LAB/IN VITRO RESEARCH e-ISSN 1643-3750 © Med Sci Monit, 2015; 21: 1002-1007 DOI: 10.12659/MSM.893754

Silencing of Bmi-1 Gene Enhances Chemotherapy Sensitivity in Human Glioblastoma Cells

Received: 2015.02.02 Accepted: 2015.03.19 Published: 2015.04.06

Authors’ Contribution: Study Design  A Data Collection  B Analysis  C Statistical Data Interpretation  D Manuscript Preparation  E Literature Search  F Funds Collection  G

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Yang Hong Chao Shang Yi-xue Xue Yun-hui Liu

1 Department of Neurosurgery, Shengjing Hospital, China Medical University, Shenyang, Liaoning, P.R. China 2 Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, Liaoning, P.R. China

Yun-hui Liu, e-mail: [email protected] This work was supported by the National Natural Science Foundation of China (81172408, 81301862)

The aim of this study was to determine the influence of the BMI1 gene on chemotherapy sensitivity in human glioma cells. The expression of the BMI1 gene in 41 cases of human brain glioma was determined by quantitative real-time PCR. The silencing effect of RNA interference on the BMI1 gene was detected by Western blot. Methyl thiazolyl tetrazolium assay (MTT) and flow cytometry methods were used to determine the cell viability and apoptosis rate of the U251 cells with BMI1 silencing. After those U251 cells were treated with Cisplatin (DDP), the cell viability and apoptosis rate were further detected. The BMI1 mRNA in glioma was remarkably up-regulated, 176.3% as much as that in peri-cancerous tissues (P

Silencing of Bmi-1 gene enhances chemotherapy sensitivity in human glioblastoma cells.

The aim of this study was to determine the influence of the BMI1 gene on chemotherapy sensitivity in human glioma cells...
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