HHS Public Access Author manuscript Author Manuscript
J Allergy Clin Immunol. Author manuscript; available in PMC 2017 June 01. Published in final edited form as: J Allergy Clin Immunol. 2016 June ; 137(6): 1909–1913.e4. doi:10.1016/j.jaci.2016.01.036.
Respiratory syncytial virus and rhinovirus severe bronchiolitis are associated with distinct nasopharyngeal microbiota Jonathan M. Mansbach, MD, MPHa, Kohei Hasegawa, MD, MPHb, David M. Henke, MPHc, Nadim J. Ajami, PhDd, Joseph F. Petrosino, PhDd, Chad A. Shaw, PhDc, Pedro A. Piedra, MDe, Ashley F. Sullivan, MS MPHb, Janice A. Espinola, MPHb, and Carlos A. Camargo Jr, MD, DrPH, FAAAAIb,f
Author Manuscript
aDepartment
of Medicine, Boston Children’s Hospital, Boston, MA
bDepartment
of Emergency Medicine, Massachusetts General Hospital, Boston, MA
cDepartment
of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX
dThe
Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX eDepartment
of Molecular Virology and Microbiology and Pediatrics, Baylor College of Medicine,
Houston, TX fDivision
of Rheumatology, Allergy and Immunology, Department of Medicine, Massachusetts General Hospital, Boston, MA
Author Manuscript
Keywords bronchiolitis; respiratory syncytial virus; rhinovirus; Proteobacteria; Firmicutes; microbiota; cohort study; infants To the Editor: 1
Author Manuscript
Bronchiolitis is the leading cause of infant hospitalization in the US and cohort studies 1 suggest that up to 50% of these hospitalized infants will develop childhood asthma. The two most common viral etiologies of severe bronchiolitis (i.e. bronchiolitis requiring 1 hospitalization) are respiratory syncytial virus (RSV) and rhinovirus (RV). Although these two viruses have been the focus of most previous cohorts examining the association between bronchiolitis and asthma, RSV and RV infect infants through a respiratory tract colonized 2 with bacteria. , 3 Cross-sectional and prospective data suggest that the respiratory microbiota 2 may also play a role in the development of childhood asthma. To date, no studies have examined the relationship between the respiratory viruses, RSV and RV, and the respiratory microbiota of infants hospitalized with bronchiolitis. This knowledge gap has hindered
Correspondence: Jonathan M. Mansbach, MD, MPH, Boston Children’s Hospital, Main Clinical Building, #9157, 300 Longwood Avenue, Boston, Massachusetts 02115, phone 617-355-3191 | fax 617-730-0884, [email protected]. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Mansbach et al.
Page 2
Author Manuscript
development of new conceptual models regarding the bronchiolitis to asthma pathway in this very high-risk population.
Author Manuscript
The 35th Multicenter Airway Research Collaboration (MARC-35) is a 17-center, prospective cohort study of 1,016 infants (age
J Allergy Clin Immunol. Author manuscript; available in PMC 2017 June 01. Published in final edited form as: J Allergy Clin Immunol. 2016 June ; 137(6): 1909–1913.e4. doi:10.1016/j.jaci.2016.01.036.
Respiratory syncytial virus and rhinovirus severe bronchiolitis are associated with distinct nasopharyngeal microbiota Jonathan M. Mansbach, MD, MPHa, Kohei Hasegawa, MD, MPHb, David M. Henke, MPHc, Nadim J. Ajami, PhDd, Joseph F. Petrosino, PhDd, Chad A. Shaw, PhDc, Pedro A. Piedra, MDe, Ashley F. Sullivan, MS MPHb, Janice A. Espinola, MPHb, and Carlos A. Camargo Jr, MD, DrPH, FAAAAIb,f
Author Manuscript
aDepartment
of Medicine, Boston Children’s Hospital, Boston, MA
bDepartment
of Emergency Medicine, Massachusetts General Hospital, Boston, MA
cDepartment
of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX
dThe
Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX eDepartment
of Molecular Virology and Microbiology and Pediatrics, Baylor College of Medicine,
Houston, TX fDivision
of Rheumatology, Allergy and Immunology, Department of Medicine, Massachusetts General Hospital, Boston, MA
Author Manuscript
Keywords bronchiolitis; respiratory syncytial virus; rhinovirus; Proteobacteria; Firmicutes; microbiota; cohort study; infants To the Editor: 1
Author Manuscript
Bronchiolitis is the leading cause of infant hospitalization in the US and cohort studies 1 suggest that up to 50% of these hospitalized infants will develop childhood asthma. The two most common viral etiologies of severe bronchiolitis (i.e. bronchiolitis requiring 1 hospitalization) are respiratory syncytial virus (RSV) and rhinovirus (RV). Although these two viruses have been the focus of most previous cohorts examining the association between bronchiolitis and asthma, RSV and RV infect infants through a respiratory tract colonized 2 with bacteria. , 3 Cross-sectional and prospective data suggest that the respiratory microbiota 2 may also play a role in the development of childhood asthma. To date, no studies have examined the relationship between the respiratory viruses, RSV and RV, and the respiratory microbiota of infants hospitalized with bronchiolitis. This knowledge gap has hindered
Correspondence: Jonathan M. Mansbach, MD, MPH, Boston Children’s Hospital, Main Clinical Building, #9157, 300 Longwood Avenue, Boston, Massachusetts 02115, phone 617-355-3191 | fax 617-730-0884, [email protected]. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Mansbach et al.
Page 2
Author Manuscript
development of new conceptual models regarding the bronchiolitis to asthma pathway in this very high-risk population.
Author Manuscript
The 35th Multicenter Airway Research Collaboration (MARC-35) is a 17-center, prospective cohort study of 1,016 infants (age
