Int J Clin Exp Pathol 2015;8(9):10929-10934 www.ijcep.com /ISSN:1936-2625/IJCEP0013318
Original Article Role of transcriptional factor Nrf2 in the acute lung injury of mice Minghao Kong*, Jing Mao*, Bijun Luo, Zonghao Qin Department of Emergency, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 20072, China. *Equal contributors. Received July 23, 2015; Accepted August 26, 2015; Epub September 1, 2015; Published September 15, 2015 Abstract: Objective: This study aimed to investigate the expression and role of Nrf2 in the acute lung injury (ALI) of mice. Methods: A total of 60 BABL/c mice were randomly divided into 2 groups: ALI group and control group. In ALI group, ALI was introduced by injection of LPS. Immunohistochemistry was performed to detect Nrf2 expression in the lung; Western blot assay was employed to detect the expression of Nrf2 in the lung homogenate; ELISA was conducted to detect the expression of Nrf2 in the lung homogenate and BALF. Results: As compared to control group, ALI mice had a high Nrf2 expression in the lung as shown in immunohistochemistry, and the Nrf2 expression in the lung homogenate and BALF also increased markedly (P
Original Article Role of transcriptional factor Nrf2 in the acute lung injury of mice Minghao Kong*, Jing Mao*, Bijun Luo, Zonghao Qin Department of Emergency, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 20072, China. *Equal contributors. Received July 23, 2015; Accepted August 26, 2015; Epub September 1, 2015; Published September 15, 2015 Abstract: Objective: This study aimed to investigate the expression and role of Nrf2 in the acute lung injury (ALI) of mice. Methods: A total of 60 BABL/c mice were randomly divided into 2 groups: ALI group and control group. In ALI group, ALI was introduced by injection of LPS. Immunohistochemistry was performed to detect Nrf2 expression in the lung; Western blot assay was employed to detect the expression of Nrf2 in the lung homogenate; ELISA was conducted to detect the expression of Nrf2 in the lung homogenate and BALF. Results: As compared to control group, ALI mice had a high Nrf2 expression in the lung as shown in immunohistochemistry, and the Nrf2 expression in the lung homogenate and BALF also increased markedly (P
