Articles in PresS. Am J Physiol Heart Circ Physiol (April 24, 2015). doi:10.1152/ajpheart.00168.2015
1
Regulation of myogenic tone and structure of parenchymal arterioles by
2
hypertension and the mineralocorticoid receptor.
3
Paulo W. Pires1,2, William F. Jackson1 and Anne M. Dorrance1
4 5
Author Contributions: PWP, WFJ, AMD conception and design of research;
6
PWP performed experiments; PWP analyzed data; PWP and AMD interpreted
7
results of experiments; PWP prepared figures; PWP drafted manuscript; PWP
8
and AMD edited and revised manuscript; PWP, WFJ and AMD approved final
9
version of manuscript.
10 11
Author Affiliations. 1Department of Pharmacology and Toxicology, Michigan
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State University, East Lansing, MI, 48824; 2Department of Pharmacology,
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University of Nevada School of Medicine, Reno, NV, 89557.
14 15
Running Head: Hypertension and cerebral parenchymal arterioles
16 17
Corresponding Author: Anne McLaren Dorrance, Ph.D.
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1355 Bogue Street, Room B340B Life Sciences Building
19
Department of Pharmacology and Toxicology
20
Michigan State University
21
East Lansing, MI, 48824
22
E-mail: [email protected]
23
Phone: +1 517 432 7403
24
Fax:
+1 517 353 8915
25
Copyright © 2015 by the American Physiological Society.
26
ABSTRACT
27
Proper perfusion is vital for maintenance of neuronal homeostasis and brain
28
function. Changes in the function and structure of cerebral parenchymal
29
arterioles (PAs) could impair blood flow regulation and increase the risk of
30
cerebrovascular diseases, including dementia and stroke. Hypertension alters
31
the structure and function of large cerebral arteries, but its effects on PAs remain
32
unknown. We hypothesized that hypertension increases myogenic tone and
33
induces inward remodeling in PAs; we further proposed that antihypertensive
34
therapy (AhT) or mineralocorticoid receptor (MR) blockade would reverse the
35
effects of hypertension. PAs from 18-week-old stroke-prone spontaneously
36
hypertensive rats (SHRSP) were isolated and cannulated in a pressure
37
myograph. At 50mmHg intraluminal pressure, PAs from SHRSP showed higher
38
myogenic tone (%Tone: 39.1±1.9 vs 28.7±2.5, p
1
Regulation of myogenic tone and structure of parenchymal arterioles by
2
hypertension and the mineralocorticoid receptor.
3
Paulo W. Pires1,2, William F. Jackson1 and Anne M. Dorrance1
4 5
Author Contributions: PWP, WFJ, AMD conception and design of research;
6
PWP performed experiments; PWP analyzed data; PWP and AMD interpreted
7
results of experiments; PWP prepared figures; PWP drafted manuscript; PWP
8
and AMD edited and revised manuscript; PWP, WFJ and AMD approved final
9
version of manuscript.
10 11
Author Affiliations. 1Department of Pharmacology and Toxicology, Michigan
12
State University, East Lansing, MI, 48824; 2Department of Pharmacology,
13
University of Nevada School of Medicine, Reno, NV, 89557.
14 15
Running Head: Hypertension and cerebral parenchymal arterioles
16 17
Corresponding Author: Anne McLaren Dorrance, Ph.D.
18
1355 Bogue Street, Room B340B Life Sciences Building
19
Department of Pharmacology and Toxicology
20
Michigan State University
21
East Lansing, MI, 48824
22
E-mail: [email protected]
23
Phone: +1 517 432 7403
24
Fax:
+1 517 353 8915
25
Copyright © 2015 by the American Physiological Society.
26
ABSTRACT
27
Proper perfusion is vital for maintenance of neuronal homeostasis and brain
28
function. Changes in the function and structure of cerebral parenchymal
29
arterioles (PAs) could impair blood flow regulation and increase the risk of
30
cerebrovascular diseases, including dementia and stroke. Hypertension alters
31
the structure and function of large cerebral arteries, but its effects on PAs remain
32
unknown. We hypothesized that hypertension increases myogenic tone and
33
induces inward remodeling in PAs; we further proposed that antihypertensive
34
therapy (AhT) or mineralocorticoid receptor (MR) blockade would reverse the
35
effects of hypertension. PAs from 18-week-old stroke-prone spontaneously
36
hypertensive rats (SHRSP) were isolated and cannulated in a pressure
37
myograph. At 50mmHg intraluminal pressure, PAs from SHRSP showed higher
38
myogenic tone (%Tone: 39.1±1.9 vs 28.7±2.5, p
