The effects of capture, "stress," and storage of whole blood on the red blood cells, plasma proteins, glucose, and electrolytes of the winter flounder (Pseudopleuronectes americanus) G. L. FLETCHER

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Marine Sciences Research Laboratory, Memorial Universify of Newfoundland, St. John's, Newfoundland Received July 2, 1974 FLETCHER, G. L. 1975. The effects of capture, "stress," and storage of whole blood on the red blood cells, plasma proteins, glucose, and electrolytes of the winter flounder (Pseudopleuronectes americanus). Can. J. Zool. 53: 197-206. When the blood from flounder caught in the field was compared with the blood of laboratorymaintained flounder, a number of differences were observed. Hematocrits, mean red blood cell volumes, blood hemoglobin, plasma osmolality, and plasma Na+ and C1- were significantly higher in the field flounder, while the mean cell hemoglobin concentrations and plasma Kt were significantly lower. Experiments conducted on the effects of "stress" suggested that differences in plasma osmolality, protein, NaC,CI-, red blood cells, hemoglobin, and mean cell hemoglobin between field and laboratory flounder could have been due to the effects of capture on the field fish. The high hematocrits and mean cell volumes, and the low mean cell hemoglobin concentrations observed in the field samples may have been caused by the combined effects of capture and the 1.5- to 3-h storage of the red blood cells when they were transported back to the laboratory. The low K+ concentrations observed in the plasma of the field blood samples appeared to be due to the storage of the blood samples. F L E T C ~G. RL. , 195. The effects of capture, "stress," and s t o w of whole blood on the red blood cells. plasma proteins, glucose, amd elecwolytes of the winter flounder (Pscudople8rronectes nmericanus). Can. I. Zool. 53: 197-206. En cornparant le sang de flttans captunis cn mtr et le sang de ACtans tlevks en Iaboratoim.on constate un certainnornbre de diffhnces. L'hirnatocrite, le volume moyendesglobules rouges, I'himoglobine du sang. t'osmolilltti du plmma, les concentrations de Na* et de C1- du plasma sont s~gn~ficativernent plus C l e v k ~ches le poisson en milieu naturel, alors que les concentmtiuns rnoyennes d'htmoglob~necel!ularre et de K+du plasma sont signifiativement plus basses. Des exp5riences destintes attudier les effets du "s'lrers" demontrent que les differences dans I'osmolalit~du plasma, les concentmiions de protiines, de Na+ et de CI-, les glohule~rouges, 1'hCmoglobine et I'himogiobtne cellula~remoyenne entre le flttan en milieu naturel et le Retan ilevi en laboratoire peubent &re diis aux effets de la capture sur le flitan en milieu naturel. Les hematocrites e l les volumes cellulaires rnoyens

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53, 1975

TABLE 2 Changes in the blood of flounder transferred from the field to the laboratory Blood samples obtained from: field (Apr. 6,1972)

laboratory (Apr. 10, 1972)

Difference

P

k0.403 2.17 k0.155 114

k0.0255 2.14 k0.140 87.9

k0.368 -0.03 k0.142 -25.9

NS

Hematocrit,

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%

Hemoglobin. g / l o ml . RBC, x 106/mm3 MCV, p3

10.01

MCHC, g/100 ml Osmolality, mosm/kg Protein, g/100 ml NOTE:Eight winter flounder were caught by divers on April 6 1972. A I-ml blood sample was collected from each fish as soon after capture as possible. Each fish was tagged immhiately after the blood sampling. The fish were then transported to the laboratory, where, 4 days later, another blood sample was obtained. The temperature of the water at both sampling sites was about -l°C. RBC = red blood cells MCV = mean cell volume MCH = mean cell hemoglobin; MCHC = mean cell hemoglobin concentration; oskolality and protein concentdtions were measured In the plasma. All values are expressed as means k SE.

ally lower. However, there were times when one or the other of these parameters did not differ statistically between

The effects of capture, "stress," and storage of whole blood on the red blood cells, plasma proteins, glucose, and electrolytes of the winter flounder (Pseudopleuronectes americanus).

The effects of capture, "stress," and storage of whole blood on the red blood cells, plasma proteins, glucose, and electrolytes of the winter flounder...
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