istanbul University, 34093 Istanbul, Turkey O. Guzel : K. Altunatmaz Department of Surgery, Faculty of Veterinary Medicine, Istanbul University, 34320 Avcilar, Istanbul, Turkey M. Erturk Department of Gastroenterology, Gaziantep American Hospital, 27300 Gaziantep, Turkey
histopathologic evaluations. Resveratrol did not reduce trypsin levels and prevent tissue damage. Resveratrol prevented IκB degradation (except for 6 h) and decreased nuclear factor-κB (NF-κB), activator protein-1 (AP-1) (except for 24 h), and levels of TNF-α, IL-6 (except for 24 h), and iNOS in the pancreatic tissue at all time points (P
