JOURNAL OF VIROLOGY, OCt. 1976, p. 78-85 Copyright C) 1976 American Society for Microbiology
Vol. 20, No. 3
Printed in U.S.A.
Simian Virus 40 DNA Replication in Nuclear Monolayers DONALD J. LE BLANC' * AND MAXINE F. SINGER National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014
Received for publication 19 April 1976
Simian virus 40 DNA replication has been studied in nuclear monolayers prepared by treatment of monolayers of BSC-1 monkey kidney cells with Nonidet P-40. These nuclear monolayers incorporated [3HITTP into two types of viral replicative intermediates that sediment as 25-26S and 22-23S species, respectively, in neutral sucrose gradients. The 22-23S species behaves, in dye buoyant density equilibrium gradients, as a late replicative intermediate. Examination of both species in alkaline sucrose gradients revealed the presence of two types of newly synthesized strands: (i) 4-7S strands and (ii) full-length, or nearly fulllength, 10-16S strands. At low TTP concentrations (