Chan et al. Nanoscale Research Letters (2017) 12:344 DOI 10.1186/s11671-017-2121-x
NANO EXPRESS
Open Access
SERS Detection of Biomolecules by Highly Sensitive and Reproducible Raman-Enhancing Nanoparticle Array Tzu-Yi Chan1, Ting-Yu Liu1*, Kuan-Syun Wang1, Kun-Tong Tsai2, Zhi-Xin Chen2, Yu-Chi Chang2, Yi-Qun Tseng1, Chih-Hao Wang1, Juen-Kai Wang2,3 and Yuh-Lin Wang2,4
Abstract: This paper describes the preparation of nanoarrays composed of silver nanoparticles (AgNPs: 20–50 nm) for use as surface-enhanced Raman scattering (SERS) substrates. The AgNPs were grown on porous anodic aluminum oxide (AAO) templates by electrochemical plating, and the inter-channel gap of AAO channels is between 10 and 20 nm. The size and interparticle gap of silver particles were adjusted in order to achieve optimal SERS signals and characterized by scanning electron microscopy, atomic force microscopy, and Raman spectroscopy. The fluctuation of SERS intensity is about 10–20% when measuring adenine solutions, showing a great reproducible SERS sensing. The nanoparticle arrays offer a large potential for practical applications as shown by the SERS-based quantitative detection and differentiation of adenine (A), thymine (T), cytosine (C), guanine (G), β-carotene, and malachite green. The respective detection limits are
NANO EXPRESS
Open Access
SERS Detection of Biomolecules by Highly Sensitive and Reproducible Raman-Enhancing Nanoparticle Array Tzu-Yi Chan1, Ting-Yu Liu1*, Kuan-Syun Wang1, Kun-Tong Tsai2, Zhi-Xin Chen2, Yu-Chi Chang2, Yi-Qun Tseng1, Chih-Hao Wang1, Juen-Kai Wang2,3 and Yuh-Lin Wang2,4
Abstract: This paper describes the preparation of nanoarrays composed of silver nanoparticles (AgNPs: 20–50 nm) for use as surface-enhanced Raman scattering (SERS) substrates. The AgNPs were grown on porous anodic aluminum oxide (AAO) templates by electrochemical plating, and the inter-channel gap of AAO channels is between 10 and 20 nm. The size and interparticle gap of silver particles were adjusted in order to achieve optimal SERS signals and characterized by scanning electron microscopy, atomic force microscopy, and Raman spectroscopy. The fluctuation of SERS intensity is about 10–20% when measuring adenine solutions, showing a great reproducible SERS sensing. The nanoparticle arrays offer a large potential for practical applications as shown by the SERS-based quantitative detection and differentiation of adenine (A), thymine (T), cytosine (C), guanine (G), β-carotene, and malachite green. The respective detection limits are
