Serologic Survey for Transmissible Gastroenteritis Virus Neutralizing Antibodies in Selected Feral and Domestic Swine Sera in the Southern United States Author(s): R. D. Woods, E. C. Pirtle, J. M. Sacks, and E. P. J. Gibbs Source: Journal of Wildlife Diseases, 26(3):420-422. Published By: Wildlife Disease Association DOI: http://dx.doi.org/10.7589/0090-3558-26.3.420 URL: http://www.bioone.org/doi/full/10.7589/0090-3558-26.3.420

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Journal

Serologic

Survey

Neutralizing Swine

for Transmissible

Antibodies

Sera

R. D. Woods,

E. C. Pirtle,’

Research

Service,

National

Diseases,

College

of Veterinary

ABSTRACT:

Serum

and

domestic

feral

swine

in

Georgia

plaque reduction (VN) antibodies sible

and

against virus

collected

from were

virus porcine

(TGE).

from

feral assayed

of

antibodies for TGE virus, but expenimentally infected feral swine seroconverted. None of 665 samples from domestic swine contained TGE-VN antibodies. These results indicate feral swine are not a significant reservoir for TGE virus

in

southern infected

against

TGE.

states,

and

Key words: sible gastroentenitis vey, Sus scrofa.

but

are

capable

developing

Reservoir, virus,

VN

of

swine

pp.

420-422

Virus

Rorida

the

USA

from Georgia

blood

was

three

feral and

swine in domestic

taken

from

locations

reon

Texas, swine

262

feral

in Florida,

184

swine on Ossabaw Island, Georgia, 1 14 feral swine from 12 locations

Texas were

be-

32612,

Agricultural of Infectious

of this report is to present the a serologic survey conducted

from

feral and

antibodies

antibodies, feral swine,

Gainesville,

in Florida. Venous

560

contained

VN

coming

1990,

U.S. Department of Agriculture, Iowa 50010, USA; 2 Department

sera collected Florida, and

neutralizing transmis-

swine

1

purpose sults of

and

None

feral

26(3),

States

of Rorida,

in Florida

Diseases,

and Domestic

P.O. Box 70, Ames,

University

Texas

their the

Feral

and E. P. J. Gibbs,2

Center,

collected scrofa)

for

gastroentenitis

samples

Disease

Medicine,

(Sus

by

United

J. M. Sacks,’

Animal

samples

swine

Gastroenteritis

in Selected

in the Southern

of Wildlife

in

(USA). In addition, serum samples taken from 665 domestic swine from same

general

areas

the

feral

swine

were

captured in Florida. These samples were collected from nine different locations and none of the swine were known to be exposed to TGE.

transmisserosur-

Feral swine (Sus scrofa) can be found throughout the southern United States as free-ranging populations. Serologic sur-

After collection, blood clot, centrifuged at 1,500 removed. Sera were heat

veys

C for 30 mm) and diluted from 1:2 through 1:1,024 in serum-free Eagle’s minimum essential medium (MEM) with Earle’s ad-

of feral

swine

sera

from

several

areas

demonstrate they may contain antibodies against various pathogens (Clark et al., 1983; Corn et al., 1986). In many areas, feral

swine

may

mestic swin1e modified-live troenteritis covered

come

in contact

that were porcine

(TGE) from TGE

vaccinated transmissible

virus, pigs or perhaps

tact with clinically affected no references were found lence of virus neutralizing to TGE virus the complete nature

in

1962),

starlings

flies dition, TGE virus

(Cough swine

dogs

it

and

with a gas-

that have reeven in con-

pigs. However, on the preva(VN) antibodies

has

and

been

foxes

(Pilchard,

1965)

and

1983).

experimentally

(Underdahl

shown

to

(Haelterman,

Jorgenson,

virus have been in their respiratory

postexposure

do-

in feral swine sera. While host range of TGE virus in

is unknown,

survive

with

shown tract

house In

ad-

infected

with

to harbor for 104

the days

et al.,

1975).

The 420

ditives land,

(GIBCO New York

samples

were

Laboratories, 14072, USA).

mixed

of Eagle’s MEM serum containing

with

plaque

units

forming

was allowed to g and the serum inactivated (56

with

Grand IsThe diluted

an equal

volume

1% bovine approximately (pfu)

of

fetal the

calf 100 Miller

strain 1680 44691, Following mixtures swine

of TGE virus (Linda Saif, FAHRP, Madison Avenue, Wooster, Ohio USA) and held at 37 C for 60 mm. incubation, the serum-virus were inoculated onto 5-day-old testes cell cultures in 60 x 15 mm

tissue

culture

dishes

and

allowed

to adsorb

for 60 mm at 37 C in a humidified incubator. After incubation, the cell

CO2 mono-

layer gle’s

in Eawere

was overlaid with serum free-MEM.

incubated

at 37

methanol

and

C for stained

0.5% The 2 days, with

agar dishes fixed 0.1%

with crystal

SHORT

violet.

The

viral

plaques

were

counted,

and

the 50% virus neutralization titer was calculated and

(VN) end recorded.

point Virus

neutralizing considered

or

were

titers of positive. 5-mo-old feral

Two

lated

with

3 x

10

1:4

swine

pfu

of

TGE virus via stomach hr later two additional were added as contact from

of feral

levels

of

None

Clinical among

greater were

VN

signs captured

antibodies of

by of

of

the

28 days PE 1:8 (Table

clinical

signs

Although from 560

four

had

TGE

virus.

from 665 doalso seronegClinical signs in any of the

seroconverted

all four pigs had a VN 1). None of the pigs of TGE

during

the

none of the serum feral swine in Florida,

Georgia had VN antibodies virus, there is no evidence

they

capable

of

being

TGE they

ples

collected

from

idea is they were of virulent were clini-

they seroof VN annot only are

domestic

the

they virus

are

to sus-

serum swine

Pig 35

samin Flor-

ida also were seronegative for TGE antibodies. Lack of VN antibodies for TGE in both feral and domestic swine indicates the

Serologic survey for transmissible gastroenteritis virus neutralizing antibodies in selected feral and domestic swine sera in the southern United States.

Serum samples collected from feral and domestic swine (Sus scrofa) in Florida and feral swine in Georgia and Texas were assayed by plaque reduction fo...
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