JOURNAL OF INTERFERON RESEARCH 10:579-588 (1990)
Mary
Ann
Liebert, Inc., Publishers
The Effect of Interferon Treatment on 14 Human Colorectal Cancer Cell Lines: Growth and Carcinoembryonic Antigen Secretion In Vitro CAROL ANN TOTH and PETER THOMAS
ABSTRACT The effect of interferons (IFNs) on growth inhibition and carcinoembryonic antigen (CEA) secretion by 14 established human colorectal carcinoma cell lines was studied in vitro. The cell lines were characterized by morphologic differentiation, level of CEA production, and rate of growth. All cell lines were treated in vitro with recombinant human IFN (a, ß, and 7) and the effect of treatment on growth rate and CEA secretion determined. Each cell line exhibited an individual pattern of growth inhibition that was independent of degree of differentiation, level of CEA production, and rate of growth. IFN-ß treatment did not increase CEA secretion in any of the cell lines studied. IFN-a and IFN-7 resulted in increased CEA production (2- to 81-fold increase) primarily in the moderately to welldifferentiated cell lines. IFN-7 was a more potent inducer of enhanced CEA secretion than IFN-a. The more poorly differentiated cell lines did not produce CEA and could not be induced to do so by any of the IFNs.
INTRODUCTION
CARCINOMA patients 147,000
OF THE COLON AND RECTUM is the second leading cause of cancer death in the United States. In 1988 about new cases of colorectal cancer were diagnosed and 60,000 deaths reported.'" At least 50-60% of with colorectal cancer who have undergone surgery for removal of their primary tumor will develop metastatic disease. To date there is no effective standard adjuvant chemotherapy or immunotherapy for the high-risk patient after surgical removal of their primary tumor. oo i/-! co
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Mary
Ann
Liebert, Inc., Publishers
The Effect of Interferon Treatment on 14 Human Colorectal Cancer Cell Lines: Growth and Carcinoembryonic Antigen Secretion In Vitro CAROL ANN TOTH and PETER THOMAS
ABSTRACT The effect of interferons (IFNs) on growth inhibition and carcinoembryonic antigen (CEA) secretion by 14 established human colorectal carcinoma cell lines was studied in vitro. The cell lines were characterized by morphologic differentiation, level of CEA production, and rate of growth. All cell lines were treated in vitro with recombinant human IFN (a, ß, and 7) and the effect of treatment on growth rate and CEA secretion determined. Each cell line exhibited an individual pattern of growth inhibition that was independent of degree of differentiation, level of CEA production, and rate of growth. IFN-ß treatment did not increase CEA secretion in any of the cell lines studied. IFN-a and IFN-7 resulted in increased CEA production (2- to 81-fold increase) primarily in the moderately to welldifferentiated cell lines. IFN-7 was a more potent inducer of enhanced CEA secretion than IFN-a. The more poorly differentiated cell lines did not produce CEA and could not be induced to do so by any of the IFNs.
INTRODUCTION
CARCINOMA patients 147,000
OF THE COLON AND RECTUM is the second leading cause of cancer death in the United States. In 1988 about new cases of colorectal cancer were diagnosed and 60,000 deaths reported.'" At least 50-60% of with colorectal cancer who have undergone surgery for removal of their primary tumor will develop metastatic disease. To date there is no effective standard adjuvant chemotherapy or immunotherapy for the high-risk patient after surgical removal of their primary tumor. oo i/-! co
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