Role of SHIP1 in Invariant NKT Cell Development and Functions.

HHS Public Access Author manuscript Author Manuscript

J Immunol. Author manuscript; available in PMC 2016 September 01. Published in final edited form as: J Immunol. 2015 September 1; 195(5): 2149–2156. doi:10.4049/jimmunol.1500567.

Role of SHIP1 in iNKT cell development and functions1 Courtney K. Anderson#*, Alexander I. Salter#*, Leon E. Toussaint*, Emma C. Reilly*, Céline Fugère*, Neetu Srivastava†, William G. Kerr†,‡, and Laurent Brossay*,3 *Department

of Molecular Microbiology & Immunology, Division of Biology and Medicine, Brown University, Providence, RI 02912

†Departments

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of Pediatrics and Microbiology & Immunology, SUNY Upstate Medical University, Syracuse, NY 13210 ‡Chemistry

#

Department, Syracuse University, Syracuse, NY 13210

These authors contributed equally to this work.

Abstract

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SH2-containing inositol phosphatase-1 (SHIP1) is a 5' inositol phosphatase known to negatively regulate the signaling product of the phosphoinositide-3 kinase (PI3K) pathway, phosphatidylinositol (3,4,5)-trisphosphate (PIP3). SHIP1 is recruited to a large number of inhibitory receptors expressed on invariant natural killer (iNKT) cells. We hypothesized that SHIP1 deletion would have major effects on iNKT cell development by altering the thresholds for positive and negative selection. Germline SHIP1 deletion has been shown to affect T cells, as well as other immune cell populations. However, the role of SHIP1 on T cell function has been controversial and its participation on iNKT cell development and function has not been examined. We evaluated the consequences of SHIP1 deletion on iNKT cells using germline-deficient mice, chimeric mice, and conditionally deficient mice. We found that T cell and iNKT cell development are impaired in germline-deficient animals. However, this phenotype can be rescued by extrinsic expression of SHIP1. In contrast, SHIP1 is required cell autonomously for optimal iNKT cell cytokine secretion. This suggests that SHIP1 calibrates the threshold of iNKT cell reactivity. These data further our understanding of how iNKT cell activation is regulated and provide insights into the biology of this unique cell lineage.

Introduction Author Manuscript

Natural Killer T cells (NKT) are a heterogeneous subset of innate lymphocytes that express NK cell markers, in addition to a TCR. There are multiple functionally distinct categories of

1This work was supported by an NIH research grant (AI46709) to LB and NIH research grants (RO1 HL072523, R01 HL085580, R01 HL107127) and the Paige Arnold Butterfly Run to WGK. 3

Correspondence should be addressed to: Dr. Laurent Brossay, Department of Molecular Microbiology & Immunology, Division of Biology & Medicine, Box G-B618, Brown University, Providence, RI 02912., Phone: (401) 863-9645, Fax: (401) 863-1971, [email protected]. Authorship C.K.A conceived, performed, analyzed the experiments and wrote the paper. A.I.S. conceived, performed, analyzed the experiments and wrote the paper. L.E.T. conceived, performed, and analyzed the experiments. E.C.R. conceived, performed, and analyzed the experiments. C.F. conceived, performed, and analyzed the experiments. N.S performed the experiments. W.G.K provided reagents. L.B. conceived, analyzed the experiments, and wrote the paper. The authors have no conflict of interest to declare.

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NKT cells, including invariant NKT (iNKT) cells, also known as type I NKT cells (1, 2). iNKT cells represent a small fraction of mature T cells within the thymus, spleen, and lymph nodes. However, iNKT cells also accumulate in non-lymphoid organs, including the blood, liver, and gut. In mice, iNKT cells make up a robust population within the liver, ranging between 25–40% of the lymphocytes (3). iNKT cell development occurs in the thymus from the same precursors as conventional T cells, but diverges during positive selection (1, 2, 4). While conventional T cells are selected and restricted by classical MHC peptide antigens presented by thymic cortical epithelial cells, iNKT cells are selected by CD4+CD8+ double positive (DP) cortical thymocytes that express CD1d (1, 2). CD1d is a non-classical MHC class I-like molecule that preferentially binds glycolipid antigens (1, 2). iNKT cells are able to recognize presented glycolipid antigens due to their unique semi-invariant TCR, which consists of an invariant Vα14-Jα18 chain that preferentially dimerizes with a limited number of β-chains, mainly Vβ8.2, Vβ7, and Vβ2 (1, 2, 4).

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In addition to their unique TCR repertoire, iNKT cells are characterized by their ability to rapidly secrete a wide array of cytokines upon stimulation, either through direct TCR activation or indirectly through cytokine signaling. This can include the production of large amounts of IFN-γ and IL-4 (1, 5), allowing iNKT cells to participate in either TH1- or TH2polarized responses. Due to their rapid and diverse responses, iNKT cells are multifunctional and capable of augmenting the participation of other immune cells, including B cells, NK cells, macrophages, and other T cells (6–10).

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The PI3K signaling pathway participates in a number of cellular processes, not limited to cellular activation, development, migration, proliferation, and survival (11, 12). PI3Ks phosphorylate PI(4,5)P2 to PI(3,4,5)P3. PI(3,4,5)P3 is a second messenger that attracts effector proteins containing a Pleckstrin-homology domain and assists in their attachment to the inside of the plasma membrane, leading to downstream cellular responses (11, 13). Together with PTEN (phosphatase and tensin homologue deleted on chromosome 10), SHIP1 is an important negative regulator of PI3K signaling. SHIP1 is expressed predominantly in hematopoietic cells, as well as mesenchymal stem cells and stromal cells (14, 15), and acts by dephosphorylating PI(3,4,5)P3 into PI(3,4)P2 (16). The Src homology 2 (SH2) domain of SHIP1 allows it to associate with both ITAM- and ITIM-containing receptor tails, including SLAM family receptors and TCR associated CD3 chains (17–19). Recently, our lab has shown that SHIP1 is recruited to the ITIM of KLRG1 receptors to negatively regulate intracellular signaling (20).

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Global loss of SHIP1 results in a pleiotropic phenotype, due to its role in the development and function of a number of immune cells. Germline-deficient SHIP1 animals have increased myeloid cell number, attributed to heightened proliferation and survival, but are conversely lymphopenic (21). B cell development and survival are also affected by SHIP1 regulation and BCR signaling is hypersensitive (22, 23). However, the role of SHIP1 in T cell development and functions is less clear. Some studies claim that deletion of SHIP1 affects T cell development, while others report no major developmental issues (21, 24, 25). Using germline-deficient mice, chimeric mice, and conditionally-deficient mice, we revisited these studies with a special emphasis on iNKT cells, which are known to express inhibitory receptors capable of associating with SHIP1. We found that iNKT cell and T cell

J Immunol. Author manuscript; available in PMC 2016 September 01.

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development are significantly impaired in mice with germline deletion of SHIP1. However, this phenotype could be rescued by extrinsic expression of SHIP1. In contrast, SHIP1 was required cell autonomously for optimal iNKT cell cytokine secretion, suggesting that SHIP1 calibrates the threshold of iNKT cell reactivity.

Materials and Methods Mice

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Inbred C57BL/6 mice and BALB/c mice were purchased from Taconic Farms, Inc. (Germantown, NY). SHIP1 mice were described previously and bred to acquire SHIP1+/+, SHIP1+/−, and SHIP1−/− animals (26). C57BL/6 Jα18 heterozygous mice were kindly provided by Dr. M Taniguchi (RIKEN Research Center for Allergy and Immunology, Yokohama, Japan) and bred to obtain Jα18−/− mice in our facility. B6 Thy1.1 mice were acquired from Jackson Laboratories (Bar Harbor, Maine). CD4CreSHIPfl/fl (referred to as CD4cre) and SHIPfl/fl (referred to as Control) samples were described previously (27). All mice were maintained at Brown University in pathogen free facilities. Isolation of Murine Lymphocytes

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Mice were sacrificed by isofluorane treatment and a cardiac puncture was performed before harvesting organs. Thymic lymphocytes were obtained by dissociating thymi with the plunger of a 3mL syringe, passing the sample through mesh, and washing one time in 1% PBS-serum. Spleens were minced with the plunger of a 3mL syringe and samples were passed through mesh. Red blood cells were removed to enrich for splenic lymphocytes using a Lympholyte-M gradient (Cedarlane Laboratories Ltd., Canada) or ammonium chloride treatment. Livers were perfused with 1% PBS-serum prior to harvesting and hepatic lymphocytes were obtained by homogenizing with a gentleMACS Dissociator (Miltenyi Biotec). Liver samples were then washed three times in 1% PBS-serum and layered onto a two-step discontinuous Percoll gradient (Pharmacia Fine Chemicals, Piscataway, NJ). The fat layer was removed and lymphocytes were harvested from the gradient interface and washed one time in 1% PBS-serum. Bone marrow lymphocytes were obtained from the femur and tibia of mice by flushing the interior of the bone with 1% PBS-Serum. Red blood cells were lysed using ammonium chloride to enrich for lymphocytes. To obtain blood lymphocytes, samples from cardiac puncture were treated with ammonium chloride. Lymph nodes were dissociated with the plunger of a 3 mL syringe, passed through mess, and washed once. Cell counts were performed using either a hemocytometer and Trypan Blue (GIBCO) or on an MACSQuant (Miltenyi Biotec) with Propidium Iodide (Miltenyi Biotec) to exclude dead cells.

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Antibodies, Reagents, and Flow Cytometric Analysis Samples were stained with a 2.4G2 blocking antibody and monoclonal antibodies in 1% PBS-serum for 20 minutes on ice in the dark. For samples stained with CD1 tetramer, cells were incubated for 15 minutes at room temperature in the dark, followed by 15 minutes on ice in the dark. Samples requiring intracellular staining were then fixed and permeabilized using CytoFix/CytoPerm (BD Biosciences) and stained with intracellular antibodies for 15 minutes on ice in the dark. Events were collected on either a FACSAria (BD Biosciences) or


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a MACSQuant (Miltenyi Biotec). Data were analyzed using FlowJo (Tree Star Inc.). IL-17A-AlexaFluor488, CD45.2-FITC, HSA-FITC, TCRβ-FITC, TCRVβ2-FITC, CD45.1PE, PLZF-PE, TCRβ-PE, Foxp3-PE-Cy5.5, NK1.1-PerCPCy5.5, TCRβ-PerCPCy5.5, RORγt-PerCPeFluor710, TCRβ8.1/2-PerCPeFluor710, Ly49G2-PerCPeFluor710, IFN-γPE-Cy7, NK1.1-PE-Cy7, T-Bet-PE-Cy7, CD45.1-APC, CD25-APC, IL-4-APC, CD4APCeFluor780, CD44-APCeFluor780, CD45.2-APCeFluor780, CD90.1-APCeFluor780, CD8α-eFluor450, CD3-eFluor450, Ki67-eFluor450, and TNF-α-eFluor540 were purchased from eBioscience (San Diego, CA). Ly49C/I-FITC, Ly49A/D-PE, and CD4-PerCP were purchased from BD Pharmingen. TCRVβ7-PE was purchased from Biolegend. CD1d tetramer loaded with α-GalCer, CD1d tetramer loaded with PBS-57 as well as unloaded controls were provided by the NIH Tetramer Facility. In vivo Proliferation Analysis

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Recipient mice were sub-lethally irradiated with 7.5 Gy and placed on oral Sulfamethoxazole and Trimethoprim (Hi-tech Pharmacal) treatment. On day three postirradiation, donor mice were sacrificed, thymi were harvested, and thymocytes were isolated. iNKT cells were enriched by labeling samples with anti-CD8 magnetic beads and depleting CD8+ cells using an AutoMACS (Miltenyi Biotec). Cells were then stained for 10 minutes at 37°C in the dark with 10μM Cell Proliferation Dye eFuor450 (eBioscience) in PBS. Irradiated recipients were intravenously injected with 5–10 million cells. iNKT cell proliferation was analyzed in recipient spleen, liver, and blood samples on day 7 postinjection. In vitro iNKT Cell Simulation and Cytokine Analysis

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A 96-well plate was coated with 0.5 μg/well of α-mouse CD3ε and CD28 antibodies (eBioscience). Thymic iNKT cells were sorted using a FACSAria (BD Biosciences) and plated in triplicate at a concentration of 10,000 or 30,000 cells/well, depending on the experiment. The supernatant was collected 24 hours post-stimulation and cytokines were quantified using a Cytometric Bead Array Flex Sets (BD Biosciences) and analyzed with FCAP Array Software (BD Biosciences). For cytokine stimulation, 2 × 106 thymocytes were treated with PMA (200 ng/mL) and Ionomycin (5 μg/mL) for 2.5 hours at 37 °C in RPMI cell culture media with 10% FBS. Samples were stained with cell surface markers, fixed and permeabilized, and stained with anti-cytokine antibodies. Generation of Mixed Bone Marrow Chimera

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Jα18−/− recipient mice (CD45.2+) were lethally irradiated with 10.5 Gy and placed on oral Sulfamethoxazole and Trimethoprim (Hi-tech Pharmacal) treatment for two weeks. One day post-irradiation, donor bone marrow cells were harvested under sterile conditions from SHIP1+/− and SHIP1−/− animals (CD45.1+) and control C57BL/6 animals (CD45.2+). AntiCD5 and anti-DX5 magnetic beads were used to deplete mature T and NK cells using an AutoMACS (Miltenyi Biotec). Mixed bone marrow chimeric mice were generated by pooling cells from SHIP1+/− or SHIP1−/− animals with cells from C57BL/6 animals in a 1:1 ratio. 1×106 cells were injected intravenously into recipients and allowed to reconstitute for 8–10 weeks.


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Statistical Analysis

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All statistical analyses were accomplished with Prism Version 5.0 (GraphPad Software) or Excel (Microsoft) using unpaired two-tailed Student's t tests. A paired two-tailed Student's t test was used for samples from mixed bone marrow chimera. P value < 0.0001 ****, P value 0.0001 to 0.001 ***, P value 0.001 to 0.01 **, P value 0.01 to 0.05 *, and P value > 0.05 is not significant.

Results Germline deletion of SHIP1 hinders iNKT cell development

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SHIP1 (encoded by INPP5D) expression is an important regulator of PI3K activity. Loss of SHIP1 in mice leads to a number of hematopoietic defects, such as myeloid cell infiltration in the lungs and a severe inflammation of the small intestine that resembles human Crohn's disease (21, 27). Given its proposed role as a negative regulator of TCR signaling (28, 29), and because iNKT cell development is critically dependent on unique signals emanating from its semi-invariant TCR (30), we hypothesized that SHIP1 may play a role in iNKT cell development and functions. To minimize any pleiotropic effects, SHIP1−/− animals were sacrificed prior to seven weeks of age. Characterization of SHIP1-deficient mice revealed an increased frequency of iNKT cells in the thymus, yet decreased frequencies in the spleen and liver, compared to SHIP1+/+ and SHIP1+/− animals (Fig. 1A, Supplementary Fig. 1A). In contrast, the total number of iNKT cells was significantly decreased in the thymus and spleen (Fig. 1B). To exclude this being due to a relocation to other organs, we determined that the frequency of iNKT cells in the bone marrow, blood, inguinal lymph nodes, and mesenteric lymph nodes was also decreased (Supplementary Fig. 2A&B). We next wanted to determine whether SHIP1 deletion was causing iNKT cells to arrest at a specific stage of maturation. However, there was no difference in iNKT cell maturation between Stages 1–3, distinguished by NK1.1 and CD44 expression (Fig. 1C, Supplementary Fig. 1B). Notably, the frequency of the Stage 0 iNKT cells in SHIP1 deficient animals was modestly increased, while the total number was comparable to littermate controls (Supplementary Fig. 1C). These results indicated that residual iNKT cells are capable of undergoing normal development. We also examined if the absence of SHIP1 influenced the ratio of NKT1, NKT2 and NKT17 cell lineages. iNKT cells have recently been phenotypically classified according to their function and transcription factor expression, and are distinguished using anti-PLZF, anti-RORγt and anti-T-bet mAbs (31). When comparing thymic iNKT cells from SHIP1-deficient mice and littermate controls, we found a modest skew toward the NKT1 lineage (Fig. 2). A BALB/c positive control was used to define the gating strategy for these experiments, since they have robust populations of the three NKT lineages.

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Interestingly, when we examined the thymic T cell compartment, it revealed that T cell development was being affected globally. SHIP1−/− mice had increased frequencies of double negative (DN) and CD4 and CD8 single positive (SP) T cell populations, and a decreased frequency of CD4+CD8+ DP T cells, compared to SHIP1+/− and SHIP1+/+ controls (Fig. 3). However, although the mice had been crossed more than 15 times onto the B6 background, there was a spectrum in the severity of the impairment observed in the T cell compartment, most likely due to the pleiotropic effects of global SHIP1 loss (Fig. 3B).


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Similarly to T cells, iNKT cell frequency was also quite variable in the thymus of SHIP1deficient animals (Fig. 1A). Interestingly, SHIP1−/− mice with less severely affected T cell development were also those with less serious defects in their iNKT cell population (not shown). Overall, the data demonstrated that germline deletion of SHIP1 hindered both T cell and iNKT cell development. Extrinsic expression of SHIP1 rescues T cell development

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As mentioned above, germline deletion of SHIP1 results in massive myeloid infiltration of the lung, making it difficult to study the role of SHIP1 in lymphocytes without the influence of an inflammatory environment. In addition, the intrinsic role of SHIP1 in iNKT cell development cannot be examined in SHIP1-deficient animals. To circumvent these two issues, we generated a series of mixed bone marrow chimeras. SHIP1−/− bone marrow, which is on a congenic background, and wild-type C57BL/6 competitor bone marrow were transferred at a 1:1 ratio into lethally irradiated Jα18−/− recipients (Fig. 4A). Jα18−/− animals lack the Jα18 gene segment necessary for generation of the iNKT cell invariant α-chain. Therefore, all iNKT cells present in mixed bone marrow chimeric mice were derived from the two donor populations. Analysis of the chimeric mice indicated that conventional T cell development was globally intact. In contrast to the germline-deficient mice, the thymic T cell compartment of the chimera did not reveal significant differences in the DP, DN, or CD4 and CD8 SP populations (Fig. 4B). These data demonstrated that SHIP1 was required extrinsically during the development of conventional T cells. Similarly to conventional T cells, iNKT cells derived from C57BL/6 and SHIP1−/− donor populations had comparable frequencies in all the organs tested, suggesting that extrinsic expression of SHIP1 was sufficient for normal development of the iNKT cell compartment as well (Fig. 4C).


To confirm these results, we also analyzed mice that were conditionally deficient for SHIP1. In these mice (CD4CreSHIP1fl/fl, referred to as CD4Cre), a transgene for Cre recombinase driven by the cd4 enhancer/promoter/silencer (32, 33) allows specific gene deletion at the double negative 4 (DN4)/DP stages of T cell development. To verify that SHIP1 was efficiently deleted from T cells, splenic T cells and non T cell populations from CD4Cre and SHIP1fl/fl control animals (referred to as Control) were sorted for protein analysis by Western blot using an α-mouse SHIP1 primary antibody. Expression of SHIP1 in T cells was strongly reduced, indicating as expected, that INPP5D had been efficiently deleted from the T cell lineage in CD4Cre animals (Supplementary Fig. 2C). In agreement with the data from our chimeric mice, we found that conventional T cell development was globally intact, confirming that SHIP1 was not required intrinsically for T cell development (Supplementary Fig. 2D). The iNKT cell compartment was also intact in the thymus, spleen, and liver of CD4Cre and Control animals (Fig. 5A). In addition, analysis of the different developmental thymic iNKT cell stages did not show any abnormalities (Fig. 5B). Taken together, these data further illustrated that extrinsic expression of SHIP1 was sufficient to rescue iNKT and T cell development.


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Decreased number of iNKT cells in mice with germline deletion of SHIP1 is due to impaired proliferation

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SHIP1 has been shown to have opposing roles on myeloid and lymphoid cell proliferation (21, 34). Therefore, the reduced number of iNKT cells in the thymus and the spleen could potentially be the result of impaired proliferation in SHIP1-deficient animals. To evaluate the role of SHIP1 on iNKT cell proliferation, thymic iNKT cells from SHIP1−/− and wildtype C57BL/6 animals were labeled with Cell Proliferation Dye eFluor450 and injected intravenously into sublethally irradiated recipients. This lymphopenia-induced in vivo proliferation assay has shown that iNKT cells undergo extensive proliferation (35). We found that iNKT cells from SHIP1−/− animals had a decreased proliferative capacity on Day 7 compared to control wild-type cells in the liver, spleen, and blood (Fig. 6A). We also investigated steady state iNKT cell proliferation on Day 0 using intracellular Ki67 expression. In agreement with the impaired proliferation, we observed that SHIP1−/− thymic iNKT cells had significantly decreased Ki67 expression compared to C57BL/6 thymic iNKT cells (Supplementary Fig. 3A). Interestingly, SHIP1−/− animals with less affected T cell development and iNKT cell populations had comparable Ki67 expression to C57BL/6 controls (Supplementary Fig. 3B). We also observed thymic iNKT cells from CD4Cre and Control animals to have comparable Ki67 levels (Supplementary Fig. 3C). Notably, CD69 expression, a marker of early activation, was not affected in SHIP1-deficient iNKT cells (data not shown). Taken together, these data indicated that the lower number of iNKT cells in SHIP1−/− mice was due to a decreased proliferative potential, which could be rescued with extrinsic expression of SHIP1. iNKT cell cytokine production is decreased in the absence of SHIP1


We next sought to determine whether deletion of SHIP1 affected iNKT cell functions. iNKT cells are unusually poised to produce cytokines and can secrete a large amount ex vivo without priming. Thymic iNKT cells were sorted from SHIP1−/− and SHIP1+/− animals and stimulated with a combination of anti-CD3 and anti-CD28 mAbs for 24 hours and culture supernatants were then collected to measure cytokine production. Ligation of CD3 or CD28 on T cells has been shown to induce SHIP1 tyrosine phosphorylation (36). Using cytometric bead array flex sets, we measured the amount of cytokines produced by activated thymic iNKT cells. We found that IL-4, IFN-γ, IL-17A and TNF-α cytokine production from SHIP1−/− iNKT cells were significantly decreased compared to iNKT cells from littermate controls (Fig. 6B). To circumvent the developmental issues observed in mice with germline deletion of SHIP1, and to compare iNKT cells that develop in the same environment, we also stimulated iNKT cells that were sorted from mixed bone marrow chimeric mice. Similarly to iNKT cells from germline-deficient mice, we found that cytokine production was significantly decreased in SHIP1−/− samples, compared to C57BL/6 samples from the same mixed chimera (Fig. 6C). Thus, although SHIP1 was not required intrinsically for iNKT cell development, it was required for optimal iNKT cell cytokine production (37). We next wanted to determine whether SHIP1-deficient iNKT cells were capable of producing cytokines irrespective of TCR stimulation. To accomplish this, we stimulated thymocytes from SHIP1−/− animals and littermate controls for 2.5 hours with PMA and Ionomycin, which act downstream of SHIP1. Interestingly, there were also significant decreases in the


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frequency of iNKT cells producing IFN-γ and TNF-α (Supplementary Fig. 3D&E). Altogether, these data demonstrate that SHIP1-deficient iNKT cells are hyporesponsive.

Discussion

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The PI3K signaling pathway participates in a number of cellular processes, including activation, development, proliferation, and survival (11, 12). PI3K phosphorylates the D3 position of PI(4,5)P2 molecules to yield PI(3,4,5)P3 (38). PTEN and SHIP1 can prevent the initiation of these signals by converting PI(3,4,5)P3 to PI(4,5)P2 and PI(3,4)P2, respectively (39, 40). It has been shown that the absence of PTEN in T cells impairs the development of iNKT cells (41). Loss of regulation by SHIP1 has been shown to have differential effects on myeloid and lymphoid cells, including both NK and T cells (24, 26, 37, 39, 42, 43). However, the role of SHIP1 on iNKT cells is not well characterized. We found that SHIP1 is not required intrinsically for iNKT cell development, but is essential for optimal iNKT cell cytokine production. We also report that SHIP1 expression in trans is sufficient for the normal development of conventional T cells, at least in non-mucosal tissues (27). Regarding conventional T cells, there is some debate in the literature about the role of SHIP1. Some studies have reported that germline deletion of SHIP1 affects T cell development, while others found no major developmental issues (21, 24). In one study using a T cell-specific deletion model of SHIP1, no T cell developmental issues were observed (25). However, there was still expression of a near full-length version of SHIP1 that only lacked the enzyme domain (44), complicating the interpretation of these data. The findings presented here clarify these inconsistencies.


iNKT cells express a variety of inhibitory receptors, such as Ly49 molecules, CD94/ NKG2A, and PD-1 (45–47). In addition, mice conditionally deficient in PTEN express higher levels of Ly49C/I, which appears to be PIP3 dependent (41). Therefore, we hypothesized that SHIP1 deletion would affect iNKT cell development more severely than conventional T cells. Instead, we observed that germline deletion of SHIP1 had a variable effect on thymocyte development, ranging between a modest defect and a major alteration in the frequency of DN and DP cells (see Figure 2C). However, we found that the intensity of the iNKT cell developmental defect was linked to the strength of the T cell development defect (data not shown). Thus, the absence of SHIP1 did not appear to have a greater effect on iNKT cell development than conventional T cells. Notably, cell surface expression of Ly49 receptors on iNKT cells was not influenced by absence of SHIP1 (data not shown). Altogether, our data indicate that extrinsic expression of SHIP1 indirectly influences iNKT cell development. The reduction in the iNKT compartment is analogous to the impaired B and NK cell cellularity that is also observed in SHIP1−/− animals (42, 48). However, there are discordant findings regarding an autonomous role for SHIP1 in peripheral NK cell homeostasis using NCR1CreSHIPflox/flox mice (37) and mixed bone marrow chimeras (42). SHIP1 has been shown to participate in negatively regulating T cell signaling by affecting localization of Tec Kinase at the membrane, which is involved in cell proliferation following stimulation of the TCR (28). It has also been shown that T cells from SHIP1−/− animals have a poor proliferative response to TCR stimulation ex vivo (24). We found that SHIP1−/− thymic iNKT cells (or a subset of iNKT cells) have a decreased proliferative capacity in vivo


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and decreased Ki67 expression. However, animals with the least affected iNKT cell compartment had comparable Ki67 expression to C57BL/6 controls. Additionally, CD4Cre and Control animals had comparable Ki67 expression. Therefore, SHIP1 is required in trans for normal iNKT cell proliferation, similarly to conventional T cells (25). This explains the decreased number of iNKT cells found in SHIP1−/− animals. Although less likely, the decreased number of total DP cells could additionally result in poor positive selection of NKT cells.


Although iNKT cell development was unaffected in chimeric mice, we found that intrinsic absence of SHIP1 had an impact on iNKT cell cytokine secretion. The poor cytokine response of SHIP1-deficient iNKT cells was somewhat unexpected since SHIP1 is a negative regulator of intracellular signaling, but in line with work showing that it promotes, rather than inhibits IFN-γ production by NK cells (37, 49). However, there are several nonmutually exclusive possibilities that could explain this phenotype. First, it is conceivable that inhibitory receptor signaling via SHIP1 calibrates the threshold of iNKT cell reactivity. In fact, NK cells were also shown to be hyporesponsive in the absence of the phosphatases SHIP1 (42) and SHP-1 (50). Similarly to NK cells (26, 37, 51), the absence of SHIP1 may render iNKT cells hyporesponsive. Alternatively, the lower cytokine release from stimulated SHIP1-deficient iNKT cells could be due to a different differentiation program. For instance, it has been shown that a large proportion of SHIP1-deficient conventional T cells become phenotypically similar to regulatory T cells (Tregs) by expressing Foxp3 (24, 43). We first confirmed the previously observed increase of the Treg population in SHIP1deficient animals (Supplementary Fig. 4A&C). However, even though other conditions can cause regulatory iNKT cells (52, 53), we found that SHIP1-deficient iNKT cells do not acquire Foxp3 expression (Supplementary Fig. 4B&C). Another possibility is that the impaired iNKT cell cytokine production is the result of the expansion of a specific iNKT cell subset. However, this is unlikely since we found that SHIP1 deficient iNKT cells only skew slightly toward the NKT1 lineage. Taken together, our data indicate that deletion of the negative regulator SHIP1 leads to a decrease of iNKT cell cytokine secretion potential due to an alteration in the threshold for iNKT cell reactivity.

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The roles of other phosphatases, such as tyrosine phosphatases SHP-1 and SHP-2, may also contribute to iNKT cell activation. Additionally, isoforms of SHIP1 such as s-SHIP and SHIP-2, may participate in immune cell development and function. SHIP-2 is also expressed on hematopoietic cells and contains a 5-phosphatase activity (54), whereas embryonic stem cells and progenitor cells express s-SHIP (55). ENU-induced SHIP1 mutants (SHIP1el20) also affect s-SHIP and result in an enhanced impairment, compared to SHIP1−/− animals, including reduced T cell populations (56). The contribution of SHP-1, SHP-2, SHIP-2, and other phosphatases on iNKT cell development and functions still warrant further investigation.

Supplementary Material Refer to Web version on PubMed Central for supplementary material.


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Acknowledgments We thank Kevin Carlson for cell sorting.

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Author Manuscript Author Manuscript Figure 1. Loss of thymic and peripheral iNKT cells in SHIP1−/− mice

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(A) Frequencies of iNKT cell populations in the thymus, spleen, and liver from SHIP1+/+, SHIP1+/−, and SHIP1−/− mice. (B) Total iNKT cell numbers from each indicated organ. (C) Frequencies of thymic iNKT cells within the three stages of maturation, CD44loNK1.1− (Stage I), CD44hiNK1.1− (Stage II), and CD44hiNK1.1+ (Stage III). iNKT cells are defined as HSAloCD1tet+ in the thymus and TCRβ+CD1tet+ in the spleen and liver within the lymphoid gate. Black circles and bars: SHIP1+/+ (n=14), gray circles & bars: SHIP1+/− (n=18), and white circles & bars: SHIP1−/− mice (n=16). Data are pooled from at least 3 independent experiments and each dot is representative of 1 mouse. Error bars indicate SEM.

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Author Manuscript Author Manuscript Figure 2. SHIP1−/− mice have a more pronounced NKT1 phenotype than littermate controls

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(A) Representative staining of thymic NKT1, NKT2, and NKT17 populations from BALB/c control, SHIP1+/−, and SHIP1−/− mice using mAb for PLZF, ROR-γt, and T-Bet. (B) Frequency of thymic NKT1, NKT2, and NKT17 populations based on PLZF and ROR-γt expression. Black bars: BALB/c (n=3), grey bars: SHIP1+/− (n=9), and white bars: SHIP1−/− mice (n=9). iNKT cells are defined as TCRβ+CD1tet+ within the lymphoid gate. Data are pooled from 3 independent experiments and each dot is representative of 1 mouse. Error bars indicate SEM.


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Author Manuscript Author Manuscript Figure 3. SHIP1−/− mice have impaired conventional T cell development

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(A) and (B) Frequencies of thymic lymphocyte populations that are DN (CD4−CD8−), DP (CD4+CD8+), CD4 SP, and CD8 SP from SHIP1+/+, SHIP1+/−, and SHIP1−/− mice. (C) Representative staining of thymic T cell development, illustrating DN (CD4−CD8−), DP (CD4+CD8+), CD4 SP, and CD8 SP populations after the lymphoid gate in SHIP1+/+, SHIP1+/−, and SHIP1−/− mice. Black circles & bars: SHIP1+/+ (n=14), gray circles & bars: SHIP1+/− (n=18), and white circles & bars: SHIP1−/− mice (n=16). Data are pooled from at least 3 independent experiments and each dot is representative of 1 mouse. Error bars indicate SEM.


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Figure 4. Extrinsic expression of SHIP1 regulates iNKT cell and T cell populations

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(A) Diagram representing mixed bone marrow chimera generation. SHIP1−/− (CD45.1+) and C57BL/6 (CD45.2+) bone marrow cells were mixed at an equal ratio and intravenously injected into lethally irradiated Jα18−/− recipients. Recipients were analyzed 6 to 8 weeks following reconstitution. (B) Frequencies of thymic lymphocyte populations that are DN (CD4−CD8−), DP (CD4+CD8+), CD4 SP, and CD8 SP from mixed chimeric mice. (C) Frequencies of total iNKT cell populations in the thymus, spleen, and liver from C57BL/6 and SHIP1−/− donors in mixed chimeras. iNKT cells are defined as HSAloCD1tet+ in the thymus and TCRβ+CD1tet+ in the spleen and liver within the lymphoid gate. Black circles & bars: C57BL/6 competitor (n=9) and white circles & bars: SHIP1+/− (n=9). Data are pooled from at least 3 independent experiments and each dot is representative of 1 mouse. Error bars indicate SEM.


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Author Manuscript Author Manuscript Figure 5. Normal iNKT cell populations in mice conditionally deficient for SHIP1 in T cells

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(A) Representative staining of iNKT cells from the thymus, spleen, and liver of CD4Cre mice and littermate controls. (B) Frequencies of thymic iNKT cells within the three stages of maturation, CD44loNK1.1− (Stage I), CD44hiNK1.1− (Stage II), and CD44hiNK1.1+ (Stage III) from CD4Cre mice (n=8) and littermate controls (n=9). Data are pooled from 3 independent experiments and each dot is representative of 1 mouse. Black bars: Control mice and gray bars: CD4Cre mice. iNKT cells are defined as HSAloCD1tet+ in the thymus and TCRβ+CD1tet+ in the spleen and liver within the lymphoid gate. Error bars indicate SEM.


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Figure 6. SHIP1 deficiency extrinsically affects iNKT cell proliferation and intrinsically affects cytokine production

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(A) Day 7 labeling of pooled donor iNKT cells found in the liver, spleen, and blood of recipient animals from SHIP1−/− (CD45.1+) and C57BL/6 (CD45.2+) mice. iNKT cells are defined TCRβ+CD1tet+ in the liver, spleen, and blood in the lymphoid gate. Data are representative of 3 independent experiments. (B) Cytokine production was assessed in vitro using sorted thymic iNKT cells from SHIP1+/− and SHIP1−/− animals. Represents pooled data from four independent experiments, normalized to heterozygous control samples. (C) Cytokine production was assessed in vitro using sorted thymic iNKT cells from SHIP1−/− and competitor C57BL/6 populations from mixed chimera. Represents pooled data from two independent experiments, normalized to C57BL/6 control samples. Error bars indicate SEM.


Cutting edge: Discrete functions of mTOR signaling in invariant NKT cell development and NKT17 fate decision.

Essential functions for ID proteins at multiple checkpoints in invariant NKT cell development.

Tuberous sclerosis 1 promotes invariant NKT cell anergy and inhibits invariant NKT cell-mediated antitumor immunity.

Invariant NKT cell development: focus on NOD mice.

The regulatory role of invariant NKT cells in tumor immunity.

An essential role for medullary thymic epithelial cells during the intrathymic development of invariant NKT cells.

Invariant NKT cell response to dengue virus infection in human.

Vaccine development: NKT-cell adjuvants in conjugate.

TIM-4 is expressed on invariant NKT cells but dispensable for their development and function.

Leishmania infantum Exoproducts Inhibit Human Invariant NKT Cell Expansion and Activation.

NKAP Regulates Invariant NKT Cell Proliferation and Differentiation into ROR-γt-Expressing NKT17 Cells.

TNF superfamily receptor OX40 triggers invariant NKT cell pyroptosis and liver injury.

A new mouse strain for the analysis of invariant NKT cell function.

Invariant NKT cells: Killers and conspirators against cancer.

Nod1 and Nod2 enhance TLR-mediated invariant NKT cell activation during bacterial infection.

Targeted disruption of CD1d prevents NKT cell development in pigs.

Selective Conditions Are Required for the Induction of Invariant NKT Cell Hyporesponsiveness by Antigenic Stimulation.

Invariant NKT Cell Activation Induces Late Preterm Birth That Is Attenuated by Rosiglitazone.

Invariant NKT cells regulate the CD8 T cell response during Theiler's virus infection.

ITK tunes IL-4-induced development of innate memory CD8+ T cells in a γδ T and invariant NKT cell-independent manner.

Quantitative defects in invariant NKT cells and TLR responses in patients with hyper-IgE syndrome.

Structural and Functional Changes of the Invariant NKT Clonal Repertoire in Early Rheumatoid Arthritis.

Repeated Activation of Lung Invariant NKT Cells Results in Chronic Obstructive Pulmonary Disease-Like Symptoms.

Transfer of mRNA Encoding Invariant NKT Cell Receptors Imparts Glycolipid Specific Responses to T Cells and γδT Cells.