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Release of endogenous heat shock protein 72 on the survival of sepsis in rats Tsen-Ni Tsai, MS,a Tzu-Ying Lee, MS,a Maw-Shung Liu, DDS, PhD,a,b I-Chun Chuang, PhD,c Mei-Chin Lu, PhD,d Huei-Ping Dong, PhD,e Sheng-I. Lue, MS,b and Rei-Chen Yang, MD, PhDa,f,g,* a

Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan Department of Physiology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan c Department of Respiratory Therapy, Kaohsiung Medical University, Kaohsiung, Taiwan d Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung, Taiwan e Department of Physical Therapy, Fooyin University, Kaohsiung, Taiwan f Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan g Department of Pediatrics, Changhua Christian Hospital, Changhua, Taiwan b

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abstract

Article history:

Background: This study was undertaken to clarify the role of extracellular heat shock

Received 12 March 2015

protein 72 on the survival of sepsis and to determine possible factor(s) that may be

Received in revised form

responsible for it.

17 April 2015

Materials and methods: Sepsis was induced by cecal ligation and puncture. Changes in serum

Accepted 1 May 2015

levels of heat shock protein (Hsp72) and cytokines were determined during sepsis, and the

Available online 7 May 2015

results were correlated with the survival. Effects of heat pretreatment on Hsp72 expression in septic rat leukocytes and those of septic rat serum, lipopolysaccharide (LPS), and certain

Keywords:

cytokines on the release of Hsp72 in macrophage NR8383 cells were determined.

Extracellular heat shock protein 72

Results: Circulating Hsp72 levels were increased during the progress of sepsis (0, 5.5, 6.5, 10,

Monocyte

and 6.5 ng/mL at 0, 3, 6, 9, and 18 h after cecal ligation and puncture, respectively) and the

chemotactic/chemoattractant

increases were correlated positively with survival rates. LPS triggered the release of Hsp72

protein-1

in heat pretreated animals. Heat pretreatment increased Hsp72 expression in nonsepsis

Lipopolysaccharide

(þ535%, P < 0.01) and sepsis (þ116%, P

Release of endogenous heat shock protein 72 on the survival of sepsis in rats.

This study was undertaken to clarify the role of extracellular heat shock protein 72 on the survival of sepsis and to determine possible factor(s) tha...
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