The effect of aqueous extract of Xinjiang Artemisia rupestris L. (an influenza virus vaccine adjuvant) on enhancing immune responses and reducing antigen dose required for immunity Ailian Zhang1☯*, Danyang Wang1☯, Jinyao Li1, Feng Gao2, Xucheng Fan2
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1 Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, China, 2 Urumqi Center for Disease Control and Prevention, Urumqi, China ☯ These authors contributed equally to this work. * [email protected]
Abstract OPEN ACCESS Citation: Zhang A, Wang D, Li J, Gao F, Fan X (2017) The effect of aqueous extract of Xinjiang Artemisia rupestris L. (an influenza virus vaccine adjuvant) on enhancing immune responses and reducing antigen dose required for immunity. PLoS ONE 12(8): e0183720. https://doi.org/10.1371/ journal.pone.0183720 Editor: Farhat Afrin, Taibah University, SAUDI ARABIA Received: December 21, 2016 Accepted: August 9, 2017 Published: August 25, 2017 Copyright: © 2017 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper. Funding: This work was supported by the National Natural Science Foundation of China (31360224,31660259). The funders had no role in study design, data collection and analysis, decision to publish, preparation or of the manuscript.
Potent adjuvant can improve the effectiveness of vaccines and reduce the antigen doses required for initiating the protective immunity. In this study, we identified that aqueous extract of Artemisia rupestris L. (AEAR) could be employed as an efficient adjuvant for influenza virus vaccine (V) to enhance immune responses and reduce the antigen doses required for initiating immunity, without compromising the immune response. ICR mice were subcutaneously co-administrated with V combined with different concentrations of AEAR demonstrated that 300 μg AEAR could significantly improve hemagglutination inhibition (HI) and increase IgG antibody titers in serum (P