Appl Microbiol Biotechnol DOI 10.1007/s00253-014-6001-3
MINI-REVIEW
Synergistic proteins for the enhanced enzymatic hydrolysis of cellulose by cellulase In Jung Kim & Hee Jin Lee & In-Geol Choi & Kyoung Heon Kim
Received: 31 May 2014 / Revised: 28 July 2014 / Accepted: 29 July 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract Reducing the enzyme loadings for enzymatic saccharification of lignocellulose is required for economically feasible production of biofuels and biochemicals. One strategy is addition of small amounts of synergistic proteins to cellulase mixtures. Synergistic proteins increase the activity of cellulase without causing significant hydrolysis of cellulose. Synergistic proteins exert their activity by inducing structural modifications in cellulose. Recently, synergistic proteins from various biological sources, including bacteria, fungi, and plants, were identified based on genomic data, and their synergistic activities were investigated. Currently, an up-todate overview of several aspects of synergistic proteins, such as their functions, action mechanisms and synergistic activity, are important for future industrial application. In this review, we summarize the current state of research on four synergistic proteins: carbohydrate-binding modules, plant expansins, expansin-like proteins, and Auxiliary Activity family 9 (formerly GH61) proteins. This review provides critical information to aid in promoting research on the development of efficient and industrially feasible synergistic proteins. Keywords Synergistic protein . Cellulose hydrolysis . Cellulase . Carbohydrate-binding module . Expansin . Auxiliary Activity family 9 . GH61
Introduction Lignocellulose is promising renewable biomass for producing biofuels and biochemicals. The bioconversion of lignocellulose to fuels and chemicals is performed via three main I. J. Kim : H. J. Lee : I.
MINI-REVIEW
Synergistic proteins for the enhanced enzymatic hydrolysis of cellulose by cellulase In Jung Kim & Hee Jin Lee & In-Geol Choi & Kyoung Heon Kim
Received: 31 May 2014 / Revised: 28 July 2014 / Accepted: 29 July 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract Reducing the enzyme loadings for enzymatic saccharification of lignocellulose is required for economically feasible production of biofuels and biochemicals. One strategy is addition of small amounts of synergistic proteins to cellulase mixtures. Synergistic proteins increase the activity of cellulase without causing significant hydrolysis of cellulose. Synergistic proteins exert their activity by inducing structural modifications in cellulose. Recently, synergistic proteins from various biological sources, including bacteria, fungi, and plants, were identified based on genomic data, and their synergistic activities were investigated. Currently, an up-todate overview of several aspects of synergistic proteins, such as their functions, action mechanisms and synergistic activity, are important for future industrial application. In this review, we summarize the current state of research on four synergistic proteins: carbohydrate-binding modules, plant expansins, expansin-like proteins, and Auxiliary Activity family 9 (formerly GH61) proteins. This review provides critical information to aid in promoting research on the development of efficient and industrially feasible synergistic proteins. Keywords Synergistic protein . Cellulose hydrolysis . Cellulase . Carbohydrate-binding module . Expansin . Auxiliary Activity family 9 . GH61
Introduction Lignocellulose is promising renewable biomass for producing biofuels and biochemicals. The bioconversion of lignocellulose to fuels and chemicals is performed via three main I. J. Kim : H. J. Lee : I.
