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Asian-Aust. J. Anim. Sci. Vol. 26, No. 4 : 517-522 April 2013 http://dx.doi.org/10.5713/ajas.2012.12480
www.ajas.info pISSN 1011-2367 eISSN 1976-5517
The Effect of Plant Extracts on In-vitro Ruminal Fermentation, Methanogenesis and Methane-related Microbes in the Rumen E. T. Kim, K. -S. Min1, C. -H. Kim2, Y. H. Moon3, S. C. Kim4 and S. S. Lee* Division of Applied Life Science (BK21 program), Gyeongsang National University (Institute of Agriculture and Life Science), Jinju, 660-701, Korea ABSTRACT: The effect on methanogens attached to the surface of rumen ciliate protozoa by the addition of plant extracts (pine needles and ginkgo leaves) was studied with particular reference to their effectiveness for decreasing methane emission. The plant extracts (pine needles and ginkgo leaves) were added to an in vitro fermentation incubated with rumen fluid. The microbial population including bacteria, ciliated-associated methanogen, four different groups of methanogens and Fibrobacter succinogenes were quantified by using the real-time PCR. Gas profiles including methane, carbon dioxide and hydrogen, and runinal fermentation characteristics were observed in vitro. The methane emission from samples with an addition of individual juices from pine needles, ginkgo leaves and 70% ethanol extract from ginko leaves was significantly lower (p
Asian-Aust. J. Anim. Sci. Vol. 26, No. 4 : 517-522 April 2013 http://dx.doi.org/10.5713/ajas.2012.12480
www.ajas.info pISSN 1011-2367 eISSN 1976-5517
The Effect of Plant Extracts on In-vitro Ruminal Fermentation, Methanogenesis and Methane-related Microbes in the Rumen E. T. Kim, K. -S. Min1, C. -H. Kim2, Y. H. Moon3, S. C. Kim4 and S. S. Lee* Division of Applied Life Science (BK21 program), Gyeongsang National University (Institute of Agriculture and Life Science), Jinju, 660-701, Korea ABSTRACT: The effect on methanogens attached to the surface of rumen ciliate protozoa by the addition of plant extracts (pine needles and ginkgo leaves) was studied with particular reference to their effectiveness for decreasing methane emission. The plant extracts (pine needles and ginkgo leaves) were added to an in vitro fermentation incubated with rumen fluid. The microbial population including bacteria, ciliated-associated methanogen, four different groups of methanogens and Fibrobacter succinogenes were quantified by using the real-time PCR. Gas profiles including methane, carbon dioxide and hydrogen, and runinal fermentation characteristics were observed in vitro. The methane emission from samples with an addition of individual juices from pine needles, ginkgo leaves and 70% ethanol extract from ginko leaves was significantly lower (p
