Mitochondrial DNA The Journal of DNA Mapping, Sequencing, and Analysis

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The complete mitochondrial genome of an assassin bug Peirates arcuatus (Hemiptera: Reduviidae) Guangyu Zhao, Hu Li, Fan Song & Wanzhi Cai To cite this article: Guangyu Zhao, Hu Li, Fan Song & Wanzhi Cai (2014): The complete mitochondrial genome of an assassin bug Peirates arcuatus (Hemiptera: Reduviidae), Mitochondrial DNA To link to this article: http://dx.doi.org/10.3109/19401736.2013.867438

Published online: 17 Jan 2014.

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Date: 29 September 2015, At: 20:28

http://informahealthcare.com/mdn ISSN: 1940-1736 (print), 1940-1744 (electronic) Mitochondrial DNA, Early Online: 1–2 ! 2014 Informa UK Ltd. DOI: 10.3109/19401736.2013.867438

MITOGENOME ANNOUNCEMENT

The complete mitochondrial genome of an assassin bug Peirates arcuatus (Hemiptera: Reduviidae) Guangyu Zhao1, Hu Li1,2, Fan Song1, and Wanzhi Cai1 Department of Entomology and 2Department of Ornamental Horticulture, China Agricultural University, Beijing, China

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Abstract

Keywords

The complete mitochondrial genome of Peirates arcuatus (Sta˚l) is a typical double-stranded circular molecule 16,176 bp long with 37 genes usually present in animal mitochondrial genomes and a control region. Gene order is identical to that of the putative ancestral arrangement of insects and other assassin bugs. Thirteen protein-coding genes initiate with ATN codons and mostly terminate with TAN codons except for COII and COIII use a single T residue as the termination codon. All tRNA sequences can be folded into classic clover-leaf secondary structure except that the dihydrouridine (DHU) arm of tRNASer(AGN) forms a simple loop, and their length range from 63 to 69 bp. The control region is 1552 bp long and includes two complete 118-bp tandem repeats and one partial copy of anterior repeat unit.

Hemiptera, mitochondrial genome, reduviidae

Subfamily Peiratinae of the assassin bugs (Reduviidae) is huge and is distributed worldwide. Over 30 genera and 350 species have been described so far (Cai & Taylor, 2006). Species in this subfamily are primarily ground-dwelling and feed on other arthropods (Schuh & Slater, 1995). To date, the complete mitochondrial genomes of five assassin bugs from five subfamilies have been sequenced (Dotson & Beard, 2001; Gao et al., 2013; Hua et al., 2008; Li et al., 2011, 2013). In this study, we report the complete mitochondrial genome of Peirates arcuatus, the first representation of genus Peirates from Peiratinae. This mitochondrial genome is 16,176 bp long (GenBank accession number KF752445). It includes the entire set of 37 genes (i.e. 13 protein-coding genes, 22 tRNA genes and 2 rRNA genes) usually present in animal mitochondrial genomes and a control region (Figure 1). Gene order is identical to that of the putative ancestral arrangement of insects and other assassin bugs (Boore, 1999; Gao et al., 2013; Li et al., 2011, 2013). The only two inter-genic regions410 bp in length are between tRNAGln and tRNAMet, and between tRNASer(UCN) and ND1, the mtTERM binding site (Cameron & Whiting, 2008). There are a total of 74 overlapped nucleotides between genes in 12 locations, ranging from 1 to 22 bp in size. The nucleotide composition of the whole mitochondrial genome is significantly biased toward A þ T (71.3%), and displays positive AT- and negative GC-skews (0.128 and 0.176, respectively). Thirteen protein-coding genes are initiated with ATN as start codons (one with ATA, three with ATT, eight with ATG and one with ATC). Most coding sequences end with the stop codons TAA or TAG (10 with TAA and one with TAG)

History Received 4 November 2013 Revised 11 November 2013 Accepted 16 November 2013 Published online 17 January 2014

except for COII and COIII terminate with a single T residue adjacent to a downstream tRNA gene. Using a single T as stop codon is common in insect mitochondrial genomes (Li et al., 2012a,b). The mtDNA of P. arcuatus contains all set of 22 tRNA genes typically present in animal mitochondrial genomes, ranging from 63 to 70 bp in size. Among all tRNA genes, only tRNASer(AGN) cannot exhibit the classic clover-leaf secondary structure, due to the deficiency of the dihydrouridine (DHU) arm. According to the secondary structure, aberrant loop and non-Watson–Crick matches are found in some P. arcuatus tRNA genes, with the example of tRNASer(AGN), which possesses a long optimal base pairing (9 bp in contrast to the normal 5 bp) and a bulged nucleotide in the middle of the anticodon arm. The control region is located at the conserved position between srRNA and tRNAIle and is 1552 bp in length. It can be divided into five parts: a 422-bp leading sequence adjacent to srRNA, a string of 11 Gs, a 486-bp region overwhelmingly biased toward A þ T (76.1%), a region composed of two 118-bp complete tandem repeats and a partial copy of anterior repeat unit, and the remainder of the control region.

Declaration of interest Funding for this study was supported by grants from the National Basic Research Program of China (No. 2013CB127600), the National Natural Science Foundation of China (No. 31372229) and the Special Fund for Scientific Research (No. 2012FY111100) for WZC; and the China Postdoctoral Science Foundation (No. 2013M540167) and the Fundamental Research Funds for the Central Universities (2014BH021)

Correspondence: Dr. Wanzhi Cai, Department of Entomology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China. Tel: 86-10-62732885. Fax: 86-10-62732885. E-mail: [email protected]

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G. Zhao et al.

Mitochondrial DNA, Early Online: 1–2

Figure 1. Map of the mitochondrial genome of P. arcuatus. Direction of gene transcription is indicated by arrows. PCGs are shown as blue arrows, rRNAs as purple arrows, tRNAs as red arrows and large NC regions (4100 bp) as grey arrows. tRNAs are labeled according to single-letter IUPAC-IUB abbreviations (L1: CUN; L2: UUR; S1: AGN; S2: UCN). The GC content is plotted using a black sliding window, as the deviation from the average GC content of the entire sequence. GC-skew is plotted as the deviation from the average GC-skew of the entire sequence. Ticks in the inner cycle indicate the sequence length. for HL. All authors have read and approved the final manuscript. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

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The complete mitochondrial genome of an assassin bug Peirates arcuatus (Hemiptera: Reduviidae).

The complete mitochondrial genome of Peirates arcuatus (Stål) is a typical double-stranded circular molecule 16,176 bp long with 37 genes usually pres...
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