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Release of endogenous heat shock protein 72 on the survival of sepsis in rats Tsen-Ni Tsai, MS,a Tzu-Ying Lee, MS,a Maw-Shung Liu, DDS, PhD,a,b I-Chun Chuang, PhD,c Mei-Chin Lu, PhD,d Huei-Ping Dong, PhD,e Sheng-I. Lue, MS,b and Rei-Chen Yang, MD, PhDa,f,g,* a
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan Department of Physiology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan c Department of Respiratory Therapy, Kaohsiung Medical University, Kaohsiung, Taiwan d Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung, Taiwan e Department of Physical Therapy, Fooyin University, Kaohsiung, Taiwan f Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan g Department of Pediatrics, Changhua Christian Hospital, Changhua, Taiwan b
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abstract
Article history:
Background: This study was undertaken to clarify the role of extracellular heat shock
Received 12 March 2015
protein 72 on the survival of sepsis and to determine possible factor(s) that may be
Received in revised form
responsible for it.
17 April 2015
Materials and methods: Sepsis was induced by cecal ligation and puncture. Changes in serum
Accepted 1 May 2015
levels of heat shock protein (Hsp72) and cytokines were determined during sepsis, and the
Available online 7 May 2015
results were correlated with the survival. Effects of heat pretreatment on Hsp72 expression in septic rat leukocytes and those of septic rat serum, lipopolysaccharide (LPS), and certain
Keywords:
cytokines on the release of Hsp72 in macrophage NR8383 cells were determined.
Extracellular heat shock protein 72
Results: Circulating Hsp72 levels were increased during the progress of sepsis (0, 5.5, 6.5, 10,
Monocyte
and 6.5 ng/mL at 0, 3, 6, 9, and 18 h after cecal ligation and puncture, respectively) and the
chemotactic/chemoattractant
increases were correlated positively with survival rates. LPS triggered the release of Hsp72
protein-1
in heat pretreated animals. Heat pretreatment increased Hsp72 expression in nonsepsis
Lipopolysaccharide
(þ535%, P < 0.01) and sepsis (þ116%, P