Journal of Visualized Experiments

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Materials List for:

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation 1,2

3

3

Emmanuel Bikorimana , Danica Lapid , Hyewon Choi , Richard Dahl

1,2,3

1

Harper Cancer Research Institute

2

Microbiology and Immunology, Indiana University School of Medicine

3

Department of Biological Sciences, University of Notre Dame

Correspondence to: Richard Dahl at [email protected] URL: http://www.jove.com/video/52022 DOI: doi:10.3791/52022

Materials Name

Company

Catalog Number

DMEM

Sigma/Aldrich

D5796

β-mercaptoethanol

Sigma/Aldrich

M3148

FBS (ES Screened)

Hyclone

SH30070.03E

FBS (Defined)

Hyclone

SH30070.03

Non-essential Amino Acids

Life Technologies

11140

L-Glutamine

Life Technologies

35050

Penn/Strep

Life Technologies

15070

Trypsin/EDTA

Life Technologies

25200

LIF ESGRO

Millipore

ESG1107

ACCUMAX

Millipore

SCR006

Trypsin/EDTA

Life Technologies

25200

Falcon Tissue Culture Plates 6-well Fisher Scientific

08-772-1B

Falcon Non-treated Plates 6-well

Fisher Scientific

08-772-49

Falcon Petri dish 10 cm

Fisher Scientific

08-757-100D

Falcon Petri dish 15 cm

Fisher Scientific

08-757-148

Falcon Tube 5 ml

Fisher Scientific

14-959-11A

Falcon Tube 5 ml with Cell Strainer Fisher Scientific Cap

08-771-23

White sterile resevoirs

U.S.A. Scientific

111-0700

Rat anti-mouse CD41 PEconjugated

Biolegend

133906

Rat anti-mouse CD117 APC/CY7conjugated

Biolegend

105826

RW4 ESCs

ATCC

CRL-12418

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