Chromosome Res DOI 10.1007/s10577-014-9457-1

REVIEW

TALEN-mediated genome engineering to generate targeted mice Daniel Sommer & Annika E. Peters & Ann-Kathrin Baumgart & Marc Beyer

# Springer Science+Business Media Dordrecht 2015

Abstract Genetic mouse models are critical for biomedical research to understand gene function and pathophysiology. In the last years, the generation of genetic mouse models has been revolutionized by the emergence of transcription activator-like effector nucleases (TALENs). TALENs are programmable, sequencespecific DNA-binding proteins fused to a non-specific endonuclease domain used as powerful tools for sitespecific induction of DNA double-strand breaks. These result in disruption of the gene product of the targeted locus by mutations induced during repair by error-prone non-homologous end-joining. Alternatively, these DNA double-strand breaks can be exploited to integrate a user-defined sequence by homologous recombination if an appropriate repair plasmid is provided. In this review, we highlight the major technological improvements for genome editing in murine oocytes which have been achieved using TALENs, discuss current Responsible Editor: Natalay Kouprina and Vladimir Larionov Daniel Sommer, Annika E. Peters, and Ann-Kathrin Baumgart contributed equally to this work. D. Sommer : A. E. Peters : A.

TALEN-mediated genome engineering to generate targeted mice.

Genetic mouse models are critical for biomedical research to understand gene function and pathophysiology. In the last years, the generation of geneti...
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