1637
Nucleic Acids Research, Vol. 18, No. 6
of the Chinese hamster small heat shock protein HSP27
Sequence
Josee Lavoie, Pierre Chretien and Jacques Landry* Centre de recherche en cancerologie de l'Universite Laval, I'Hotel-Dieu de Quebec, Quebec GlR 2J6, Canada Submitted February 9, 1990
EMBL accession
Heat shock proteins are believed to play homeostatic functions that help cells sustaining stressful conditions. Gene transfection studies have revealed that consitutive expression of human HSP27 in rodent cells confers a thermoresistant phenotype (1). Genomic and cDNA sequences have been reported for the HSP27 gene from human and rat respectively (2, 3). We report here the sequence of the insert of pH8, a cDNA plasmid contained in a library derived from polyA RNA extracted from Chinese hamster V79 cells after heat shock (4). pH8 was shown to hybridize at high stringency with the human HSP27 cDNA pHS208 (2, 4). The nucleotide sequence consists of 787 nucleotides containing an open reading frame encoding a 213 amino acid protein. The deduced amino acid sequence is 79% and 94% identical to human (2) and rat HSP27 (3) protein respectively.
GGGGGCCCGC GGGACTGGTA TTCAGCGCCG CGCGCCCCTG CCGATCGCTG GAGATCACCG TCCCGGTGTG CAGCCACACA TCGGAACAGT TCTTTATGCT
HAMSTER RAT HUMAN HAMSTER RAT HUMAN
ACAGCCAAGA CCCGGCCCAC CTGGTTGGCC GCCGCGCCCG GCGCGTGTCC GCAAGCACGA GACCCCACCC ATCCGCGGAG CTGGAGCCAA CTTTTGATAC
This work was supported by the Medical Research Council of Canada.
REFERENCES 1. Landry,J., Chr6tien,P., Lambert,H., Hickey,E. and Weber,L.A. (1989) J. Cell Biol. 109, 7-15. 2. Hickey,E., Brandon,S.E., Potter,R., Stein,G., Stein,J. and Weber,L.A. (1986) Nucl. Acids Res. 14, 4127-4145. 3. Gaestel,M., Gross,B., Benndorf,R., Strauss,M., Schunk,W.H., Kraft,R., Otto,A., Bohm,H., Stahl,J., Drabsch,H. and Bielka,H. (1989) Eur. J. Biochem. 179, 209-213. 4. Fornace,A.J.,Jr. and Mitchell,J.B. (1986) Nucd. Acids Res. 14, 5793 -5811.
CCCCAGCTGG CGGATGAGTG CCCGCGGCGG CTCGGAGATC AGACCAAGGA ACCCGGAAAT GGAGGCTCCG GGGGCCAGGA CAGCCATCAC TCGCCACCCC
TGCTGCGGAG CCCCGCTTGC CGCCGAGGGA GCAGCGGAGT CTCACGGTCA CCGGTGCTTT CACTTACCGT GCCCAAATTG
GAACCATTCC GTCGCAGTGG TGGCCCTGGC CGGCAGACGG AGGCGTGGTG ACACGCTGCC CTGCCCAAAA AGCTGGGAAG TCTCTATCAG
TGGAAGCTAC TGCCCCC
PROTEIN SEOUENCE 1 MTERRVPFSLLRSPSWEPFRDWYPAHSRLFDQAFGVPRLPDEWSQWFSAAGWPGYVRPLP-AATAEGPAAVALAA 1 MTERRVPFSLLRSPSWEPFRDWYPAHSRLFDQAFGVPRLPDEWSQWFSAAGWPGYVRPLP-AATAEGPAAVT--1 MTERRVPFSLLRGPSWDPFRDWYP-HSRLFDQAFGLPRLPEEWSQWLGGSSWPGYVRPLPPAAIESPA-----75 71 69
PLAAPAFHRALNRQLSSGVSEIRQTADRWRVSLDVNHFAPEELTVKTKEGVVEITGKHEERQDEHGYISRCFTRK -LAAPAFSRALNRQLSSGVSEIRQTAD-WRVSLDVNHVIPEELTVKTKEGVVEITGKHEERQDEHGYISRCFTRK
-VAAPAYSRALSRQLSSGVSEIRHTADRWRVSLDVNHFAPDELTVKTKDGVVEITGKHEERQDEHGYISRCFTRK
HAMSTER 150 YTLPPGVDPTLVSSSLSPEGTLTVEAPLPKTATQSAEITIPVTFEARAQIGGQEAGKSEQSGAK 213 145 YTLPPGVDPTLVSSSLSPEGTLTVEAPLPKAVTQSTEITIPVTFEARAQIGGPEAGKSEQSGAK 208 RAT 196 142 YTLPPGVDPTQVSSSLSPEGTLTVEAPMPKLATQSNEITIPVTFESRAQ_GGRSCKIR HUMAN
*
To whom correspondence should be addressed
X51747
ACKNOWLEDGEMENT
DNA SEQUENCE GCGCCGCGTG CCCTTCTCGC TCGACCAAGC CTTCGGGGTG CGCCCACTGC CCGCCGCGAC TGCGCTCAAC CGGCAGCTGA ACCACTTCGC TCCGGAGGAG GACGAACATG GCTACATCTC TTCCCTGTCC CCTGAGGGCA CGGTCACTTT CGAGGCCCGC GTAGAAGCCA TCAGCCTGCT GCATCCCCAA ATGTACTTTC TGTTTTTCTC AAATAAAAGT
ACATGACCGA AGCCGCCTCT CGGCTACGTG CCTTCCACCG CTGGACGTCA AGAAAGGCAG TGGTGTCCTC ATCACCATTC
no.
Nucleic Acids Research, Vol. 18, No. 6
of the Chinese hamster small heat shock protein HSP27
Sequence
Josee Lavoie, Pierre Chretien and Jacques Landry* Centre de recherche en cancerologie de l'Universite Laval, I'Hotel-Dieu de Quebec, Quebec GlR 2J6, Canada Submitted February 9, 1990
EMBL accession
Heat shock proteins are believed to play homeostatic functions that help cells sustaining stressful conditions. Gene transfection studies have revealed that consitutive expression of human HSP27 in rodent cells confers a thermoresistant phenotype (1). Genomic and cDNA sequences have been reported for the HSP27 gene from human and rat respectively (2, 3). We report here the sequence of the insert of pH8, a cDNA plasmid contained in a library derived from polyA RNA extracted from Chinese hamster V79 cells after heat shock (4). pH8 was shown to hybridize at high stringency with the human HSP27 cDNA pHS208 (2, 4). The nucleotide sequence consists of 787 nucleotides containing an open reading frame encoding a 213 amino acid protein. The deduced amino acid sequence is 79% and 94% identical to human (2) and rat HSP27 (3) protein respectively.
GGGGGCCCGC GGGACTGGTA TTCAGCGCCG CGCGCCCCTG CCGATCGCTG GAGATCACCG TCCCGGTGTG CAGCCACACA TCGGAACAGT TCTTTATGCT
HAMSTER RAT HUMAN HAMSTER RAT HUMAN
ACAGCCAAGA CCCGGCCCAC CTGGTTGGCC GCCGCGCCCG GCGCGTGTCC GCAAGCACGA GACCCCACCC ATCCGCGGAG CTGGAGCCAA CTTTTGATAC
This work was supported by the Medical Research Council of Canada.
REFERENCES 1. Landry,J., Chr6tien,P., Lambert,H., Hickey,E. and Weber,L.A. (1989) J. Cell Biol. 109, 7-15. 2. Hickey,E., Brandon,S.E., Potter,R., Stein,G., Stein,J. and Weber,L.A. (1986) Nucl. Acids Res. 14, 4127-4145. 3. Gaestel,M., Gross,B., Benndorf,R., Strauss,M., Schunk,W.H., Kraft,R., Otto,A., Bohm,H., Stahl,J., Drabsch,H. and Bielka,H. (1989) Eur. J. Biochem. 179, 209-213. 4. Fornace,A.J.,Jr. and Mitchell,J.B. (1986) Nucd. Acids Res. 14, 5793 -5811.
CCCCAGCTGG CGGATGAGTG CCCGCGGCGG CTCGGAGATC AGACCAAGGA ACCCGGAAAT GGAGGCTCCG GGGGCCAGGA CAGCCATCAC TCGCCACCCC
TGCTGCGGAG CCCCGCTTGC CGCCGAGGGA GCAGCGGAGT CTCACGGTCA CCGGTGCTTT CACTTACCGT GCCCAAATTG
GAACCATTCC GTCGCAGTGG TGGCCCTGGC CGGCAGACGG AGGCGTGGTG ACACGCTGCC CTGCCCAAAA AGCTGGGAAG TCTCTATCAG
TGGAAGCTAC TGCCCCC
PROTEIN SEOUENCE 1 MTERRVPFSLLRSPSWEPFRDWYPAHSRLFDQAFGVPRLPDEWSQWFSAAGWPGYVRPLP-AATAEGPAAVALAA 1 MTERRVPFSLLRSPSWEPFRDWYPAHSRLFDQAFGVPRLPDEWSQWFSAAGWPGYVRPLP-AATAEGPAAVT--1 MTERRVPFSLLRGPSWDPFRDWYP-HSRLFDQAFGLPRLPEEWSQWLGGSSWPGYVRPLPPAAIESPA-----75 71 69
PLAAPAFHRALNRQLSSGVSEIRQTADRWRVSLDVNHFAPEELTVKTKEGVVEITGKHEERQDEHGYISRCFTRK -LAAPAFSRALNRQLSSGVSEIRQTAD-WRVSLDVNHVIPEELTVKTKEGVVEITGKHEERQDEHGYISRCFTRK
-VAAPAYSRALSRQLSSGVSEIRHTADRWRVSLDVNHFAPDELTVKTKDGVVEITGKHEERQDEHGYISRCFTRK
HAMSTER 150 YTLPPGVDPTLVSSSLSPEGTLTVEAPLPKTATQSAEITIPVTFEARAQIGGQEAGKSEQSGAK 213 145 YTLPPGVDPTLVSSSLSPEGTLTVEAPLPKAVTQSTEITIPVTFEARAQIGGPEAGKSEQSGAK 208 RAT 196 142 YTLPPGVDPTQVSSSLSPEGTLTVEAPMPKLATQSNEITIPVTFESRAQ_GGRSCKIR HUMAN
*
To whom correspondence should be addressed
X51747
ACKNOWLEDGEMENT
DNA SEQUENCE GCGCCGCGTG CCCTTCTCGC TCGACCAAGC CTTCGGGGTG CGCCCACTGC CCGCCGCGAC TGCGCTCAAC CGGCAGCTGA ACCACTTCGC TCCGGAGGAG GACGAACATG GCTACATCTC TTCCCTGTCC CCTGAGGGCA CGGTCACTTT CGAGGCCCGC GTAGAAGCCA TCAGCCTGCT GCATCCCCAA ATGTACTTTC TGTTTTTCTC AAATAAAAGT
ACATGACCGA AGCCGCCTCT CGGCTACGTG CCTTCCACCG CTGGACGTCA AGAAAGGCAG TGGTGTCCTC ATCACCATTC
no.
