Serologic Survey for Transmissible Gastroenteritis Virus Neutralizing Antibodies in Selected Feral and Domestic Swine Sera in the Southern United States Author(s): R. D. Woods, E. C. Pirtle, J. M. Sacks, and E. P. J. Gibbs Source: Journal of Wildlife Diseases, 26(3):420-422. Published By: Wildlife Disease Association DOI: http://dx.doi.org/10.7589/0090-3558-26.3.420 URL: http://www.bioone.org/doi/full/10.7589/0090-3558-26.3.420
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Journal
Serologic
Survey
Neutralizing Swine
for Transmissible
Antibodies
Sera
R. D. Woods,
E. C. Pirtle,’
Research
Service,
National
Diseases,
College
of Veterinary
ABSTRACT:
Serum
and
domestic
feral
swine
in
Georgia
plaque reduction (VN) antibodies sible
and
against virus
collected
from were
virus porcine
(TGE).
from
feral assayed
of
antibodies for TGE virus, but expenimentally infected feral swine seroconverted. None of 665 samples from domestic swine contained TGE-VN antibodies. These results indicate feral swine are not a significant reservoir for TGE virus
in
southern infected
against
TGE.
states,
and
Key words: sible gastroentenitis vey, Sus scrofa.
but
are
capable
developing
Reservoir, virus,
VN
of
swine
pp.
420-422
Virus
Rorida
the
USA
from Georgia
blood
was
three
feral and
swine in domestic
taken
from
locations
reon
Texas, swine
262
feral
in Florida,
184
swine on Ossabaw Island, Georgia, 1 14 feral swine from 12 locations
Texas were
be-
32612,
Agricultural of Infectious
of this report is to present the a serologic survey conducted
from
feral and
antibodies
antibodies, feral swine,
Gainesville,
in Florida. Venous
560
contained
VN
coming
1990,
U.S. Department of Agriculture, Iowa 50010, USA; 2 Department
sera collected Florida, and
neutralizing transmis-
swine
1
purpose sults of
and
None
feral
26(3),
States
of Rorida,
in Florida
Diseases,
and Domestic
P.O. Box 70, Ames,
University
Texas
their the
Feral
and E. P. J. Gibbs,2
Center,
collected scrofa)
for
gastroentenitis
samples
Disease
Medicine,
(Sus
by
United
J. M. Sacks,’
Animal
samples
swine
Gastroenteritis
in Selected
in the Southern
of Wildlife
in
(USA). In addition, serum samples taken from 665 domestic swine from same
general
areas
the
feral
swine
were
captured in Florida. These samples were collected from nine different locations and none of the swine were known to be exposed to TGE.
transmisserosur-
Feral swine (Sus scrofa) can be found throughout the southern United States as free-ranging populations. Serologic sur-
After collection, blood clot, centrifuged at 1,500 removed. Sera were heat
veys
C for 30 mm) and diluted from 1:2 through 1:1,024 in serum-free Eagle’s minimum essential medium (MEM) with Earle’s ad-
of feral
swine
sera
from
several
areas
demonstrate they may contain antibodies against various pathogens (Clark et al., 1983; Corn et al., 1986). In many areas, feral
swine
may
mestic swin1e modified-live troenteritis covered
come
in contact
that were porcine
(TGE) from TGE
vaccinated transmissible
virus, pigs or perhaps
tact with clinically affected no references were found lence of virus neutralizing to TGE virus the complete nature
in
1962),
starlings
flies dition, TGE virus
(Cough swine
dogs
it
and
with a gas-
that have reeven in con-
pigs. However, on the preva(VN) antibodies
has
and
been
foxes
(Pilchard,
1965)
and
1983).
experimentally
(Underdahl
shown
to
(Haelterman,
Jorgenson,
virus have been in their respiratory
postexposure
do-
in feral swine sera. While host range of TGE virus in
is unknown,
survive
with
shown tract
house In
ad-
infected
with
to harbor for 104
the days
et al.,
1975).
The 420
ditives land,
(GIBCO New York
samples
were
Laboratories, 14072, USA).
mixed
of Eagle’s MEM serum containing
with
plaque
units
forming
was allowed to g and the serum inactivated (56
with
Grand IsThe diluted
an equal
volume
1% bovine approximately (pfu)
of
fetal the
calf 100 Miller
strain 1680 44691, Following mixtures swine
of TGE virus (Linda Saif, FAHRP, Madison Avenue, Wooster, Ohio USA) and held at 37 C for 60 mm. incubation, the serum-virus were inoculated onto 5-day-old testes cell cultures in 60 x 15 mm
tissue
culture
dishes
and
allowed
to adsorb
for 60 mm at 37 C in a humidified incubator. After incubation, the cell
CO2 mono-
layer gle’s
in Eawere
was overlaid with serum free-MEM.
incubated
at 37
methanol
and
C for stained
0.5% The 2 days, with
agar dishes fixed 0.1%
with crystal
SHORT
violet.
The
viral
plaques
were
counted,
and
the 50% virus neutralization titer was calculated and
(VN) end recorded.
point Virus
neutralizing considered
or
were
titers of positive. 5-mo-old feral
Two
lated
with
3 x
10
1:4
swine
pfu
of
TGE virus via stomach hr later two additional were added as contact from
of feral
levels
of
None
Clinical among
greater were
VN
signs captured
antibodies of
by of
of
the
28 days PE 1:8 (Table
clinical
signs
Although from 560
four
had
TGE
virus.
from 665 doalso seronegClinical signs in any of the
seroconverted
all four pigs had a VN 1). None of the pigs of TGE
during
the
none of the serum feral swine in Florida,
Georgia had VN antibodies virus, there is no evidence
they
capable
of
being
TGE they
ples
collected
from
idea is they were of virulent were clini-
they seroof VN annot only are
domestic
the
they virus
are
to sus-
serum swine
Pig 35
samin Flor-
ida also were seronegative for TGE antibodies. Lack of VN antibodies for TGE in both feral and domestic swine indicates the