Br. J. Cancer (1977) 35, 123

Letter to the Editor SIR,-We read with interest the letter by uptake by adriamycin is suggested, the Smith and Kundu (1976). Our work, using variance is too great for significance with a 3H-labelled adriamycin and digoxin, shows sample size of 4 animals. However, the inhibition of uptake of labelled adriamycin by results opposite to those reported. Cats of either sex were lightly anaesthe- digoxin is significant (P < 0 05) in the 6 tized, the chest rapidly opened, and the heart animals studied. Digoxin thus inhibits quickly removed and suspended from an adriamycin uptake acutely in the in vitro Anderson Heart Perfusion System. The com- Langendoff heart preparation. This obserposition of the perfusion solution was (g/l) vation combined with the initial work of NaCl 7-0, KCI 0-42, CaO2 0-24, MgCl2 0-2, Arena et al. (1972) that strophanthin may NaHCO3 2-1 and dextrose 1P8 with the inhibit myocardial adriamycin uptake, makes pH 735 and the temperature 38°C aerated the inhibition of adriamycin uptake by with 95% 02 and 5% CO2. The perfusion cardiac glycosides an area warranting further pressure was constant at 50 cmH2O and the study. heart rate was kept constant at 200 beats/min J. SOMBERG, N. CAGIN, H. BouNous, using ventricular pacing. B. LEVITT. Four groups were studied: [3H]digoxin New York Medical College, 625 mg/l, [3H]digoxin 625 mg/l + adriamycin Divisions of Pharmacology and Cardiology, 10 mg/I, [3H]adriamycin 10 mg/l, and [3H]- New York, N.Y., U.S.A. adriamycin 10 mg/l + digoxin 625 mg/l. The myocardial content of [3H]digoxin was 2-32 REFERENCES ± 0412 pmol/mg wet weight, while when adriamycin was infused, the labelled content ARENA, E., D'ALESSANDRA, N., DUSONCHET, L., GEBBIA, N., GERBASI, F., SANTGUEDOLCE, R. & of digoxin was 1-43 + 0 47 pmol/mg. RAUSA, L. (1972) Influence of Pharmacolsinetic [3H]adriamycin content was 0-069 + 0-01 Variations on the Pharmacological Properties of pmol/mg, and when adriamycin + digoxin Adramycin. International Symposium on Adramycin, Ed S. K. Carter. Berlin and New York: were infused simultaneously, the myocardial Springer-Verlag. p. 86. adriamycin content was reduced to 0-025 + SMITH, B. & KUNDU, D. (1976) Digoxin does not 0-01 pmol/mg. Prevent Daunorubicin or Adramycin from Binding Although the inhibition of labelled digoxin to Rat Heart Muscle. Br. J. Cancer, 33, 232.

The effect of digoxin on the binding of adriamycin to cat heart muscle.

Br. J. Cancer (1977) 35, 123 Letter to the Editor SIR,-We read with interest the letter by uptake by adriamycin is suggested, the Smith and Kundu (19...
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