J. S~eroid Biochem. Molec. Biol. Vol. 40, No. 1-3, pp. 193-197, 1991
0960-0760/91 $3.00 + 0.00 Copyright © 1991 Pergamon Press plc
Printed in Great Britain. All rights reserved
R E G U L A T I O N OF G R O W T H OF LNCaP H U M A N PROSTATE T U M O R CELLS BY G R O W T H FACTORS AND STEROID H O R M O N E S A. L. G. SCHUURMANS,* J. BOLT, J. VELDSCHOLTE a n d E. MULDER t Department of Endocrinology and Reproduction, Erasmus University Rotterdam, Rotterdam, The Netherlands
Summary--The mitogenic activity of several growth factors on androgen responsive LNCaP human prostate tumor cells was studied. A two-fold stimulation of cell proliferation was observed after a culture period of 6 days in 1 ng EGF/ml, 10 ng TGF-~/ml or 20 ng basic FGF/ml. TGF-fl (0.02 ng/ml), which did not affect cell proliferation when added alone to the culture medium, inhibited the EGF- and TGF
0960-0760/91 $3.00 + 0.00 Copyright © 1991 Pergamon Press plc
Printed in Great Britain. All rights reserved
R E G U L A T I O N OF G R O W T H OF LNCaP H U M A N PROSTATE T U M O R CELLS BY G R O W T H FACTORS AND STEROID H O R M O N E S A. L. G. SCHUURMANS,* J. BOLT, J. VELDSCHOLTE a n d E. MULDER t Department of Endocrinology and Reproduction, Erasmus University Rotterdam, Rotterdam, The Netherlands
Summary--The mitogenic activity of several growth factors on androgen responsive LNCaP human prostate tumor cells was studied. A two-fold stimulation of cell proliferation was observed after a culture period of 6 days in 1 ng EGF/ml, 10 ng TGF-~/ml or 20 ng basic FGF/ml. TGF-fl (0.02 ng/ml), which did not affect cell proliferation when added alone to the culture medium, inhibited the EGF- and TGF
