Mol Neurobiol DOI 10.1007/s12035-014-9021-1
Strategy to Suppress Oxidative Damage-Induced Neurotoxicity in PC12 Cells by Curcumin: the Role of ROS-Mediated DNA Damage and the MAPK and AKT Pathways Xiao-yan Fu & Ming-feng Yang & Ming-zhi Cao & Da-wei Li & Xiao-yi Yang & Jing-yi Sun & Zong-yong Zhang & Lei-lei Mao & Shuai Zhang & Feng-ze Wang & Feng Zhang & Cun-dong Fan & Bao-liang Sun
Received: 22 September 2014 / Accepted: 19 November 2014 # Springer Science+Business Media New York 2014
Abstract Oxidative damage plays a key role in causation and progression of neurodegenerative diseases. Inhibition of oxidative stress represents one of the most effective ways in treating human neurologic diseases. Herein, we evaluated the protective effect of curcumin on PC12 cells against H2O2-induced neurotoxicity and investigated its underlying mechanism. The results indicated that curcumin pre-treatment significantly suppressed H2O2-induced cytotoxicity, inhibited the loss of mitochondrial membrane potential (Δψm) through regulation of Bcl-2 family expression, and ultimately reversed
Xiao-yan Fu and Ming-feng Yang are co-first authors.
H2O2-induced apoptotic cell death in PC12 cells. Attenuation of caspase activation, poly(ADP-ribose) polymerase (PARP) cleavage, DNA damage, and accumulation of reactive oxygen species (ROS) all confirmed its protective effects. Moreover, curcumin markedly alleviated the dysregulation of the MAPK and AKT pathways induced by H2O2. Taken together, our findings suggest that the strategy of using curcumin could be a highly effective way in combating oxidative damagemediated human neurodegenerative diseases. Keywords Curcumin . Oxidative damage . Apoptosis . Reactive oxygen species . Neurodegenerative diseases
Electronic supplementary material The online version of this article (doi:10.1007/s12035-014-9021-1) contains supplementary material, which is available to authorized users. X.
Strategy to Suppress Oxidative Damage-Induced Neurotoxicity in PC12 Cells by Curcumin: the Role of ROS-Mediated DNA Damage and the MAPK and AKT Pathways Xiao-yan Fu & Ming-feng Yang & Ming-zhi Cao & Da-wei Li & Xiao-yi Yang & Jing-yi Sun & Zong-yong Zhang & Lei-lei Mao & Shuai Zhang & Feng-ze Wang & Feng Zhang & Cun-dong Fan & Bao-liang Sun
Received: 22 September 2014 / Accepted: 19 November 2014 # Springer Science+Business Media New York 2014
Abstract Oxidative damage plays a key role in causation and progression of neurodegenerative diseases. Inhibition of oxidative stress represents one of the most effective ways in treating human neurologic diseases. Herein, we evaluated the protective effect of curcumin on PC12 cells against H2O2-induced neurotoxicity and investigated its underlying mechanism. The results indicated that curcumin pre-treatment significantly suppressed H2O2-induced cytotoxicity, inhibited the loss of mitochondrial membrane potential (Δψm) through regulation of Bcl-2 family expression, and ultimately reversed
Xiao-yan Fu and Ming-feng Yang are co-first authors.
H2O2-induced apoptotic cell death in PC12 cells. Attenuation of caspase activation, poly(ADP-ribose) polymerase (PARP) cleavage, DNA damage, and accumulation of reactive oxygen species (ROS) all confirmed its protective effects. Moreover, curcumin markedly alleviated the dysregulation of the MAPK and AKT pathways induced by H2O2. Taken together, our findings suggest that the strategy of using curcumin could be a highly effective way in combating oxidative damagemediated human neurodegenerative diseases. Keywords Curcumin . Oxidative damage . Apoptosis . Reactive oxygen species . Neurodegenerative diseases
Electronic supplementary material The online version of this article (doi:10.1007/s12035-014-9021-1) contains supplementary material, which is available to authorized users. X.
