Testis, Varicocele and Stones
Structural Study of Gubernaculum Testis in Fetuses with Prune Belly Syndrome Suelen F. Costa, Waldemar S. Costa, Francisco J. B. Sampaio and Luciano A. Favorito* From the Urogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, Brazil
Abbreviations and Acronyms CGRP ¼ calcitonin gene-related peptide PBS ¼ prune belly syndrome Vv ¼ volumetric density WPC ¼ weeks of gestation Accepted for publication June 30, 2014. Study received approval from the institutional ethical committee for human experimentation. Supported by grants from the National Council for Scientific and Technological Development, Brazil, and the Rio de Janeiro State Research Foundation. * Correspondence: Rua Professor Gabizo, 104/201, Tijuca, Rio de Janeiro, Rio de Janeiro, Brazil, CEP: 20271-320 (telephone: 55(21) 22644679; FAX: 55(21) 38728802; e-mail: [email protected]).
Purpose: We compared and contrasted the structure of the gubernaculum testis in fetuses with prune belly syndrome and normal controls. Materials and Methods: We studied a total of 6 gubernacula from 3 male fetuses with prune belly syndrome and a total of 14 from 7 male fetuses without an anomaly. Gubernacular specimens were cut into 5 mm sections and stained with Masson trichrome to quantify connective tissue and smooth muscle cells, with Weigert stain to observe elastic fibers and with picrosirius red with polarization to observe collagen. Immunohistochemical analysis was done with tubulin to observe the nerves. Images were captured with a BX51 microscope and DP70 camera (OlympusÒ). Stereological analysis was done with Image-Pro and ImageJ (MediaCyberneticsÒ) using a grid to determine volumetric density. Means were statistically compared with the Mann-Whitney test. All tests were 2-sided with p
Structural Study of Gubernaculum Testis in Fetuses with Prune Belly Syndrome Suelen F. Costa, Waldemar S. Costa, Francisco J. B. Sampaio and Luciano A. Favorito* From the Urogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, Brazil
Abbreviations and Acronyms CGRP ¼ calcitonin gene-related peptide PBS ¼ prune belly syndrome Vv ¼ volumetric density WPC ¼ weeks of gestation Accepted for publication June 30, 2014. Study received approval from the institutional ethical committee for human experimentation. Supported by grants from the National Council for Scientific and Technological Development, Brazil, and the Rio de Janeiro State Research Foundation. * Correspondence: Rua Professor Gabizo, 104/201, Tijuca, Rio de Janeiro, Rio de Janeiro, Brazil, CEP: 20271-320 (telephone: 55(21) 22644679; FAX: 55(21) 38728802; e-mail: [email protected]).
Purpose: We compared and contrasted the structure of the gubernaculum testis in fetuses with prune belly syndrome and normal controls. Materials and Methods: We studied a total of 6 gubernacula from 3 male fetuses with prune belly syndrome and a total of 14 from 7 male fetuses without an anomaly. Gubernacular specimens were cut into 5 mm sections and stained with Masson trichrome to quantify connective tissue and smooth muscle cells, with Weigert stain to observe elastic fibers and with picrosirius red with polarization to observe collagen. Immunohistochemical analysis was done with tubulin to observe the nerves. Images were captured with a BX51 microscope and DP70 camera (OlympusÒ). Stereological analysis was done with Image-Pro and ImageJ (MediaCyberneticsÒ) using a grid to determine volumetric density. Means were statistically compared with the Mann-Whitney test. All tests were 2-sided with p
