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Synthesis of multi-functional large pore mesoporous silica nanoparticles as gene carriers
This content has been downloaded from IOPscience. Please scroll down to see the full text. 2014 Nanotechnology 25 055701 (http://iopscience.iop.org/0957-4484/25/5/055701) View the table of contents for this issue, or go to the journal homepage for more
Download details: IP Address: 138.37.211.113 This content was downloaded on 08/06/2014 at 22:04
Please note that terms and conditions apply.
Nanotechnology Nanotechnology 25 (2014) 055701 (12pp)
doi:10.1088/0957-4484/25/5/055701
Synthesis of multi-functional large pore mesoporous silica nanoparticles as gene carriers Sandy B Hartono1 , Meihua Yu1 , Wenyi Gu1 , Jie Yang1 , Ekaterina Strounina2 , Xiaolin Wang3 , Shizhang Qiao4 and Chengzhong Yu1 1
ARC Centre of Excellence for Functional Nanomaterials, Australian Institute for Bioengineering and Nanotechnology, University of Queensland, QLD 4072, Australia 2 Centre for Advanced Imaging, University of Queensland, St Lucia, QLD 4072, Australia 3 Institute for Superconducting and Electronic Materials (ISEM), Australian Institute of Innovative Materials, University of Wollongong, Innovation Campus Squires Way, North Wollongong, NSW 2500, Australia 4 School of Chemical Engineering, University of Adelaide, SA 5005, Australia E-mail: [email protected] and [email protected] Received 30 October 2013, revised 4 December 2013 Accepted for publication 12 December 2013 Published 9 January 2014 Abstract
The development of functional nanocarriers that can enhance the cellular delivery of a variety of nucleic acid agents is important in many biomedical applications such as siRNA therapy. We report the synthesis of large pore mesoporous silica nanoparticles (LPMSN) loaded with iron oxide and covalently modified by polyethyleneimine (denoted PEI-Fe-LPMSN) as carriers for gene delivery. The LPMSN have a particle size of ∼200 nm and a large pore size of 11 nm. The large pore size is essential for the formation of large iron oxide nanoparticles to increase the magnetic properties and the adsorption capacity of siRNA molecules. The magnetic property facilitates the cellular uptake of nanocarriers under an external magnetic field. PEI is covalently grafted on the silica surface to enhance the nanocarriers’ affinity against siRNA molecules and to improve gene silencing performance. The PEI-Fe-LPMSN delivered siRNA–PLK1 effectively into osteosarcoma cancer cells, leading to cell viability inhibition of 80%, higher compared to the 50% reduction when the same dose of siRNA was delivered by a commercial product, oligofectamine. Keywords: nanomaterials, gene delivery, siRNA, polyethyleneimine S Online supplementary data available from stacks.iop.org/Nano/25/055701/mmedia (Some figures may appear in colour only in the online journal)
1. Background
for overcoming the limitation towards the development of efficient siRNA therapy. Various nanovectors such as lipids, polymers and inorganic nanoparticles have been extensively studied [2]. Among these nanovectors, mesoporous silica nanoparticles (MSN) show promising potential as the next-generation gene delivery carriers [3, 4]. Owing to their excellent properties such as high surface area, large and tunable porosity, controllable size and mor-
Small interfering RNA (siRNA) based therapeutics for cancer treatments have gained considerable attention due to the high potency of siRNA in suppressing pathogenic gene expression [1]. Yet, the application of siRNA therapy is limited by the insufficient cellular delivery of genetic molecules [1]. Nanovectors hold great promise 0957-4484/14/055701+12$33.00
1
c 2014 IOP Publishing Ltd Printed in the UK
Nanotechnology 25 (2014) 055701
S B Hartono et al
phology, biological compatibility and biodegradability [5], MSN have opened vast applications in a number of fields such as diagnostics [6], protein supporting materials [7, 8] and drug delivery [9]. So far, MSN with small pore size (
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My IOPscience
Synthesis of multi-functional large pore mesoporous silica nanoparticles as gene carriers
This content has been downloaded from IOPscience. Please scroll down to see the full text. 2014 Nanotechnology 25 055701 (http://iopscience.iop.org/0957-4484/25/5/055701) View the table of contents for this issue, or go to the journal homepage for more
Download details: IP Address: 138.37.211.113 This content was downloaded on 08/06/2014 at 22:04
Please note that terms and conditions apply.
Nanotechnology Nanotechnology 25 (2014) 055701 (12pp)
doi:10.1088/0957-4484/25/5/055701
Synthesis of multi-functional large pore mesoporous silica nanoparticles as gene carriers Sandy B Hartono1 , Meihua Yu1 , Wenyi Gu1 , Jie Yang1 , Ekaterina Strounina2 , Xiaolin Wang3 , Shizhang Qiao4 and Chengzhong Yu1 1
ARC Centre of Excellence for Functional Nanomaterials, Australian Institute for Bioengineering and Nanotechnology, University of Queensland, QLD 4072, Australia 2 Centre for Advanced Imaging, University of Queensland, St Lucia, QLD 4072, Australia 3 Institute for Superconducting and Electronic Materials (ISEM), Australian Institute of Innovative Materials, University of Wollongong, Innovation Campus Squires Way, North Wollongong, NSW 2500, Australia 4 School of Chemical Engineering, University of Adelaide, SA 5005, Australia E-mail: [email protected] and [email protected] Received 30 October 2013, revised 4 December 2013 Accepted for publication 12 December 2013 Published 9 January 2014 Abstract
The development of functional nanocarriers that can enhance the cellular delivery of a variety of nucleic acid agents is important in many biomedical applications such as siRNA therapy. We report the synthesis of large pore mesoporous silica nanoparticles (LPMSN) loaded with iron oxide and covalently modified by polyethyleneimine (denoted PEI-Fe-LPMSN) as carriers for gene delivery. The LPMSN have a particle size of ∼200 nm and a large pore size of 11 nm. The large pore size is essential for the formation of large iron oxide nanoparticles to increase the magnetic properties and the adsorption capacity of siRNA molecules. The magnetic property facilitates the cellular uptake of nanocarriers under an external magnetic field. PEI is covalently grafted on the silica surface to enhance the nanocarriers’ affinity against siRNA molecules and to improve gene silencing performance. The PEI-Fe-LPMSN delivered siRNA–PLK1 effectively into osteosarcoma cancer cells, leading to cell viability inhibition of 80%, higher compared to the 50% reduction when the same dose of siRNA was delivered by a commercial product, oligofectamine. Keywords: nanomaterials, gene delivery, siRNA, polyethyleneimine S Online supplementary data available from stacks.iop.org/Nano/25/055701/mmedia (Some figures may appear in colour only in the online journal)
1. Background
for overcoming the limitation towards the development of efficient siRNA therapy. Various nanovectors such as lipids, polymers and inorganic nanoparticles have been extensively studied [2]. Among these nanovectors, mesoporous silica nanoparticles (MSN) show promising potential as the next-generation gene delivery carriers [3, 4]. Owing to their excellent properties such as high surface area, large and tunable porosity, controllable size and mor-
Small interfering RNA (siRNA) based therapeutics for cancer treatments have gained considerable attention due to the high potency of siRNA in suppressing pathogenic gene expression [1]. Yet, the application of siRNA therapy is limited by the insufficient cellular delivery of genetic molecules [1]. Nanovectors hold great promise 0957-4484/14/055701+12$33.00
1
c 2014 IOP Publishing Ltd Printed in the UK
Nanotechnology 25 (2014) 055701
S B Hartono et al
phology, biological compatibility and biodegradability [5], MSN have opened vast applications in a number of fields such as diagnostics [6], protein supporting materials [7, 8] and drug delivery [9]. So far, MSN with small pore size (
