Tohoku

J. exp.

Med.,

1978,

126, 173-175

Systemic

Candidiasis

Produced

Candida

Administration

in

by

Oral

Mice

TAKUSEI UMENAI

Central Clinical Laboratory, Medicine, Sendai 980

UMEANI, in

T.

Mice.

infection

from

was

undetectable

oral

Candida

defence

J.

the

Candidiasis

exp.

Med.,

the

blood,

combined 5 days

even

became

after

oral

under

of

Candida

Oral

liver

in or

condition in

the

antibiotics,

Candida

Administration

173-175 „ŸSystemic

examined

spleen,

detectable

treatment

(2),

was

lungs,

by

126

tract

administration but

Produced 1978,

gastrointestinal in

system,

received and

Systemic

Tohoku

Tohoku University School of

liver

of

severe

and

x-ray

Candida

kidneys

of

albicans

mice

24

damage

kidneys and

hr

after

the

host

when

they

to

of mice

irradiation

administration.„Ÿ

Candida

mice.

dexamethasone

candida;

candida

3

infection

The systemic candidiasis is a serious problem in debilitated patients (Louria et al. 1962; Eras et al. 1972). However, the infection routes or the mechanism of induction of systemic infection are still not clear. The role of Candida coloniza tion in the human gastrointestinal tract (GIT) as a cause of systemic candidiasis should be examined, as Candida is known to be a normal inhabitant of the human GIT and candidemia caused by persorption from the GIT has been reported in both animals and man (Fisher 1930; Deicke and Gemeinhardt 1968; Krause et al. 1969). The present experiment was designed to examine the persorption of Candida albicans (C. albicans) from the GIT in mice and to clarify the mechanism of induction of systemic infection by Candida in the GIT. MATERIALS AND METHODS 6-week-old 40

mice

described the

female

each. in

in to

Ishida

albicans

1. of

C.

was 1977)

phosphate the

mice

Table

colonization

(200 ƒÊg/mouse) and

DDI

The

(1•~101

from

the

approximately to

in to

the

suppress

the

on

Sabouraud's

saline

(pH

7.2)

heart,

lungs,

and

liver,

0.1

ml

orally

(30

of

24 the

day

after

the

spleen

and

kidneys

C. hr

into

at

R)

7 groups the

was

to

enhance

dexamethasone (Umenai

and

suspended

administered Viable

made

at

of

schedules

(U-1-50)

collected

treatments. were

or

albicans

37•Ž,

suspension

to

mg/mouse)

(600

activity. for

divided

according

irradiation

defence

and

one

given

x-ray

agar

g were

treatments

was GIT,

grown

cells/mouse)

20

various

Aminobenzylpenicillin

given

was

weighing subjected

albicans

buffered

mice

mice were

various

orally counts

of times

C. as

scheduled.

RESULTS AND DISCUSSION The results

are shown

blood or organs Received

of mice

in Tables of any

for publication,

1 and 2.

of the

January

7 groups

25, 1978. 173

C. albicans

was not detected

24 hr after

the oral administration.

in the

174

T. Umenai

TABLE1. Incidence of positive cultures of C. albieans in the organs of mice after oral C. albicans administration

* Hours culture. ??

after

inoculation

Number

of

mice

. •õ

Days

examined.

after 9 C.

inoculation. •ö albicans

was

Number detected

of in

the

mice kidneys

with

positive and

liver.

TABLE 2.

Number of viable Candida cells in organs of mice received combined treatments of antibiotics, x-ray irradiation and dexamethasone

* Number

of viable

Candida

cells per organ .

However, it became detectable in the liver and kidneys of mice when they received combination treatments of antibiotics, x-ray irradiation and dexamethasone 3 and 5 days after oral Candida administration. Candida in the GIT is thought to cause systemic infection in two ways; the first is the persorption through the intestinal wall, which usually occurs between 1/2 and 4 hr after oral Candida administration, and the second is the penetrative growth which occurs later (Fisher 1930; Krause et al. 1969). Therefore, the present results seem to suggest that the persorption of C. albicans from the GIT is not likely to occur in normal or even in immunologically suppressed mice, but penetrative growth occurs instead. On the other hand, a larger number of Candida cells are needed for the persorption to occur in mice. Further research on the penetrative growth of C. albicans in the GIT of mice is now in progress. References

1) Eras, P., Goldstein, M.J. & Sherlock, P. (1972) Candida infection of the gastrointestinal tract. Medicine (Baltimore), 51, 367-379. 2) Deicke, P. & Gemeinhardt, H. (1968) Tierexperimentalle Untersuchungen am System des Ductus thoracius zur Frage der enteralen Pilzaufnahme. Mykosen, 2,

Candidiasis

in Mice

175

826. Fisher, V. (1930) Intestinal absorption of viable yeast. Proc. Soc. exp. Biol. Med., 28, 948-951. 4) Krause, W., Matheis, H. & Wulf, K. (1969) Fungaemia and funguria after oral administration of Candida albicans. Lancet, 1, 598-599. 5) Louria, D., Stiff, D. & Bennet, B. (1962) Disseminated candidiasis in the adult. Medicine (Baltimore), 41, 307-337. 6) Umenai, T. & Ishida, N. (1977) Significance of candiduria. Tohoku J. exp. Med., 122, 59-63. 3)

Systemic candidiasis produced by oral Candida administration in mice.

Tohoku J. exp. Med., 1978, 126, 173-175 Systemic Candidiasis Produced Candida Administration in by Oral Mice TAKUSEI UMENAI Central Cli...
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