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Biochimica et Biophysica Acta, 471 ( 1 9 7 7 ) 1 0 5 - - 1 1 0 © E l s e v i e r / N o r t h - H o l l a n d B i o m e d i c a l Press

BBA 77842

THE EFFECT OF INORGANIC PHOSPHATE ON SODIUM FLUXES IN DOG RED BLOOD CELLS

P.R. M I L E S *, V. C A S T R A N O V A , L. B L E I G H a n d P. L E E

Appalachian Laboratory for Occupational Safety and Health, National Institute for Occupational Safety and Health, Department of Health, Education and Welfare, Morgantown, W. Va. 26505 and Department of Physiology and Biophysics, West Virginia University, Morgantown, W. Va. 26506 !U.S.A.) (Received M a r c h 3 0 t h , 1 9 7 7 )

Summary The effect of extracellular inorganic phosphate on Na ÷ movements in dog red blood cells has been studied. As the phosphate concentration is increased from 0 to 30 mM, Na ÷ efflux increases by 2- to 3-fold and Na* influx increases approximately 2-fold. This enhancement of Na ÷ fluxes by phosphate can be prevented by the addition of iodoacetate (1 mM), an inhibitor of glycolysis, or 4-acetamido-4'-iso-thiocyantostilbene-2,2'