RHEUMATOLOGY
Rheumatology 2014;53:165172 doi:10.1093/rheumatology/ket334 Advance Access publication 17 October 2013
Original article Synovial joint fluid cytokine levels in hip disease Hirohito Abe1, Takashi Sakai2, Wataru Ando3, Masaki Takao2, Takashi Nishii1, Nobuo Nakamura4, Toshimitsu Hamasaki5, Hideki Yoshikawa2 and Nobuhiko Sugano1 Abstract
Results. All measured cytokine levels in RDC were significantly higher than those in OA (P < 0.05). Terminal OA showed higher cytokine levels than those in early OA (P < 0.05). IL-6 and TNF-a levels in the ON group with >3 mm collapse were higher than those found in the ON group with 70 years old were included, and we compared RDC with terminal OA [RDC: 24 hips, mean (S.D.) 77 (4) years; terminal OA: 18 hips, 75 (4) years; P = 0.09]. RDC hips showed significantly higher levels of IL-1b [RDC: mean (S.D.) 824 (1283) pg/ml, terminal OA: 283 (361) pg/ml, P = 0.007], IL-8 [RDC: 3829 (2675) pg/ml, terminal OA: 1323 (999) pg/ml, P = 0.002] and TNF-a [RDC: 1070 (1579) pg/ml, terminal OA: 393 (372) pg/ml, P = 0.02]. There was no difference for IL-6 level [RDC: 1435 (1636) pg/ml, terminal OA: 1191 (765) pg/ml, P = 0.43].
Discussion Some authors have examined the cytokine levels in knee joint synovial fluid. The levels of IL-1b, IL-8 and TNF-a in the RA knee joint fluid were significantly higher than those in the OA knee [6, 7]. High-grade destruction of the OA knee showed significantly higher levels of IL-1b than did OA knees with low-grade destruction [22]. However, there were few reports concerning cytokine levels in the hip joint. Although hip joint fluid IL-1b levels with acetabular dysplasia have been measured, the samples were collected after injection of 10 ml of saline inside the joint capsule [15]. Therefore they did not have an accurate cytokine level. In the present study, joint fluid was obtained from 82% of the hips with early OA without injection of saline. It has been reported that joint fluid IL-1b levels for four hips with RDC were higher than for hips with OA [16]. In the present study, IL-1b levels were also significantly higher in 33 hips with RDC than in 37 hips with terminal OA. No previous study evaluated other inflammatory cytokine levels, including IL-6, IL-8 and TNF-a, in other hip
167
Downloaded from http://rheumatology.oxfordjournals.org/ at Univ of Iowa-Law Library on June 6, 2015
Comparisons between each hip disease (P values)
RA-ON
Hip synovial joint fluid cytokine levels
Hirohito Abe et al.
TABLE 2 Statistical comparison of cytokine levels in synovial fluid between OA stages Early OA 20
Number of hips IL-1b, median (range), pg/ml IL-6, median (range), pg/ml IL-8, median (range), pg/ml TNF-a, median (range), pg/ml
50 44 163 90
(0413) (01127) (42737) (3500)
Terminal OA 37
P-valuea
86 542 190 207
0.004
Rheumatology 2014;53:165172 doi:10.1093/rheumatology/ket334 Advance Access publication 17 October 2013
Original article Synovial joint fluid cytokine levels in hip disease Hirohito Abe1, Takashi Sakai2, Wataru Ando3, Masaki Takao2, Takashi Nishii1, Nobuo Nakamura4, Toshimitsu Hamasaki5, Hideki Yoshikawa2 and Nobuhiko Sugano1 Abstract
Results. All measured cytokine levels in RDC were significantly higher than those in OA (P < 0.05). Terminal OA showed higher cytokine levels than those in early OA (P < 0.05). IL-6 and TNF-a levels in the ON group with >3 mm collapse were higher than those found in the ON group with 70 years old were included, and we compared RDC with terminal OA [RDC: 24 hips, mean (S.D.) 77 (4) years; terminal OA: 18 hips, 75 (4) years; P = 0.09]. RDC hips showed significantly higher levels of IL-1b [RDC: mean (S.D.) 824 (1283) pg/ml, terminal OA: 283 (361) pg/ml, P = 0.007], IL-8 [RDC: 3829 (2675) pg/ml, terminal OA: 1323 (999) pg/ml, P = 0.002] and TNF-a [RDC: 1070 (1579) pg/ml, terminal OA: 393 (372) pg/ml, P = 0.02]. There was no difference for IL-6 level [RDC: 1435 (1636) pg/ml, terminal OA: 1191 (765) pg/ml, P = 0.43].
Discussion Some authors have examined the cytokine levels in knee joint synovial fluid. The levels of IL-1b, IL-8 and TNF-a in the RA knee joint fluid were significantly higher than those in the OA knee [6, 7]. High-grade destruction of the OA knee showed significantly higher levels of IL-1b than did OA knees with low-grade destruction [22]. However, there were few reports concerning cytokine levels in the hip joint. Although hip joint fluid IL-1b levels with acetabular dysplasia have been measured, the samples were collected after injection of 10 ml of saline inside the joint capsule [15]. Therefore they did not have an accurate cytokine level. In the present study, joint fluid was obtained from 82% of the hips with early OA without injection of saline. It has been reported that joint fluid IL-1b levels for four hips with RDC were higher than for hips with OA [16]. In the present study, IL-1b levels were also significantly higher in 33 hips with RDC than in 37 hips with terminal OA. No previous study evaluated other inflammatory cytokine levels, including IL-6, IL-8 and TNF-a, in other hip
167
Downloaded from http://rheumatology.oxfordjournals.org/ at Univ of Iowa-Law Library on June 6, 2015
Comparisons between each hip disease (P values)
RA-ON
Hip synovial joint fluid cytokine levels
Hirohito Abe et al.
TABLE 2 Statistical comparison of cytokine levels in synovial fluid between OA stages Early OA 20
Number of hips IL-1b, median (range), pg/ml IL-6, median (range), pg/ml IL-8, median (range), pg/ml TNF-a, median (range), pg/ml
50 44 163 90
(0413) (01127) (42737) (3500)
Terminal OA 37
P-valuea
86 542 190 207
0.004
