Vol. 31, No. 3
JOURNAL OF VIROLOGY, Sept. 1979, p. 596-607 0022-538X/79/09-0596/12$02.00/0
Roles of the Simian Virus 40 Tumor Antigens in Transformation of Chinese Hamster Lung Cells: Studies with Simian Virus 40 Doluble Mutants ROBERT G. MARTIN,* VALERIE PETIT SETLOW, AND CAROL A. F. EDWARDS Laboratory of Molecular Biology, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health, Bethesda, Maryland 20205 Received for publication 12 March 1979
Simian virus 40 mutants containing both a tsA mutation (rendering the 90,000 molecular weight [90K] T-antigen thermolabile) and a deletion between 0.54 and 0.59 map units (reducing the size and the amount of the 20K t-antigen) were used to transform Chinese hamster lung cells. The frequencies of transformation by the double mutants were comparable to that of the tsA mutant alone by both the focus and agar assays except when the cells were serum depleted before infection. Growth-arrested cells were transformed (using the agar assay) by the deletion mutants at
JOURNAL OF VIROLOGY, Sept. 1979, p. 596-607 0022-538X/79/09-0596/12$02.00/0
Roles of the Simian Virus 40 Tumor Antigens in Transformation of Chinese Hamster Lung Cells: Studies with Simian Virus 40 Doluble Mutants ROBERT G. MARTIN,* VALERIE PETIT SETLOW, AND CAROL A. F. EDWARDS Laboratory of Molecular Biology, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health, Bethesda, Maryland 20205 Received for publication 12 March 1979
Simian virus 40 mutants containing both a tsA mutation (rendering the 90,000 molecular weight [90K] T-antigen thermolabile) and a deletion between 0.54 and 0.59 map units (reducing the size and the amount of the 20K t-antigen) were used to transform Chinese hamster lung cells. The frequencies of transformation by the double mutants were comparable to that of the tsA mutant alone by both the focus and agar assays except when the cells were serum depleted before infection. Growth-arrested cells were transformed (using the agar assay) by the deletion mutants at
