Mitochondrial DNA The Journal of DNA Mapping, Sequencing, and Analysis

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The complete mitochondrial genome of the predatory bug Orius sauteri (Poppius) (Hemiptera: Anthocoridae) Bao-Zhen Du, Fang-Fang Niu & Shu-Jun Wei To cite this article: Bao-Zhen Du, Fang-Fang Niu & Shu-Jun Wei (2014): The complete mitochondrial genome of the predatory bug Orius sauteri (Poppius) (Hemiptera: Anthocoridae), Mitochondrial DNA To link to this article: http://dx.doi.org/10.3109/19401736.2014.915537

Published online: 19 May 2014.

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Date: 06 November 2015, At: 03:50

http://informahealthcare.com/mdn ISSN: 1940-1736 (print), 1940-1744 (electronic) Mitochondrial DNA, Early Online: 1–2 ! 2014 Informa UK Ltd. DOI: 10.3109/19401736.2014.915537

MITOGENOME ANNOUNCEMENT

The complete mitochondrial genome of the predatory bug Orius sauteri (Poppius) (Hemiptera: Anthocoridae) Bao-Zhen Du1,2, Fang-Fang Niu1,3, and Shu-Jun Wei1

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1

Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China, 2Department of Plant Protection, Shandong Agricultural University, Tai’an, Shandong, China, and 3College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, China Abstract

Keywords

The complete mitochondrial genome of the predatory bug Orius sauteri (Poppius) (Hemiptera: Anthocoridae) was sequenced and analyzed (GenBank accession No. KJ671626). The length of this mitochondrial genome is 16,246 bp with 37 typical animal mitochondrial genes; i.e., 13 protein-coding, 2 rRNA and 22 tRNA genes, and an A+T-rich region. The gene arrangement is identical to that of the pupative ancestral arrangement of insects. All protein-coding genes start with ATN start codon, i.e. five ATAs, four ATTs, three ATGs and one ATC. Ten protein-coding genes stop with termination codon TAA. Three protein-coding genes use incomplete stop codon TA. The A+T-rich region are located between rrnS and trnM with a length of 1758 bp and an A+T content of 73.49%.

A+T-rich region, insect, mitochondrial genome

The predatory bug Orius sauteri (Poppius) (Hemiptera: Anthocoridae) is an important natural enemy that has been mass-reared for biological control of numerous pests in protected crop-production systems. Here, we determined the complete mitochondrial genome sequence of the O. sauteri (GenBank accession No. KJ671626) and consequently analyzed its general features. This is the second mitochondrial genome and first complete mitochondrial genome from the genus Orius (Hua et al., 2008). This mitochondrial genome was 16,246 bp long with the 37 typical animal mitochondrial genes, i.e. 13 protein-coding, 2 rRNA and 22 tRNA genes, and an A+T-rich region (Table 1). Gene arrangement of this mitochondrial genome is identical to the pupative ancestral arrangement of insects (Clary & Wolstenholme, 1985). The base composition of O. sauteri mitochondrial genome is normal to most insects (Liu & Liang, 2013; Li et al., 2012; Zhang et al., 2013). The A+T content of the entire mitochondrial genome was 65.68%, higher than G+C content. There were more A’s (43.49%) than T’s (33.19%) and more C’s (12.17%) than G’s (10.54%) on the majority strand, indicating a positive AT skew and negative GC skew (Wei et al., 2010a). There were totally 42 overlapped nucleotides between neighboring genes in 14 locations and the length of overlapped sequence is 1–8 bp, while there were totally 78 bp intergenic nucleotides in 10 locations and the length of intergenic spacer is 1–35 bp. Thirteen pairs of genes were directly adjacent without intergenetic or overlapping nucleotides.

Correspondence: Shu-Jun Wei, Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguanghuayuan Middle Road, Haidian District, Beijing 100097, China. Tel: +86 01051503439. Fax: +86 01051503899. E-mail: [email protected]

History Received 9 April 2014 Accepted 13 April 2014 Published online 19 May 2014

Table 1. Annotation of the complete mitochondrial genome of Orius sauteri.

Gene

Strand

trnI trnQ trnM nad2 trnW trnC trnY cox1 trnL2 cox2 trnK trnD atp8 atp6 cox3 trnG nad3 trnA trnR trnN trnS1 trnE trnF nad5 trnH nad4 nad4L trnT trnP nad6

+ – + + + – – + + + + + + + + + + + + + + + – – – – – + – +

Position 1–68 66–132 132–202 203–1171 1171–1236 1229–1291 1292–1357 1359–2897 2893–2958 2959–3602 3638–3708 3708–3773 3774–3929 3926–4593 4593–5378 5384–5446 5444–5800 5801–5862 5868–5932 5930–5994 5995–6061 6062–6125 6124–6189 6198–7886 7884–7947 7947–9278 9273–9556 9559–9623 9624–9690 9693–10,178

Intergenic Length Anti/ Stop nucleotides (bp) (bp) Start codon codon 68 67 71 969 66 63 66 1539 66 644 71 66 156 668 786 63 357 62 65 65 67 64 66 1689 64 1332 284 65 67 486

GAT TTG CAT ATA TCA GCA GTA ATG TAA ATT CTT GTC ATC ATA ATG TCC ATA TGC TCG GTT GCT TTC GAA ATT GTG ATG ATT TGT TGG ATT

TAA

TAA TAA TAA TA TAA TAA

TAA TAA TA TAA

–3 –1 0 –1 –8 0 1 –5 0 35 –1 0 –4 –1 5 –3 0 5 –3 0 0 –2 8 –3 –1 –6 2 0 2 5 (continued )

2

B.-Z. Du et al.

Mitochondrial DNA, Early Online: 1–2

A+T-rich region of other insects were not present in this species (Wei et al., 2010b, Zhang & Hewitt, 1997).

Table 1. Continued

Gene

Strand

Position

cob trnS2 nad1 trnL1 rrnL trnV rrnS AT

+ + – – – – –

10,184–11,314 11,318–11,387 11,405–12,321 12,322–12,387 12,388–13,633 13,634–13,701 13,702–14,488 14,489–16,246

Intergenic Length Anti/ Stop nucleotides (bp) (bp) Start codon codon 1131 70 917 66 1246 68 787 1758

ATA TGA ATA TAG AAT TAC AAA

TAA TA

3 17 0 0 0 0 0

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+ indicates that the gene is coded on the majority strand, while – indicates that the gene is coded on the minority strand. AT indicates the A+T-rich region.

The size of the protein-coding genes in O. sauteri mitochondrial genome is similar to their corresponding orthologs in other insects (Liu & Liang, 2013; Li et al., 2012; Zhang et al., 2013). The average A+T content of protein-coding genes is 75.9%. The lowest A+T content is 68.9% in the cox2, while the highest is 81.9% in nad2. All protein-coding genes start with ATN start codon, i.e. five ATAs, four ATTs, three ATGs and one ATC. Ten protein-coding genes stop with termination codon TAA. Three protein-coding genes used incomplete stop codon TA, which is commonly reported in other invertebrates (Masta & Boore, 2004). The length of the 22 tRNA genes ranges from 62 to 71 bp. The rrnL is 1246 bp long with an A+T content of 82.58% while the rrnS is 787 bp longh with an A+T content of 79.42%. The A+T-region was located between rrnS and trnM with a length of 1758 bp. The A+T content of this region was 73.49%, lower than that of the entire genome as in other heteropteran species (Hua et al., 2008). This region is believed to be involved in the regulation of transcription and control of DNA replication (Zhang & Hewitt, 1997). Repeat sequences with both large (106 bp) and samll (41 bp) repeat units are present in the A+T-rich region. However, conserved elements found in the

Declaration of interest The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper. Funding for this study was provided jointly by the 973 Program (2013CB127600), National Science Foundation of China (31101661), Beijing New Star Program on Science and Technology (2010B027) and Beijing Excellent Talents Program (2010D002020000010).

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The complete mitochondrial genome of the predatory bug Orius sauteri (Poppius) (Hemiptera: Anthocoridae).

The complete mitochondrial genome of the predatory bug Orius sauteri (Poppius) (Hemiptera: Anthocoridae) was sequenced and analyzed (GenBank accession...
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