APPLIED AND ENVIRONMENTAL MICROBIOLOGY, June 1978, p. 1128-1135 0099-2240/78/0035-1128$02.00/0 Copyright © 1978 American Society for Microbiology
Vol. 35, No. 6
Printed in U.S.A.
Survival of Bacillus licheniformis on Human Skin D. J. BIBEL,* D. J. LOVELL, AND R. J. SMILJANIC Department of Dermatology Research, Letterman Army Institute of Research, Presidio of San Francisco, California 94129
Received for publication 24 January 1978
The colonization and survival of Bacitlus species, members of the cutaneous microbial community of humans, were investigated by applying spores of Bacillus licheniformis to the forearms of volunteers. Four strains were tested, including the bacitracin producer ATCC 10716 and its bacitracin-negative mutant. Germination occurred within 24 h. Significant differences in survival population and duration were found among the test strains; however, ATCC 10716 and its mutant produced statistically similar survival curves. In general, an inoculum density of 104 colony-forming units per cm2 allowed survival for at least 2 weeks. Individual variation was extreme, for one subject harbored bacilli for over 2 months and another eliminated the microorganism within 3 days. Individuals could be differentiated into long-term (>21 days) and short-term (
Vol. 35, No. 6
Printed in U.S.A.
Survival of Bacillus licheniformis on Human Skin D. J. BIBEL,* D. J. LOVELL, AND R. J. SMILJANIC Department of Dermatology Research, Letterman Army Institute of Research, Presidio of San Francisco, California 94129
Received for publication 24 January 1978
The colonization and survival of Bacitlus species, members of the cutaneous microbial community of humans, were investigated by applying spores of Bacillus licheniformis to the forearms of volunteers. Four strains were tested, including the bacitracin producer ATCC 10716 and its bacitracin-negative mutant. Germination occurred within 24 h. Significant differences in survival population and duration were found among the test strains; however, ATCC 10716 and its mutant produced statistically similar survival curves. In general, an inoculum density of 104 colony-forming units per cm2 allowed survival for at least 2 weeks. Individual variation was extreme, for one subject harbored bacilli for over 2 months and another eliminated the microorganism within 3 days. Individuals could be differentiated into long-term (>21 days) and short-term (
