THE JOURNAL OF INFECTIOUS DISEASES. VOL. 140, NO.2. AUGUST 1979 © 1979 by The University of Chicago. 0022-1899/79/4002-0029$00.75

DATA FORUM It is apparent from the reviews of many manuscripts that investigations often yield uniquely interesting and important data that by themselves do not comprise the basis for an expanded classical research paper with a high competitive priority for publication. Thus, critical reviewers note the usefulness of specific data but ask for additional experiments that would be valuable but are impossible to conduct in the context of available time

and expense. To provide .a forum in which such important specific data will not be lost but can be presented concisely, the Journal is initiating on a trial basis the "Data Forum." Here you will find a single table or figure with an expanded legend, no discussion, a summary statement, and as many as three references. We hope that it will stimulate research and provide results that will increase knowledge that can be confirmed and extended.

Regional B-Cell Responses to Inactivated Influenza Virus Vaccine in Ferrets From the Division of Virology, Bureau of Biologics, Food and Drug Administration, Bethesda, Maryland

Colin McLaren

Adult male ferrets were infected as described previously [I] with A/USSR/77 (HlNI) influenza virus or with a variety of recombinant strains possessing A/USSR/77 surface antigens. Four to six months later, the animals were bled and inoculated im in the upper left forelimb with 0.5 ml of whole-A/USSR/77 influenza virus vaccine (Merck, Sharp & Dohme, West Point, Pa.) containing --60 JLg of viral hemagglutinin. At intervals after inoculation, groups of two or four ferrets were anesthetized, and their blood collected into heparin. Peritracheal and left and right axillary lymph nodes and the spleen were separately removed. Lymphocytes were separated from the blood by centri Iugauon on lymphocyte separa-

tion medium (Litton Bionetics, Kensington, Md.), and cell suspensions were prepared from the lymph nodes and spleens [I]. These cells were tested for production of specific antibody in a direct hemolytic plaque assay [2] using sheep red blood cells (SRBCs) that had been treated with whole-A/USSR/77 influenza virus vaccine that had been heated at 60 C for 60 min to prevent elution of the virus from the SRBCs. The number of specific plaque-forming cellsj l O" viable cells in each preparation was determined (figure I). The dominant specific B-cell responses occurred in the left axillary lymph nodes that drain the inoculation site and in the spleens of the vaccinated animals. Lower numbers of antibody-forming cells were detected in peritracheal lymph nodes that drain the respiratory tract and in right axillary lymph nodes. In peripheral blood lymphocytes, responding B cells were detected only on day 4. These data suggest that antibody may

Please address requests for reprints to Dr. C. McLaren, Bureau of Biologics, Division of Virology, 8800 Rockville Pike, Bethesda, Maryland 20205.

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© 1979 by The University of Chicago. 0022-1899/79/4002-0017$00.75

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Regional B-cell responses to inactivated influenza virus vaccine in ferrets.

THE JOURNAL OF INFECTIOUS DISEASES. VOL. 140, NO.2. AUGUST 1979 © 1979 by The University of Chicago. 0022-1899/79/4002-0029$00.75 DATA FORUM It is ap...
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